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Objective To analyze the enzyme kinetics of active ingredient of Buddleja lindleyana(AIBL)against Oncomela?nia hupensis,the intermediate host of Schistosoma japonicum. Methods O. hupensis snails were placed in 1 000 ml of 3.55 mg/L AIBL solution for 24,48 h and 72 h,respectively,and the enzyme kinetics of alanine aminotransferase(GPT)was deter?mined by Reitman?Frankel assay,lactate dehydrogenase(LDH)by the chemical inhibition lactic acid substrate method,alka?line phosphatase(AKP)by the disodium phenyl phosphate colorimetric method,acetylcholine esterase(AChE)and malate de?hydrogenas(MDH)by ELISA,and succinate dehydrogenase(SDH)by the phenazine methyl sulfate reaction method(PMS)in the soft tissues of O. hupensis before and after AIBL treatment. Results Following exposure to 3.55 mg/L AIBL solution for 24 h, the GPT,LDH,and AKP activities significantly improved in the soft tissues of O. hupensis,while the SDH and MDH activities were significantly lowered in the head?foot and liver. However,AIBL treatment did not cause significant effect on AChE activity in O. hupensis. Conclusions AIBL causes significant damages to O. hupensis liver and can efficiently act on anaerobic and aer?obic respiration loci,which will hinder energy metabolism,and cause inadequate energy supply in cells used for normal secre?tion,eventually leading to O. hupensis death.
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To establish an HPLC-ELSD method for the quantification of triterpenoids in the fruits of Buddleja lindleyana. The RP-HPLC-ELSD method was used for the determination of triterpenoids in B. lindleyana fruits, which were collected from different habitats. The column used was a packed with 5 μm stationary phase Waters SunFireTM C₁₈ (4.6 mm×150 mm, 5 μm). The mobile phase consisted of Methanol-water(82∶18) at a flow rate of 1 mL•min⁻¹. Column temperature： 30 ℃. ELSD conditions： drift tube temperature： 106 ℃; carrier gas (nitrogen) flow rate： 1.5 L•min⁻¹; amplification factor： 1. The calibration curves showed good linear relationship on a range from 0.702 to 28.08 μg(r=0.999 2) for Clinoposaponin III, 0.390 to 15.60 μg(r=0.998 9) for Desrhamnoverbascosaponin and 0.192 to 7.68μg(r=0.999 0) for Mimengoside I. The average recovery rate(n=6) were 99.41%, 99.08% and 98.67% and it's RSD were 0.86%, 1.56% and 1.80%. This method can be used to determine the contents of triterpenoids in the fruits of Buddleja lindleyana for its simplicity, accurateness and reliability.
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Objective To investigate the anti-H5N1 activities and chemical constituents from stems and leaves of Buddleja lindleyana Fort. Methods Constituents were separated through AB-8 macroporous resin, chromatography of silica gel, Sephadex LH-20 and recrystallization. Structures of the compounds were identified by analysis of physicochemical properties and spectral data. Results Ten compounds were isolated and identified as linarin(l), rutin(2), luteolin(3), quercetin(4), apigenin(5), hesperetin (6), salidroside (7), oleanolic acid (8), ß-sitosterol (9), and daucosterol (l0), respectively. Conclusion Compounds 2-7 were obtained from this plant for the first time.
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Objective To investigate the anti-H5N1 activities and chemical constituents from stems and leaves of Buddleja lindleyana Fort.. Methods Constituents were separated through AB-8 macroporous resin, chromatography of silica gel, Sephadex LH-20 and recrystallization. Structures of the compounds were identified by analysis of physicochemical properties and spectral data. Results Ten compounds were isolated and identified as linarin(1), rutin(2), luteolin(3), quercetin(4), apigenin(5), hesperetin (6), salidroside (7), oleanolic acid (8),β-sitosterol (9), and daucosterol (10), respectively. Conclusion Compounds 2-7 were obtained from this plant for the first time.