Identificación de microorganismos anaerobios reactivos a la prueba de Bana antes y después de la terapia periodontal no quirúrgica en pacientes diabéticos / Identification of anaerobic microorganisms reactive to the Bana test before and after non-surgical periodontal therapy in diabetic patients

RESUMEN La prueba BANA determina la actividad proteolítica de las principales bacterias periodontales del complejo rojo, la facilidad y la versatilidad clínica de la prueba la ha convertido en una opción viable para valorar el tratamiento periodontal. El Objetivo de esta investigación es la identificación de microorganis-mos, anaerobios reactivos a la prueba BANA antes y después de la terapia periodontal no quirúrgica en pacientes diabéticos. Materiales y Métodos: para este estudio se trabajó con pacientes que acuden al Club de Diabéticos del Centro de Salud N.- 4 de Chimbacalle perteneciente al Ministerio de Salud Pública del Distrito Metropolitano de Quito. Se realizó un muestreo no probabilístico a conveniencia de un total de 30 pacientes diabéticos con edades de 35 a 75 años de edad de ambos sexos, a los cuales se les realizó el análisis microbiológico BANA, antes y después de la terapia periodontal no quirúrgica. Se realizó un estudio prospectivo antes de la terapia periodontal no quirúrgica, y un mes después en pacientes diabéticos con periodontitis crónica en sitios con profundidades de sondaje y nivel de inserción ≥ 5mm. Resultados: los resultados del estudio no mostraron diferencias significativas entre los pacientes del estudio antes y después de la terapia periodontal no quirúrgica para la prueba microbiológica BANA. Conclusiones: La terapia periodontal no quirúrgica es un tratamiento eficaz en la eliminación de la enfermedad periodontal siempre y cuando exista la colaboración del paciente. Los resultados de la prueba microbiológica en este estudio no demostraron diferencias significativas

ABSTRACT The BANA test determines the proteolytic activity of the main periodontal red complex bacteria, the ease and clinical versatility of the test has made it a viable option for assessing periodontal treatment. The objective of this research is the identification of anaerobic microorganisms reactive to the BANA test before and after non-surgical periodontal therapy in diabetic patients. Materials and Methods: For this study we worked with patients attending the Diabetic Club of the Health Center No. 4 of Chimbacalle belonging to the Ministry of Public Health of the Metropolitan District of Quito. A non-probabilistic convenience sampling of a total of 30 diabetic patients between 35 and 75 years of age of both sexes was carried out, who underwent BANA microbiological analysis before and after non-sur-gical periodontal therapy. A prospective study was performed before non-surgical periodontal therapy, and one month after in diabetic patients with chronic periodontitis in sites with probing depths and insertion level ≥ 5mm. Results: the results of the study showed no significant differences between study patients be-fore and after non-surgical periodontal therapy for the microbiological BANA test.Conclusions: Non-surgical periodontal therapy is an effective treatment in the elimination of periodontal disease as long as there is patient cooperation. The results of the microbiological test in this study showed no significant differences.

Ethnobotanical profiling and floristic diversity of Bana Valley, Kotli (Azad Jammu and Kashmir), Pakistan

OBJECTIVE: To document the medicinal and other folk uses of native plants of the Bana Valley of district of Kotli-Azad Jammu Kashmir with a view to preserve the ethnobotanical knowledge of this area and to develop an ethnobotanical inventory of the species diversity. METHODS: The fieldwork was conducted during a period of one year. Data were collected through semi-structured questionnaire and interview of the informants including indigenous people, tribal people, and traditional health practitioners residing in the study area. RESULTS: The present study documented etnobotanical uses of 86 plant species belonging to 81 genera and 47 families. This study revealed that most of the species were used medicinally (74 spp; 42.29%). Leaves found to be the most frequently used part (56 spp., 36.13%) for preparation of indigenous recipes and fodder purpose. CONCLUSIONS: The current research provides a huge lump of ethnobotanical knowledge and depicts strong human-plant interaction. It is an urgent need to document indigenous uses of plants for future domestication.

Flavoring agents present in a dentifrice can modify volatile sulphur compounds (VSCs) formation in morning bad breath

This study aimed to evaluate the effects of a flavor-containing dentifrice on the formation of volatile sulphur compounds (VSCs) in morning bad breath. A two-step, blinded, crossover, randomized study was carried out in 50 dental students with a healthy periodontium divided into two experimental groups: flavor-containing dentifrice (test) and non-flavor-containing dentifrice (control). The volunteers received the designated dentifrice and a new toothbrush for a 3 X/day brushing regimen for 2 periods of 30 days. A seven-day washout interval was used between the periods. The assessed parameters were: plaque index (PI), gingival index (GI), organoleptic breath scores (ORG), VSC levels (as measured by a portable sulphide monitor) before (H1) and after (H2) cleaning of the tongue, tongue coating (TC) wet weight and BANA test from TC samples. The intra-group analysis showed a decrease in ORG, from 3 to 2, after 30 days for the test group (p < 0.05). The inter-group analysis showed lower values in ORG, H1 and H2 for the test group (p < 0.05). There was no difference between the amount of TC between groups and the presence of flavor also did not interfere in the BANA results between groups (p > 0.05). These findings suggest that a flavor-containing dentifrice seems to prevent VSCs formation in morning bad breath regardless of the amount of TC in periodontally healthy subjects.

Avaliação da transmissibilidade de microrganismos periodontopatogênicos entre mães e filhos utilizando-se o teste BANA / Periodontophatogens microorganisms transmission between mother and children using the BANA test evaluation

Descobertas recentes sugerem que microrganismos periodontopatogênicos são transmitidos entre membros de uma mesma família, pois há verificações da semelhança de linhagens de bactérias obtidas de seus integrantes, principalmente da mãe. Assim, o objetivo foi verificar se havia alguma correlação significativa entre os resultados do Teste BANA (Perioscan), obtidos em amostras de placa dentária subgengival de 54 crianças, entre 6 e 9 anos, e suas mães, todos residentes no município de Barretos−S.P. As amostras de placa subgengival foram colhidas com curetas periodontais de 432 sítios. Após a análise dos dados, os resultados do Teste BANA demonstraram que 67 sítios de crianças (31%) apresentaram reatividade positiva, enquanto que nas mães o número foi de 85 (39,3%). Em relação a negatividade do BANA nas crianças e suas mães, foi, respectivamente, de 149 (69%) e de 131 (60,7%) sítios, o que demonstrou a existência de uma relação estatisticamente significativa entre as variáveis BANA de mãe e filho. Os resultados do presente estudo permitiram sugerir que a presença de bactérias periodontopatogênicas nos filhos está relacionada à presença das mesmas bactérias nas mães, e que possível relacionamento de alterações periodontais nas mães podem representar significado importante no estabelecimento das patologias periodontais nos filhos

Recent researchs suggest that periodontophatogens microrganisms are transmitted among the members of the same family, because it could be observed the likeness of the lineages of obtained from bacterias of family members, mainly of the mother. The purpose of this study was to verify if there was some significant correlation between the results of the Test BANA (Perioscan), obtained in samples of subgingival dental plaque of 54 children between 6 and 9 years and their mothers, resident in the municipal district BarretosS.P. The samples of the subgingival plaques were picked up with periodontal instruments in 432 sites. After the statistical analysis of the data, the BANA Test results showed that in the children 67 (31%) of the sites presented positive reactivity while in the mothers 85 (39,3%) and in relation to the negativity of the BANA test, it was 149 (69%) e 131 (60,7%) respectivilly, this showed a statistically significant relationship between mother's and son BANA variable. The results of the present study let us to suggest that the presence of bacterial periodontophatogens in the children is related with the presence of the same bacterias in their mothers, and that a possible relationship of the periodontal alterations in the mothers can be an important factor in the establishement of periodontal pathologies in children