RESUMEN
Objective: An HPLC method was established to determine the content of calycosin-7-O-β-D-glucoside and formononetin in Astraglus membranaceus var. mongholicus. To study the influence of habitats, processing methods, and planting ages on the quality of A. membranaceus var. mongholicus. Methods: The HPLC condition was as follows: column: Dikma C18 (250 mm×4.6 mm, 5 μm); mobile phase: Acetonitrile-0.2% formic acid (gradient elution); flow speed: 1.0 mL/min; detection wavelength: 260 nm; temperature of column: 30℃. Results: Comparing the content of calycosin-7-O-β-D-glucoside and formononetin in 40 samples of A. membranaceus var. mongholicus from 11 different habitats, we could get the following: The processing methods of A. membranaceus var. mongholicus were cleaning and natural drying; Bayinnaoer city Wu Qianqi ming'an town head part was the best habitat for A. membranaceus var. mongholicus. The quality of A. membranaceus var. mongholicus cultivars grown for two years and three years had no difference. The quality of wild species was superior cultivars. Conclusion: The method is simple and rapid for determination, the contents of calycosin-7-O-β-D-glucoside and formononetin in A. membranaceus var. mongholicus. It can be used as a scientific and reliable basis for formulating the reasonable standard of cultivating and processing methods of A. membranaceus var. mongholicus.
RESUMEN
Objective: To compare the effects of Na2CO3 and NaCl + Na2CO3 at different concentration on the seed germination and seedling physiological characteristics of Astraglus membranaceus var. mongholicus, analyze the stress degrees of the both two on A. membranaceus var. mongholicus, and search for the effective ways for improving the resistance of seeds and seedlings under salt stress. Methods: Several physiological indexes of A. membranaceus var. mongholicus seeds under different salt treatments, such as the germination vigor (Gv), germination rate (Gr), relative germination rate, and relative salt damage rate were measured. And other indexes of the seedlings like chlorophyll contents, soluble protein contents, the contents of malondialdehyde (MDA), the activities of superoxide dismutase (SOD), and peroxidase (POD) were also measured. Results: Na2CO3 and NaCl + Na2CO3 at lower concentration could promote the seed germination but inhibit the seed germination at higher concentration. The inhibition was increased with the concentration increasing. The germination indexes of each treatment had the significant differences compared with the control (P < 0.05). With the salt concentration increasing, the contents of chlorophyll and the soluble protein decreased, which was negatively correlated with the concentration. The content of MDA was increased and positively correlated with the concentration. The change trend of SOD activity showed the first increase and then decreased with the salt concentration increasing, which was similar to that of POD. The activities of both SOD and POD reached the peak at 50 mmol/L. Conclusion: The stress of NaCl + Na2CO3 is higher than that of Na2CO3 on the seeds and seedlings of A. membranaceus var. mongholicus.