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@#AIM: To explore the characteristics and application value of ultra-wide-field auto-fluorescence in Stargardt disease. <p>METHODS: A retrospective study of clinical characteristics and genetically confirmed Stargardt disease patients, using Optos P200Tx for ultra-wide-field auto-fluorescence imaging, and comparing the imaging features of traditional fundus color photographs, fundus 55° auto-fluorescence, and optical coherence tomography, to evaluate the ultra-wide-field auto-fluorescence in Stargardt disease image characteristics and advantages of clinical application. <p>RESULTS:All 76 eyes(100%)had high posterior auto-fluorescence, while only 42 eyes(55%)of traditional 55° auto-fluorescence showed obvious posterior high auto-fluorescence. Sixty-six eyes(87%)of the 76 eyes showed different numbers of spots, which were distributed from the posterior pole to the peripheral retina. UWAF can show clearer and more number of retinal spots than fundus color photographs, and more completely shows the number and distribution of spots area. All 76 eyes(100%)of the patients showed the oval low auto-fluorescence area induced by retinal pigment epithelium(RPE)atrophy in the center of the macula. As the disease progressed, the atrophy area expanded and the low fluorescence area expanded accordingly. Ultra-wide-field auto-fluorescence can completely display the atrophy range and area, but it cannot display in dystrophy depth. In 48 eyes(63%)ultra-wide-field auto-fluorescence, strong background auto-fluorescence was seen extending from the macula to the nasal and inferior temporal of the optic disc, forming a clear approximately vertical dividing line below the optic disc.<p>CONCLUSION: Ultra-wide-field auto-fluorescence changes in Stargardt are not limited to the posterior pole and may extend more peripherally. Ultra-wide-field imaging is a useful tool for the assessment of patients with Stargardt macular dystrophy.
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Objective: The condition of the resected margin in oral squamous cell carcinoma continues to be an important prognostic factor; the use of optic technology could help surgeons in determining the margin status at real time. This study aims to evaluate Oral ID, a hand held device that uses the principal of auto-fluorescence to determine surgical safe margins in patients with oral squamous cell carcinoma, and to compare the results with those of the conventional 1 cm margin method. Material and Methods: This study was a descriptive, comparative analytical study carried out at Khartoum Dental Teaching Hospital and Oral Histopathology Diagnostic Laboratory, Faculty of Dentistry, University of Khartoum. A total of 92 margins obtained from 31 patients, 46 margins were taken by Oral ID and the other 46 were taken by the traditional 1cm method. All margins were examined histologically with conventional Hematoxylin and Eosin stain. Results: It was found that all tumors showed fluorescence loss; A significant association was found between the use of Oral ID and obtaining a free margin P (0.02) the sensitivity of Oral ID was found to be 74% the specificity was found to be 89%. Ten out of the 46 margins obtained by fluorescence showed mild dysplasia and two margins showed high grade dysplasia. The 46 margins obtained by the traditional 1cm margin showed different field alterations two were involved, one was close, five showed high grade dysplasia and 14 showed mild dysplasia yielding a specificity of 52.2%. Conclusion: Using Oral ID for surgical margin assessment increases the accuracy to 74% compared to the conventional method which was found to be 52.2%. The results of the device are comparable to other auto-fluorescence devices of different trademarks. Further development of the device to help overcome its limitations is strongly advised (AU)
Objetivo: A condição da margem ressecada no carcinoma oral de células escamosas continua sendo um importante fator prognóstico; o uso de tecnologia óptica pode ajudar cirurgiões a determinar o status da margem em tempo real. O objetivo deste estudo é avaliar o Oral ID, um aparelho portátil que utiliza o princípio da autofluorescência para determinar margens de segurança cirúrgicas em pacientes com carcinoma oral de células escamosas, e comparar os resultados com o método convencional de margem de 1 cm. Material e Métodos: Este estudo foi um estudo descritivo, analítico e comparativo realizado no Khartoum Dental Teaching Hospital e no Laboratório de Diagnóstico de Histopatologia Oral da Faculdade de Odontologia, Universidade de Khartoum. Um total de 92 margens foram obtidas de 31 pacientes, 46 margens foram obtidas por Oral ID e as outras 46 foram obtidas pelo método tradicional de 1 cm. Todas as margens foram examinadas histologicamente com coloração convencional de Hematoxilina e Eosina. Resultados: Verificou-se que todos os tumores apresentaram perda de fluorescência; uma associação significativa foi encontrada entre o uso de Oral ID e a obtenção de uma margem livre P (0,02), a sensibilidade de Oral ID foi de 74% e a especificidade de 89%. Dez das 46 margens obtidas por fluorescência mostraram displasia leve e duas margens mostraram displasia de alto grau. As 46 margens obtidas pela margem tradicional de 1cm apresentaram diferentes alterações de campo, duas estavam envolvidas, uma estava próxima, cinco apresentaram displasia de alto grau e 14 apresentaram displasia leve com especificidade de 52,2%. Conclusão: O uso de Oral ID para avaliação da margem cirúrgica aumenta a acurácia para 74% em comparação com o método convencional, que foi encontrado em 52,2%. Os resultados do dispositivo são comparáveis a outros dispositivos de autofluorescência de diferentes marcas comerciais. O desenvolvimento do dispositivo para ajudar a superar suas limitações é fortemente recomendado. (AU)
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Humanos , Diagnóstico , Imagen Óptica , Carcinoma de Células Escamosas de Cabeza y Cuello , NeoplasiasRESUMEN
Purpose: The study was conducted to compare different methods of detection of pathogenic protozoan parasites in stool specimens of People Living with HIV/AIDS (PLHA). Materials and Methods: Stool specimens of 242 HIV sero-positive patients were examined using the wet mount technique, modified Ziehl-Neelsen's (ZN) staining, auto-fluorescence and auramine fluorescence staining. Patient specimens, 94 and 40 out of 242, were also subjected to Giardia antigen detection using an enzyme immunoassay and Cryptosporidium antigen detection by immuno-chromatography, respectively. For calculation of sensitivity, specificity, positive and negative predictive values, light microscopy of wet mounts and modified ZN stained smears for Giardia and Coccidia, respectively, were considered as gold standards. Results: Sensitivity of auto-fluorescence, auramine-O staining and antigen detection techniques was found to be 100% as compared to the routine standards. The specificity of auto-fluorescence was 90.6% and 100% for Cyclospora and Isospora, respectively; that of auramine-O staining was 98.9% for Cryptosporidium, 99.30% for Cyclospora and 100% for Isospora; and that of antigen detection was 90.6% and 97.7% for Cryptosporidium and Giardia, respectively. Conclusion: In laboratories requiring screening of large number of stool specimens for detection of protozoan parasites, fluorescence microscopy and antigen detection can be useful techniques. Confirmation of positive results, however, needs to be done with the standard techniques.
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To obtain the criterion of Laser induced Autofluorescence (LIF) spectroscopy in the differentiation of normal lung tissue and lung cancer and study the feasibility of LIF spectroscopy in the diagnosis of lung cancer, the LIF spectra of normal lung and lung cancer in 42 surgical specimens have been measured with a detecting system which consists of an YAG laser(wavelength 355nm) and an optical multichannel analyzer(OMA). Spectroscopic differences between normal lung and cancerous tissues have been found which could be used as a criterion to distinguish from them . The pathological examinations were done to compare with the criterion. The results showed:① The location of the principal spectral peaks of the normal lung tissue (470.8?6.3)nm and lung cancer ( 463.7 ?4.8)nm are different( P