Methanolic extract of Abrus precatorius promotes breast cancer MDA-MB-231 cell death by inducing cell cycle arrest at G0/G1 and upregulating Bax

Objective: To determine the anti-proliferative activity of Abrus precatorius (A. precatorius) leaf extracts and their effect on cell death. Methods: A. precatorius leaves were extracted successively with hexane, ethyl acetate and methanol by Soxhlet extraction. Aqueous extract was prepared by decoction at 50 ℃. Extracts of A. precatorius leaves were used to treat selected cancer and normal cell lines for 72 h. Furthermore, 3-(4,5-dimethyl thiazol-2-yl) 2,5-diphenyl tetrazolium bromide assay was performed to determine cell viability. Analysis of cell cycle arrest, apoptosis assay and apoptosis protein expressions were determined by flow cytometry. Results: Methanolic extract of A. precatorius leaves showed the lowest IC50 on MDA-MB-231 cells at (26.40±5.40) μg/mL. Flow cytometry analysis revealed that cell arrest occurred at G0/G1 phase and the apoptosis assay showed the occurrence of early apoptosis at 48 h in MDA-MB-231 cells treated with methanolic extract of A. precatorius leaves. Methanolic extract of A. precatorius leaves induced apoptosis by upregulation of Bax, p53 and caspase-3 and downregulation of Bcl-2. Conclusions: Methanolic extract of A. precatorius leaves promotes MDA-MB-231 cell death by inducing cell cycle arrest and apoptosis possibly via the mitochondrial-related pathway.

The effect of acupunctural and moxibustional therapy on the apoptosis of breast cell MCF-7 / 中国现代普通外科进展

Objective:To observe the effect on the apoptosis of breast cancer cell MCF-7 with acupunctural and moxibustional therapy.Methods:Zusanli and Sanyinjiao neiguan in both sides of female Wistar mice were acupunctured and moxibusted.MCF-7 cell was cultirated with the serum.To detecte the cytoxicity with MTT,and detecte the apoptosic rate and Fas expression rate with flow cytometry after 72 hours.Results:The Fas expression was obviously increased upto 57.70% after 72h.The proliferation of MCF-7 was inhibited remarkably.The inhibition rate were 17.55%,24.71% and 31.85% after 12h,24h and 72h.The apoptosic rate were 8.72% and 10.18% after 72h,remarkably higher than the control group(P

The effect of TFu on the apoptosis and proliferation of FRH-0201 cholangiocarcinoma cell line / 中国现代普通外科进展

Objective:To investigate the effect of TFu on FRH-0201 cell and to test new drugs for the chemotherapy of cholangiocarcinoma.Methods:Morphological changes were observed by light microscopy and fluorescence microscopy.The effect of TFu on FRH-0201 cell was examined by clone formation.Cell cycle and apoptosis were examined by flow-cytometric analysis.DNA ladder was used to detect apoptosis.To compare the drug susceptibility of TFu with 5-Fu and several other common drugs by MTT colorimetric assay.Results:TFu significantly inhibited the proliferation and clone formation of FRH-0201 cell line within concentration of 0.016~4mmol/L.The inhibition ratio was dependent on ascending concentration and time,and that is higher than 5-Fu with the same concentration.The morphological character of apoptosis was observed.Flow cytometric analysis indicated that TFu induced a G_1 phase arrest and apoptosis more than 5-Fu.ADM,DDP and TFu were the sensitive drugs to FRH-0201 cel line,and the combination of them can reach the highest inhibition ratio.Conclusion:These results suggests that TFu can more significantly inhibited the proliferation of FRH-0201 cell line than 5-Fu.The mechanism may be related to the cell cycle arrest in association with apoptosis,which may provide a new approach for treating cholangiocarcinoma.