Influence factors of nucleoplasmic bridge frequencies in the peripheral blood lymphocytes of radiation workers / 中华放射医学与防护杂志

Objective@#To study the effects of sex, age, length of service, type of work and annual effective radiation dose on nucleoplasmic bridge (NPB) in the peripheral blood lymphocytes of radiation workers.@*Methods@#The peripheral blood samples of 100 radiation workers in Henan province were collected and the NPB in peripheral blood lymphocytes were measured by CBMN assay. The frequencies of NPB formation and NPB-containing cells were calculated, and the effects of various factors on NPB incidence were analyzed statistically.@*Results@#The NPB frequency in radiation workers was higher than that in healthy people (Z=-8.123, P<0.01). Except for sex, the factors of age, length of service, type of work and annual effective dose had significant influences on NPB (χ2= 7.202-45.571, P<0.05).@*Conclusions@#NPB reflects the effect of low-dose long-term chronic irradiation on the occupational radiation workers.

Fiber-reinforced composite resin bridges: an alternative method to treat root-fractured teeth

The replacement of missing teeth, especially in the anterior region, is an essential part of dental practice. Fiber-reinforced composite resin bridges are a conservative alternative to conventional fixed dental prostheses or implants. It is a minimally invasive, reversible technique that can be completed in a single visit. The two cases presented herein exemplify the treatment of root-fractured anterior teeth with a natural pontic immediately after extraction.

Influence factors of nucleoplasmic bridge frequencies in the peripheral blood lymphocytes of radiation workers / 中华放射医学与防护杂志

Objective To study the effects of sex,age,length of service,type of work and annual effective radiation dose on nucleoplasmic bridge (NPB) in the peripheral blood lymphocytes of radiation workers.Methods The peripheral blood samples of 100 radiation workers in Henan province were collected and the NPB in peripheral blood lymphocytes were measured by CBMN assay.The frequencies of NPB formation and NPB-containing cells were calculated,and the effects of various factors on NPB incidence were analyzed statistically.Results The NPB frequency in radiation workers was higher than that in healthy people (Z=-8.123,P＜0.01).Except for sex,the factors of age,length of service,type of work and annual effective dose had significant influences on NPB (x2=7.202-45.571,P＜0.05).Conclusions NPB reflects the effect of low-dose long-term chronic irradiation on the occupational radiation workers.

A Cadaveric Study Of Coronary Dominance And Prevalence Of Myocardial Bridges In South Indian Population

Background: Cardiovascular disease is one of the leading causes of morbidity and mortality in the world. Theaim of this study is to observe the branching pattern of left coronary artery with special attention to the originand termination of the posterior interventricular artery(PIVA), to determine the coronary dominance and theoccurrence of myocardial bridges over the anterior interventricular artery(AIVA). Myocardial bridging is acongenital anomalous condition in which a coronary artery takes a “tunneled” course under a “bridge” ofoverlying myocardium. The vessel is compressed in systole, resulting in angina, myocardial ischemia, acutecoronary syndrome, left ventricular dysfunction, sudden cardiac death and arrhythmias.Materials and Methods: This study was taken up at the Institute of Anatomy, Madurai Medical College with 60heart specimens collected from the cadavers of the same Institute and from the Department of Forensic Medicine.The heart specimens of different age groups, sex and socioeconomic status were preserved in 10% formalin andstudied by the conventional dissection method.Results: Among the 60 heart specimens studied, left coronary artery was seen bifurcating in 62%, trifurcating in32% and quadfurcating in 6%. Regarding dominance, right dominance with PIVA arising from right coronaryartery was seen in 53 specimens (88.3%), left dominance with PIVA being the continuation of left circumflexartery(LCX) was seen in 5 specimens(8.3%) and PIVA was arising from both coronaries in 2 specimens(3.4%).Among the 5 left dominant specimens, PIVA was seen as a continuation of LCX artery in 4 and single whereas in1 specimen the PIVA was seen to be double. With regard to termination of PIVA, it terminated in the middle ofposterior interventricular sulcus(PIVS) in 2 specimens and at the junction of anterior 1/3 and posterior 2/3 ofPIVS in the rest. Myocardial bridges were seen over anterior interventricular artery in 15 specimens (25%).Conclusion: Right dominance is seen in majority of hearts followed by left dominance and the balanced patternbeing the least. Presence of myocardial bridges was predominantly seen over AIVA.

Does Plan B work? Home range estimations from stored on board and transmitted data sets produced by GPS-telemetry in the Colombian Amazon / ¿Funciona el plan B? Estimación de rango de hogar a partir de datos almacenados en instrumentos a bordo de animales y los obtenidos a partir de telemetría satelital de GPS en el Amazonas colombiano

Abstract:Telemetry based on Global Positioning Systems (GPS) makes possible to gather large quantities of information in a very fine scale and work with species that were impossible to study in the past. When working with GPS telemetry, the option of storing data on board could be more desirable than the sole satellite transmitted data, due to the increase in the amount of locations available for analysis. Nonetheless, the uncertainty in the retrieving of the collar unit makes satellite-transmitted technologies something to take into account. Therefore, differences between store-on-board (SoB) and satellite-transmitted (IT) data sets need to be considered. Differences between SoB and IT data collected from two lowland tapirs (Tapirus terrestris), were explored by means of the calculation of home range areas by three different methods: the Minimum Convex Polygon (MCP), the Fixed Kernel Density Estimator (KDE) and the Brownian Bridges (BB). Results showed that SoB and IT data sets for the same individual were similar, with fix ranging from 63 % to 85 % respectively, and 16 m to 17 m horizontal errors. Depending on the total number of locations available for each individual, the home ranges estimated showed differences between 2.7 % and 79.3 %, for the 50 % probability contour and between 9.9 % and 61.8 % for the 95 % probability contour. These differences imply variations in the spatial coincidence of the estimated home ranges. We concluded that the use of IT data is not a good option for the estimation of home range areas if the collar settings have not been designed specifically for this use. Nonetheless, geographical representations of the IT based estimators could be of great help to identify areas of use, besides its assistance to locate the collar for its retrieval at the end of the field season and as a proximate backup when collars disappear. Rev. Biol. Trop. 64 (4): 1441-1450. Epub 2016 December 01.

Resumen:La telemetría basada en los sistemas de geopocisionamiento global (GPS) hace posible recopilar gran cantidad de información a escalas muy finas, y trabajar con especies imposibles de estudiar en el pasado. Al trabajar con telemetría de GPS, la opción de guardar información en la memoria interna del instrumento puede ser más deseable que sólo tener acceso a la información enviada vía satélite, debido a la mayor cantidad de localizaciones disponibles para analizar. No obstante, la incertidumbre de recuperar el collar hace que las tecnología de trasmisión vía satélite deba ser tenida en cuenta. Diferencias entre las bases de datos almacenadas en el collar (SoB) y las trasmitidas vía satélite (IT), recolectadas de dos individuos de Tapir de tierras bajas (Tapirus terrestris), son consideradas, en términos de las áreas de los rangos de hogar calculados con cada uno y mediante el uso de tres metodologías diferentes: Mínimo Polígono Convexo (MCP), Estimador de Densidad de Kernel Fijo (KDE) y los Puentes Brownianos (BB). Las bases de datos SoB e IT son similares, con tasas de acierto de localizaciones que oscilan entre 63 % to 85 % y errores horizontales de 16 m y 17 m respectivamente. Dependiendo del número total de localizaciones disponibles para cada individuo, los rangos de hogar estimados muestran diferencias entre 2.7 % y 79.3 %, para el contorno del 50 % de probabilidades, y entre 9.9 % y 61.8 % para el contorno del 95 % de probabilidades. Estas diferencias implican variaciones en la coincidencia espacial de los rangos de hogar estimados. Concluimos que el uso de la información trasmitida vía satélite no es una buena opción para la estimación de rangos de hogar, si la programción de los collares no ha sido diseñada específicamente para tal fin. Sin embargo, las representaciones geográficas de los estimados a partir de las bases de datos IT pueden ser de gran ayuda para la identificación de áreas de uso, además de su utilidad para la localización y recuperación de collares tras su liberación de los individuos monitoreados y como una base de datos de soporte en caso de pérdida del collar.

"Dynamic analysis on three indexes of biological dose estimation of the victim exposed to 192Ir radiation source at ""5.7"" accident in Nanjing" / 中华放射医学与防护杂志

Objective To explore the natural attenuation pattern of three biological dose estimation indexes in vivo by investigating the effect on biological dosimetry of peripheral blood sampling at different time points from the victim partially exposed to 192Ir radiation source at5.7 accident in Nanjing.Methods Peripheral blood of the patient was collected on days 5,40 and 280 after exposure,respectively.The yields of dicentrics plus rings chromosomes (dic + r),cytokinesis-block micronuclei (CBMN) and nucleoplasmic bridge + fusion + horse shoe + circular(NPB + FHC) were analyzed.The dynamic reduction and dose estimation were both observed using the biomarkers mentioned above after exposure.Results Compared to the estimates on days 5 after exposure,the dose values estimated on days 40 and 280 decreased by 34％ and 49％ fordic + r method,48％ and 79％ for the CBMN assay,and 48％ and 75％ for NPN + FHC method,respectively.Conclusions Three biological dose estimation indexes show a progressive decrease in vivo,with the half-life of dic + r/cell being 40 days.The doses estimated using these three indexes on days 40 after exposure showed a relative deviation more than 20％ compared with those on days 5 after exposure.

"Biological dosimetry for the victim accidentally exposed to 192Ir radiation source at ""5.7"" accident in Nanjing" / 中华放射医学与防护杂志

Objective To use three different methods in attempt to estimate the biological dose of the patient partially exposed to 192Ir source at5.7 accident in Nanjing,so as to provide dosimetric information for clinical remedy of exposed patients in the emergency of a nuclear accident.Methods Peripheral blood samples were collected on days 5 after exposure.The biological dose was estimated by the yields of dicentrics plus rings ( dic + r),cytokinesis-block micronuclei (CBMN) assay and nucleoplasmic bridge plus FHC (NPB + FHC).The homogeneity of radiation exposure was examined by Poisson distribution of dicentrics.Results By using three different methods,the whole body equivalent dose was dic + r estimated to be 1.51 Gy (95％ CI 1.40-1.61),1.47 Gy (95％ CI 1.36-1.60) by CBMN and 1.30 Gy (95％ CI 1.00-1.60) by NPB + FHC,respectively.A non-poisson distribution was also detected,suggesting partial body radiation exposure.Conclusions The estimated whole body equivalent dose ot a non-uniform radiation exposure was consistent with clinical diagnosis,suggesting that the yields ofdic + r,CBMN,as well as NPB + FHC,are efficient approaches to the estimation of biological doses.

A Rare Case with Absent Second Rib, Bifid Costal Cartilages and Bifid Xiphoid Process.

Thoracic cage is made up of twelve thoracic vertebrae, twelve pairs of ribs and costal cartilages (CC) and a sternum. Ribs articulate anteriorly with sternum through CC which are flattened bars of hyaline cartilage considered as unossified anterior part of embryonic cartilaginous ribs. Numerical and structural variations of ribs are well documented. In the present study, we observed both structural and numerical variations of thoracic cage, bilaterally. On both the sides, eleven pair of ribs were present, instead of seven, six true ribs were found and second rib with CC was absent. Second and third CC and third CC of right and third CC of left side were bifid. Cartilaginous bridges were present between various CC on both sides. In the midline xiphoid process was found to be bifid. These variations are of immense help to the physicians, surgeons and radiologists.

Entre mares e continentes: aspectos da trajetória científica de Hermann von Ihering, 1850-1930 / Between seas and continents: aspects of the scientific career of Hermann von Ihering, 1850-1930

Este artigo considera momentos da trajetória científica de Hermann von Ihering: sua formação em zoologia em instituições alemãs e em Nápoles, sua atuação internacional a partir do Brasil e retorno à Alemanha. Aborda aspectos da elaboração de suas teorias sobre pontes continentais. Destaca as redes de sociabilidade construídas prioritariamente com Florentino Ameghino e com emigrantes alemães como ele, que lhe permitiram interagir nos círculos científicos internacionais. Menciona fragmentos de suas cartas e de suas publicações nos períodos em que inicia a correspondência com Ameghino (1890); viaja à Europa em busca de apoio para suas teorias (1907); e publica um livro sobre a história do oceano Atlântico (1927).

This paper covers some periods in Hermann von Ihering’s scientific trajectory: his training in zoology in Germany and Naples, his international activities based in Brazil, and his return to Germany. It deals with aspects of the formulation of his theories on land bridges. It focuses on the network of contacts he maintained with German émigrés like himself, and primarily with Florentino Ameghino, which allowed him to interact in international scientific circles. It mentions excerpts of his letters and his publications in the periods when he began corresponding with Ameghino (1890), when he travelled to Europe in search of support for his theories (1907), and when he published his book on the history of the Atlantic Ocean (1927).

Cloning and synthesis of a new A-superfamily conotoxin Bt14.10 / 军事医学

Objective To clone a new conotoxin Bt14.10 from Conus betulinus derived from the South China Sea, synthesize the peptide , and to determine linkage of its disulfide bridges .Methods The genomic DNA was extracted from C.betulinus venom duct while the Bt14.10 sequence was cloned using primers designed based on the untranslated region and intron.The peptide was then synthesized using solid-phase method and folded into the target product whose disulfide bridge connection was further determined by two-step oxidative folding .Results A novel conotoxin designated as Bt 14.10 (CAHSVPGMHPCKCNNTC-NH2) was obtained,the disulfide connectivity of which was C1-C3,C2-C4.Conclusion Bt14.10 is a new A-superfamily conotoxin and has a distinct loop spacing pattern between cysteines in A-superfamily conotoxins.

Myocardial bridges and left coronary arterytrifurcation: a case report

During the performance of an angiotecnich in a human heart, to highlight the coronary circulation, weobserved the presence of myocardial bridges in the anterior and medial branches of the left coronary artery, inthis heart was also demonstrated the presence of an artery trifurcation left coronary branches that originatedthe anterior interventricular, circumflex and median. Myocardial bridges are intriguing entities that do notalways show signs and symptoms, the presence of the median artery in hearts with myocardial bridges, is oneof the factors that may explain the absence of signs and symptoms in some patients with this entity. Moreoverthe myocardial bridges can explain the signs and symptoms of ischemia on functional testing.

Motivos del desuso de puentes peatonales en Arequipa / Motives of disuse of pedestrian bridges in Arequipa

Objetivo: conocer las causas por las cuales las personas no utilizan los puentes peatonales de la ciudad de Arequipa. Métodos: estudio descriptivo realizado a través de la aplicación de una encuesta que recoge datos sobre la edad, género, grado de instrucción, procedencia y experiencia de accidentes previos. Se realizó un muestreo no probabilístico mediante la técnica de muestro por cuotas. Se pesquisaron 360 peatones, 270 hombres y 90 mujeres, que transitan por las vías aledañas a tres puentes de la ciudad: 1. El puente de la Avenida Venezuela, 2. El puente de la Avenida Alcides Carrión, y 3. El puente de la Avenida Ejército. Resultados: la edad promedio de la muestra era de 31,97 años y el grado de instrucción no fue determinante en el uso de los puentes. Se encontraron causas particulares para cada puente, lo que implica que su ubicación es relevante en la toma de decisiones de los peatones que los usan. La falta de tiempo y la pereza fueron los motivos más frecuentes que señalaron las personas para no utilizar los puentes peatonales. Conclusiones: es importante colocar bardas que eviten transitar por la pista indebidamente y realizar campañas de concientización que brinden información a los peatones sobre los riesgos a que se enfrentan por cruzar indebidamente la calle y por infringir la ley.

Objective: to find out the causes of disuse of pedestrian bridges in Arequipa city. Methods: descriptive study based on a survey gathering data on age, gender level of education, origin and experience of previous accidents. A non-probabilistic sampling was performed through quota sampling technique. Three hundred and sixty pedestrians (270 males and 90 females), who moved along pathways next to three city bridges such as Avenida Venezuela bridge, Avenida Alcides Carriion bridge and Avenida Ejercito bridge, were studied. Results: average age of the sample was 31.97 years, the level of education did not influence the use of bridges. Lack of time, sloth and the fear for attacks and robbery were the most frequent motives marked by pedestrians as causes for ignoring the pedestrian bridges. Conclusions: it is necessary to take measures in terms of security and surveillance at bridges, as well as launching awareness-raising campaigns that provide information to pedestrians on the possible risks of wrongly crossing the streets and of breaking the law.

Sucesso previsível como resultado do trabalho em equipe: reabilitação oral complexa usando cerâmica pura: parte 2 / Predictable success as a result of teamwork: complex full-mouth rehabilitation using all-ceramics: part 2

A escolha dos materiais e dos passos do tratamento exigidos somente deverá ser iniciada quando as metas finais do tratamento estiverem totalmente definidas. Desse modo, será possível obter-se sincronização entre eles, para possibilitar resultado com sucesso. Nesta segunda parte do artigo, os autores mostram um modo sistemático para a confecção de reabilitações orais em casos complexos. A primeira parte deste artigo tratou do eficiente planejamento e da seleção de materiais cerâmicos apropriados, esta segunda parte é dedicada aos procedimentos da fabricação e incorporação de restaurações extensas.

Only if the treatment goals have been well defined before the treatment is started may the range of materials and the required treatment steps be synchronized with each other and streamlined towards a successful outcome. In this two-part article Prof. Dr Daniel Edelhoff and Oliver Brix present a systematic way to the full-mouth rehabilitation of complex cases. The first part deals with the efficient planning and targeted selection of appropriate ceramic materials. This part is dedicated to the fabrication and incorporation of extensive restorations.

Sucesso previsível como resultado do trabalho em equipe: reabilitação oral complexa usando cerâmica pura: parte 1 / Predictable success as a result of teamwork: complex full-mouth rehabilitation using all-ceramics: part 1

A infinidade de materiais e as etapas de tratamento necessárias poderão ser sincronizadas e eficientes, no sentido de um desfecho de sucesso, somente se os objetivos do tratamento forem definidos antes de seu início. Neste artigo de duas partes, os autores apresentam um modo sistemático para a confecção de reabilitações orais em casos complexos. A primeira parte do artigo trata do eficiente planejamento e da seleção de materiais cerâmicos apropriados...

Only if the treatment goals have been well defined before the treatment is started may the range of materials and the required treatment steps be synchronized with each other and streamlined towards a successful outcome. In this two-part article Prof. Dr Daniel Edelhoff and Oliver Brix present a systematic way to the full-mouth rehabilitation of complex cases. The first part deals with the efficient planning and targeted selection of appropriate ceramic materials...

Morphometry and variations of bony ponticles of the atlas vertebrae (C1) in kenyans / Morfometría y variaciones de puentes óseos de la vértebra atlas (C1) en kenianos

Atlas bridges, the bony outgrowths over the third segment of the vertebral artery are associated with compression of the artery and nerves. There are limited studies comparing morphometry of the complete atlas bridges and that of the ipsilateral transverse foramen. Bilateral and gender differences in the morphometry of the complete bridges remain relatively unexplored. One hundred and two atlas vertebrae (49 male and 53 female) obtained from the Osteology Department of the National Museums of Kenya were used for this study. The presence of complete posterior atlas bridge (retroarticular canal) and lateral bridge (supratransverse foramen) was noted. Measurements were taken for the diameters of foramina, and the ipsilateral transverse foramina and their areas calculated. Complete posterior bridges occurred in 14.6 percent and 13.6 percent on the right and left sides respectively. The lateral bridge was found in 3.9 percent of the cases on the right side only. The complete posterior bridges had a cross-sectional area of 23.44mm2 on the right and 24.98mm2 on the left side. The lateral bridges had a mean cross-sectional area of 27.30mm2. The right and left transverse foramina had mean crosssectional area of 36.30mm2 and 37.20mm2 respectively, which was significantly larger than that of the ipsilateral complete and posterior bridges. The smaller dimensions of the complete atlas bridges compared to the ipsilateral transverse foramen suggest that they may predispose to vertebrobasilar insufficiency, Barre-Lieou and cervicogenic syndromes due to compression of the contents in the foramina.

Puentes del atlas, sobrecrecimientos óseos en el tercer segmento de la arteria vertebral se asocian con la compresión de la arteria y los nervios. Hay pocos estudios que comparan la morfometría de los puentes completos del atlas y el foramen transversal ipsilateral. Diferencias bilaterales y de género en la morfometría de los puentes completos permanecen relativamente inexplorados. Ciento dos vértebras atlas (49 hombres y 53 mujeres) obtenidos del Departamento de Osteología de los Museos Nacionales de Kenya se utilizaron para este estudio. Fueron identificados la presencia de puentes completos posteriores del atlas (canal retroarticular) y puentes laterales (foramen supratransverso). Se realizaron las mediciones de los diámetros del foramen y los forámenes transversos ipsilaterales, y se calcularon sus áreas. Puentes completos posteriores se observaron en el 14,6 por ciento y 13,6 por ciento de los lados derecho e izquierdo, respectivamente. El puente lateral se encontró en el 3,9 por ciento de los casos en el lado derecho solamente. Los puentes completos posteriores tuvieron un área transversal de 23,44mm2 en el lado derecho y 24,98mm2 en el lado izquierdo. Los puentes laterales tuvieron un área media de sección transversal de 27,30mm2. En los lados derecho e izquierdo se encontrron forámenes transversos con un área promedio de sección transversal de 36,30mm2 y 37,20mm2 respectivamente, lo que fue significativamente mayor que el de los puentes completos ipsilaterales y posteriores. Las dimensiones más pequeñas de los puentes atlas completos en comparación con los forámenes transversos ipsilaterales sugieren que pueden predisponer a la insuficiencia vertebrobasilar, síndromes de Barre-Lieou y cervical debido a la compresión de los contenidos en los forámenes.

Motivos de uso y no uso de puentes peatonales en la Ciudad de México: la perspectiva de los peatones / Use and non-use of pedestrian bridges in Mexico City: the pedestrian perspective

OBJETIVO. Analizar los motivos de uso y no uso de puentes peatonales (PP). MATERIAL Y MÉTODOS. Se empleó un diseño transversal, a partir de una muestra de peatones usuarios y no usuarios de PP; se utilizó regresión logística para identificar los factores que influyen en el uso y no uso de PP. RESULTADOS. La prevalencia de no uso fue 50.5 por ciento en 813 peatones entrevistados; la principal razón para usar PP fue "seguridad", del no uso fue "flojera". Hubo diferencias significativas al analizar motivos de no uso de PP en las edades de 19 a 36 años, ajustando por escolaridad y características físicas del PP, con RMa. 1.7 (IC95 por ciento 1.06-2.86) y RMa. 1.9 (IC95 por ciento 1.14-3.33), respectivamente. CONCLUSIONES. Los resultados de este estudio permiten identificar aspectos importantes a considerar desde la perspectiva de los peatones, antes de construir nuevos PP, así como aquéllos que deben mejorarse para incrementar su uso en zonas de alto riesgo de lesiones por atropellamiento.

OBJECTIVE. To analyze the motives for using and not using pedestrian bridges (PB). MATERIAL AND METHODS. A cross-sectional survey was conducted of a sample of pedestrian users and non-users of PB; a logistic regression model was used to analyze the motives for use and non-use. RESULTS. The prevalence of non-use was 50.5 percent of 813 surveyed pedestrians; the principal reason to use a PB was safety, and not to use it was "laziness". There were significant differences when analyzing the reason of non-use in the age groups 19 to 36 years, adjusted for education and physical characteristics of the PB ([aOR=1.7; 95 percent CI=1.06-2.86] and [ORa.1.9; 95 percent CI=1.14-3.33], respectively). CONCLUSIONS. The results of this study allow us to identify important aspects to consider "from the perspective of the pedestrians" when constructing new PB and improving existing PB to increase use in areas with a high risk of pedestrian injuries.

Comparison of Myocardial Bridges Imaging with Multi-slice Spiral CT and Coronary Angiography / 中国康复理论与实践

@#Objective To evaluate the clinical value of Multi-slice Spiral CT(MSCT) and coronary artery imaging in diagnosing coronary artery myocardial bridges (MBs). Methods Image data of 285 patients from July 2005 to July 2008 who accepted both the MSCT and coronary angiography CAG at our hospital were collected. Diagnostic performance of the two Methods were analysed and the diagnostic accuracy were compared. Results In 285 cases of patients with MSCT examinations, 82 coronary artery were diagnosed in 59 cases, the detection rate of which was 20.7%. In contrast, 16 cases were diagnosed by CAG and the detection rate was 5.6%. In the 82 coronary artery diagnosed by MSCT, 42 coronary artery were single vessel, the proportion was 71.19%. Others were two vessels or three vessels. There were 67 in left anterior descending artery (LAD),in which 52 were near the middle and 15 were distal. 26 MBs diagnosed by MSCT were included in the 28 MBs diagnosed by CAG. Put CAG as the gold standard of myocardial bridge detecting, then MSCT's sensitivity was 92.86%. Conclusion MSCT coronary artery imaging can accurately show the anatomical relationship between coronary artery and myocardial and it is one of the preferred method of diagnosing coronary artery.

Efficacy of HUMN criteria for scoring the micronucleus assay in human lymphocytes exposed to a low concentration of p, p'-DDT

The cytokinesis-block micronucleus (CBMN) assay is one of the standard cytogenetic tools employed to assess chromosomal damage subsequent to exposure to genotoxic/cytotoxic agents, and is widely applicable to plant, animal and human cells. In the present study, the CBMN assay was used to assess the baseline damage in binuclear human peripheral blood lymphocytes exposed to 25 µg/L p,p'-DDT for 1, 2, 24, and 48 h by measuring the frequency of micronuclei, nucleoplasmic bridges and nuclear buds. These new scoring criteria facilitated the detection of different types of clastogenic and aneugenic effects induced by this type of pollutant. With these criteria, CBMN can also be used to measure nucleoplasmic bridges which are considered to be consequences of chromosome rearrangements and nuclear buds which are biomarkers of altered gene amplification and gene dosage. The total number of micronuclei observed in binuclear human peripheral blood lymphocytes of the exposed samples (ranging from 32 to 47) was significantly greater (P < 0.05) than that detected in the unexposed (0 time) control sample, where the total number of micronuclei was 7. The number of nucleoplasmic bridges and nuclear buds obtained after 24 and 48 h was also significantly (P < 0.05) greater in the samples treated with p,p'-DDT than in the unexposed control samples. Thus, our results confirmed the usefulness of the new criteria applicable for the CBMN assay employed in measuring the DNA damage and its role of a sensitive cytogenetic biomarker.

Inestabilidad cariológica durante la formación de células madres del polen en Aloe vera (Aloaceae)

Con el objetivo de esclarecer la posible existencia de anomalías citogenéticas que aminoren la fertilidad del polen de Aloe vera, se analizó la etapa de proliferación celular que lleva a la formación de células madres del polen (CMPs). Se recolectaron botones florales (BF) en 25 plantas de una población ubicada a 10°34’15’’ N, 64°12’08’’ W, los cuales fueron fijados en Carnoy I por 24 h y almacenados en etanol (70 % v/v). Las observaciones se realizaron en preparaciones temporales obtenidas por la tinción del contenido de las anteras suspendidas en orceína acética (1.5 % p/v) por 5 minutos. De las 9 411 células analizadas, 17 % mostraron 1-8 puentes entre cromátidas hermanas, 13 % 1-7 micronúcleos de 0.9-4.8 µm, 8.1 % estaban unidas por puentes y 0.1 % no contenían cromatina. El resto de las células (61.8 %) presentó configuraciones aparentemente normales y sin variaciones morfométricas. La proliferación irregular de una fracción de CMPs (39.2 %) sugiere que las condiciones ambientales de la zona árida donde se realizaron los muestreos inducen inestabilidad cromosómica y cambios fisiológicos que afectan el normal desarrollo de la mitosis premeiótica, generando pérdida o adición de fragmentos, asociados a deficiencias y duplicaciones génicas.

4 Cases of Mucosal Bridges in the Esophagus / 대한소화기내시경학회지

Mucosal bridge, endoscopically observed, is a cord-like mucosal connection across the lumen. The bridge is very elastic and stretches easily, unlike granulation tissue. Mucosal bridges of the esophagus have been occasionally described in various circumstances, particularly in congenital or acquired origin as the inflammatory diseases. The occurrence of mucosal bridges due to inflammatory process may arise anywhere from the esophagus to the colon. It has been more frequently reported in the colon than in the esophagus, stomach, and duodenum. We experienced four cases of esophageal mucosal bridges and three of them were accompanied by esophageal diverticulum. We report these cases with a review of relevant literatures.