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BACKGROUND: Regenerative endodontic therapy has the advantages of good biocompatibility, complete root development and high pulp vitality preservation rate. During vascular regeneration, scaffold is needed to maintain the survival of stem cells and vascular regeneration. OBJECTIVE: To analyze the effect of different bioactive scaffolds on regenerative pulp of young permanent teeth. METHODS: Totally 160 patients (168 teeth) with acute pulpitis at the age of 8 to 16 years, who were treated in the Second Hospital of Hebei Medical University from June 2016 to June 2018, were enrolled in this study. The patients were divided into traumatic group (n=92) and dental caries group (n=76) according to a cause of a disease. Each group was subdivided into mineral trioxide aggregate (MTA), iRoot BP Plus, autologous platelet-rich fibrin (PRF)+MTA and acellular dermal matrix (ADM)+MTA subgroups. The corresponding stents were used to treat regenerative pulp. At 1, 3, 6, 12 and 24 months, the tooth color, pulp vitality, and eruption height were recorded. Root canal length, root canal wall thickness, periapical shadow and apical foramen closure were compared using X-ray photography; and the success rate was calculated. This study was approved by the Ethics Committee of Second Hospital of Hebei Medical University. RESULTS AND CONCLUSION: (1) At the final follow-up in the traumatic group, tooth color, pulp vitality and eruption height were not significantly different among the four scaffold subgroups (P > 0.05). Root canal length, root canal wall thickness, periapical shadow and apical foramen occlusion were significantly different among the four scaffold subgroups (P 0.05). Root canal length, root canal wall thickness, periapical shadow and apical foramen occlusion were significantly different among the four scaffold subgroups (P < 0.05). Among them, the effect was best in the PRF+MTA subgroup. (3) No matter traumatic group or dental caries group, the success rate was significantly different among the four scaffold subgroups (P < 0.05); and the success rate was highest in the PRF+MTA subgroup. (4) Results verified that PRF combined with MTA has excellent biological induction in the treatment of young permanent teeth with regenerative pulp. Compared with other scaffolds, it can better preserve pulp vitality, induce the formation of young permanent teeth roots and improve the success rate of treatment.
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Objective To evaluate the quality of different parts of agarwood formed by stimulation with biological induction method, thus to provide evidence for agarwood quality control. Methods The identification of agarwood and the determination of alcohol extracts from agarwood were achieved by the methods of Chinese Pharmacopeia. The determination of total chromones from agarwood was achieved by ultraviolet spectrophotometry(UVS), with aquilarone E as reference substance and 760 nm as the detective wavelength. Results The UVS method was stable within 5 h and the recovery rate was 95.71%(sR=2.09%). The average alcohol extract content of the outside part of the xylem of Aquilaria sinensis(Lour.) Gilg(part B) was 109.4 mg/g,which was higher than that (100 mg/g) of the Chinese Pharmacopoeia standard. The average content of total chromones was 15.73 mg/g. The rest identification results were accorded with the requirements of Chinese Pharmacopeia. The average alcohol extract content of the inside part of the xylem(part Z) was 47.6 mg/g, and the average content of total chromones was 2.66 mg/g. The rest identification results did not reach the requirements of Chinese Pharmacopeia. Conclusion The obtained part B of the agarwood meets the requirements of Chinese Pharmacopeia, while part Z has color changed and its quality has not yet reached the requirements. The results indicated that the biological induction method impacts Aquilaria sinensis xylem from outside to inside, and then the xylem grows into agarwood gradually.
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10.3969/j.issn.2095-4344.2013.23.011