Expression of microRNA-544 in hepatocelluar carcinoma (HCC) and its effect on malignant behaviors of HCC cells / 第二军医大学学报

To study the expression of microRNA-544 (miR-544) in hepatocelluar carcinoma (HCC) tissues and its effect on proliferation, apoptosis, colony formation and sphere-forming potential of HCC cells, so as to explore the role of miR-544 in carcinogenesis and development of HCC. Methods Quantitative real-time PCR was used to detect the expressions of miR-544 in liver tissues from rats treated with diethylnitrosamine (DEN), in HCC and adjacent non-tumor tissues from patients, and in HCC spheres after sphere-forming culture. After HCC cell line Hep3B being transfected with miR-544 mimic or miR-544 inhibitor, cell counting kit-8 (CCK-8) was used to detect the proliferation of the HCC cells, colony-formation assay was used to observe the colony-formation ability of the HCC cells, and flow cytometry was used to analyze the cell apoptosis of HCC cells with propidium iodide staining. The sphere-formation assay was performed to determine the sphere-forming potential of HCC cells transfected with miR-544 mimic. Results MiR-544 expression was significantly down-regulated in the HCC tissues of DEN-treated rats (versus the control rats without modelling, P<0.05) and HCC tissues of patients (versus adjacent non-tumor tissues, P<0.01). Overexpression of miR-544 significantly inhibited proliferation (P<0.05, P<0.01) and colony-formation potential (P<0.05) of HCC cells, and also significantly promoted the apoptosis of HCC cells at 72 h (P<0.01). While inhibition of miR-544 in HCC cells significantly promoted the cell proliferation (P<0.01) and colony-formation potential (P<0.05). MiR-544 expression was significantly down-regulated in sphere-forming HCC cells during enrichment culture of liver cancer stem cells (P<0.05), and overexpression of miR-544 inhibited the sphere-formation of HCC cells (P<0.05). Conclusion MiR-544 expression is reduced in HCC tissues and can inhibit the malignant biological behaviors of HCC cells, indicating its inhibitory roles in the development and progression of HCC.

The biological behavior and significance of aging bone mesenchymal stem cells / 国际生物医学工程杂志

Bone marrow mesenchymal stem cells (BMSCs),which can be isolated from organs and tissues,are cell populations with high degree of plasticity,similar to hematopoietic stem cells in bone marrow,and can be in vitro cultured,induced and amplified.BMSCs are increasingly used in gene therapy,cell therapy and tissue engineering.BMSCs have been currently studied in a number of autologous transplantations,while not all BMSCs are suitable for autologous transplantation.BMSCs exhibit biological aging gradually when cultured in vitro.Biological aging can be divided into age-induced senescence and long-term passage induced senescence.Aging BMSCs demonstrate changes in biological behaviour which reduces the success rate of autologous transplantation.In this review,biological aging BMSCs are discussed in order to provide help for the transplantation of BMSCs.

Biological behavior and disease pattern of carcinoma gallbladder shown on 64-slice CT scanner: A hospital-based retrospective observational study and our experience.

Purpose: The aim of this diagnostic observational study was to assess the spread and biological behavior of gallbladder cancer using 64-slice computerized tomography (CT) scanner in this particular geographic belt (eastern Uttar Pradesh, western Bihar, and northern Madhya Pradesh provinces of North India). Indians are ethnically and culturally different from their Western counterparts among whom the incidence of this disease is comparatively low. Subjects and Methods: After systemic examination, all patients (87) were subjected to ultrasonographic examination. All cases were histopathologically proven. Confirmed cases were subjected to volumetric CT examination of abdomen and pelvis, plain, post contrast and delayed phase. Results: Majority of the cases were adenocarcinoma. There was female preponderance with majority belonging to fifth and sixth decades. Commonest presentation was diffuse, irregular, enhancing wall thickening in 49.4%. Majority had invasion of liver parenchyma (74.7%). Cholelithiasis was seen in 48.3% cases. Invasion of biliary radicals was high (13.8-18.4%). Eleven cases had invasion of portal vein and tumor thrombus, with hepatic artery invasion in one case. In two cases, both hepatic artery and portal vein invasion was seen. Portal and peripancreatic nodal metastasis was seen in 58.5%. Distant metastasis was reported. Conclusion: Few studies involving the Indian population have attempted to use multi-row detector CT to define the biological behavior of carcinoma gallbladder. The opinion whether the pathology is operable or non-operable can reasonably be given. This large-scale, single-center study gives insight about the epidemiology and biological behavior of carcinoma gallbladder.

Expressions of Ki-67 and p53 in pulmonary sclerosing hemangioma and the clinical significance / 肿瘤研究与临床

ObjectiveTo study the expressions of Ki-67 and p53 in the surface cells and polygonal cells in pulmonary sclerosing hemangioma(PSH)and investigate the relation of cell proliferation index and biological behaviour of the tumor.MethodsDouble-staining immunohistochemistry was used to detect the expressions of Ki-67 and CK8/18 protein. Double immunofluorescence staining was used to detect the expressions of p53 and AE1/AE3 protein. ResultsThe positive signal of AE1/AE3 and CK8/18 were localized in cytomembrane of surface cells. The positive signal of Ki-67 and p53 were localized in cell nucleus of the two kinds of cells.The positive rate of Ki-67 was under 1％ in surface cells and 1％-10 ％ in polygonal cells. p53 protein was mainly expressed in polygonal cells (6/9, 33.3 ％) and only exsited in seldom surface cells.ConclusionThere are differences on cellular morphous and immunophenotype between the surface cells and polygonal cells.The proliferation index and gene mutation are all predominant in polygonal cells than in surface cells. The biological behaviour of PSH maybe mainly be decided by the polygonal cells.

Comportamento biológico de matriz scaffold acrescida de células progenitoras na reparação óssea / Bone marrow progenitor cells enriched scaffold biological behavior in bone repair

A engenharia tecidual desenvolve e manipula células ou tecidos para a reconstrução de novos órgãos e tecidos. A perda ou a falência da função de partes teciduais alteradas ou lesadas torna-se um problema severo à saúde animal e uma indicação para tratamento é o transplante para substituir a função biológica perdida. Vários animais são afetados por estes problemas, razões estas que indicam a necessidade de desenvolvimento de terapias alternativas para tratar perdas ósseas. Estudos experimentais contribuem para avanços da medicina regenerativa, como a engenharia de tecidos, com o objetivo de desenvolver suportes sintéticos ou utilizar arcabouços naturais, conhecidos como scaffold, que substituam as funções fisiológicas do tecido ósseo. Atualmente para, a obtenção destes objetivos utilizam-se também, de forma genérica, combinações específicas com células progenitoras (CP) da medula óssea (MO) do próprio paciente ou doador que são distribuídas em um material de suporte tridimensional com propriedades adequadas, gerando um material híbrido cujas características podem ainda ser moduladas para então ser reinserido no paciente. O objetivo desta revisão é apresentar informações sobre o comportamento biológico de materiais sintéticos e naturais apropriados para implantes ósseos, acrescidos ou não de CP, para o estímulo do processo reparativo de tecido ósseo.

Tissue engineering involves the manipulation and the development of cells aiming to create and/or regenerate organs and tissues. Function failure of injuried tissues is a severe animal health problem, nevertheless, the transplantation has been indicated to treat and replace the biological function. A large amount of animals are affected by those problems, and for this reason it is necessary to find alternative therapies to treat bone losses. Studies such those involving tissue engineering have contributed to regenerative medicine advances, to developed synthetic and natural scaffolds. To pursue these objectives, specific combinations of autologous or alogenous bone marrow progenitor cells seeded over a three-dimension scaffold with adequate properties, creating a hybrid material that can be modulated to patient reinsertion. In this review, information about synthetic and natural biological materials indicated to bone grats, combined or not with progenitor cells, aiming to stimulate bone healing process are presented.