RESUMEN
La ingesta diaria de hortalizas se relaciona con menor riesgo de cáncer, diabetes, enfermedades coronarias y obesidad, pero partes de estos alimentos como cáscaras, tallos y hojas no son reutilizadas, aumentando los desperdicios e impactando negativamente al medio ambiente. El objetivo de este trabajo fue establecer el valor nutricional de hojas y tallos de apio, betarraga y brócoli del Mercado Mayorista Lo Valledor en Santiago de Chile. Un total de 6 muestras se recolectaron desde puntos de ventas del Mercado Mayorista, 3 de hojas de hortalizas (apio, betarraga y brócoli) y 3 de tallos (apio, betarraga y brócoli). La recolección, preparación y entrega de las mismas se realizaron de acuerdo a protocolos indicados por el laboratorio encargado de realizar los análisis nutricionales. Las hojas de betarraga presentaron mayor contenido de proteínas (2,6 g), fibra dietética total (4,5 g) y fibra dietética insoluble (3,3 g) que los tallos, pero estos aportan más carbohidratos (7 g). Las hojas de apio tienen más calorías (20,4 kcal), proteínas (2,4 g), fibra dietética total (7,4 g), fibra dietética soluble (1,3 g) y fibra dietética insoluble (5,3 g) aunque los tallos presentan un contenido superior de carbohidratos (4,1 g). Finalmente, las hojas de brócoli destacan por su contenido de carbohidratos (9,0 g), proteínas (2,5 g), fibra dietética total (10,4 g) y fibra dietética insoluble (7,9 g). De acuerdo al valor nutricional obtenido, estos excedentes son una opción de alimento en la dieta diaria de las personas y su valorización debe ser contemplada.
The daily intake of vegetables is related to a lower risk of cancer, diabetes, coronary diseases and obesity, but parts of them such as peels, stems and leaves are not reused, increasing waste and negatively impacting the environment. The objective of this work was to establish the nutritional value of celery, beetroot and broccoli leaves and stems from the Lo Valledor wholesale market in Santiago, Chile. A total of 6 samples were collected from sales points of the Lo Valledor wholesale market, 3 from vegetable leaves (celery, beetroot and broccoli) and 3 from stems (celery, beetroot and broccoli). The collection, preparation and delivery of them were carried out according to protocols indicated by the laboratory in charge of carrying out the nutritional analyzes. The beet leaves had higher protein content (2.6 g), total dietary fiber (4.5 g) and insoluble dietary fiber (3.3 g) than the stems, but they provide more carbohydrates (7 g). Celery leaves had more calories (20.4 kcal), protein (2.4 g), total dietary fiber (7.4 g), soluble dietary fiber (1.3 g), and insoluble dietary fiber (5.3 g) although the stems have a higher carbohydrate content (4.1 g). Finally, broccoli leaves stand out for their content of carbohydrates (9.0 g), proteins (2.5 g), total dietary fiber (10.4 g) and insoluble dietary fiber (7.9 g). According to the nutritional value obtained, these surpluses are a food option in the daily diet of people and their valorization should be contemplated.
RESUMEN
Objective To develop a systematic chromatography separation method for flavonoids from Glycyrrhiza uralensis Fisch. (GU). Methods A new method for the separation of effective parts and monomers of flavonoids from GU by two-dimensional reversed-phase liquid chromatography was developed using the self-developed preparation chromatography plant system with independent intellectual property rights. Flavonoids compounds were enriched with specific adsorption materials. The separation conditions of the chromatography were optimized by the chromatographic separation expert system software, and the loading weight of samples and the enrichment times of a separation were investigated. Results The process of chromatography separation of flavonoids from GU had good precision and reproducibility with C18 as separation and enrichment solid phase and the methanol/water and acetonitrile/water as mobile phase of one-dimensional and two-dimensional chromatography system. The dilution solution which used for one-dimensional and two-dimensional enrichment chromatography was water. The flow rate of gradient elution and dilution enrichment solution was 21 mL/min. The sample loading amount of chromatography separation was 300 mg each time. A total of 16 flavonoids parts contained stable chemical composition were obtained by the repeatable separation method after three times of enrichment. Nine pure compounds were obtained and identified by NMR and MS, which were liquiritin, liquiritigenin, formononetin, echinatin, 7,4'-dihydroxyflavone, 4'-O-[β-D-apio-D-furanosyl-(1→2)-β-D-glucopyranosyl] liquiritigenin, isoliquiritigenin, glycyrol, and glycycoumarin. Conclusion The study can provide a certain reference value for the systematic separation and cognition for flavonoids from GU.