Comparison between the concentration of mast cells and risk criteria of malignancy in intestinal adenomas

Intestinal adenomas are benign neoplasms that present a risk of malignancy associated with three independent characteristics: the polyp size, the histological architecture and the severity of epithelial dysplasia (or atypia). Current evidence suggests that mast cells (CM) contribute to the tumorigenesis of colorectal carcinomas. Objective: Compare the concentration of CM in intestinal adenomas and risk criteria for malignancy in these tumors (size, histological type and degree of cellular atypia). METHODS: We conducted a retrospective study with 102 anatomopathological reports of intestinal adenoma excision. We selected paraffin blocks with the central area of the tumor. The CM were stained with toluidine blue. RESULTS: In most cases (89.2%, n=91), the mast cells concentration (MC) was less than 6 CM/10 high power field (HPF) (p=0.0001). Most adenomas, regardless of their histological type, showed 0 CM/10 HPF (p=0.083). In most adenomas, regardless of their size, MC was 0 CM/10 HPF (p=0.665). Presence or absence of atypia was associated, in most cases, with MC of 0 CM/10 HPF (p=0.524). Conclusion: This study did not show association between the MC and histological type, size or presence of atypical cells in intestinal adenomas. (AU)

Adenomas intestinais são neoplasias benignas que apresentam risco de malignização relacionado a três características independentes: o tamanho do pólipo, a arquitetura histológica e a gravidade da displasia (ou atipia) epitelial. Evidências atuais sugerem que os mastócitos contribuem para a tumorigênese do carcinoma colorretal. OBJETIVO: Analisar comparativamente a concentração de mastócitos em adenomas intestinais e os critérios de risco para malignização nesses tumores (tamanho, tipo histológico e grau de atipia celular). Métodos: Realizou-se um estudo retrospectivo, com seleção de 102 laudos anatomopatológicos de exérese de adenoma intestinal. Foram selecionados os blocos de parafina com a área central da neoplasia para a realização da coloração de azul de toluidina para evidenciar os mastócitos. RESULTADOS: Na maioria dos casos (89,2%, n=91) a concentração de mastócitos (CM) foi menor que 6 mastócitos/10 campos de grande aumento (CGA) (p=0,0001). A maioria dos adenomas, independente do tipo histológico, mostrou 0 mastócito/10 CGA (p=0,083). A maioria dos adenomas, independentemente do tamanho, tinha CM de 0 mastócito/10 CGA (p=0,665). A presença ou a ausência de atipias esteve associada, na maioria dos casos, a CM de 0 mastócito/10 CGA (p=0,524). CONCLUSÃO: Este estudo não mostrou associação entre a concentração de mastócitos e tipo histológico, tamanho ou presença de atipias celulares nos adenomas intestinais. (AU)

IMMUNOHISTOCHEMICAL STUDIES ON GASTRIC CANCER AND INTESTINAL METAPLASIA BY MONOCLONAL ANTIBODY MC3 / 解放军医学杂志

The immunohistochemical reactivity of MC3,a monoclonal antibody (MoAb) against human colonic cancer,was studied in 110 specimens with gastric cancer (GC) and 343 specimens with intestinal metaplasia (IM) taken from different diseases,including GC (n=79),dysplasia (n=32),chronic atrophic gastritis (CAG,n=173),gastric ulcer(GU,n=28) and chronic superficial gastritis (CSG,n=31).IM was subtyped into three types:complete (type I) and two classes of incomplete IM (type II and type III) depending on the absence or presence of sulphomucin within the mucin secreting columnar cells.The positive rate of staining with MoAb MC3 in GC was 68.2%.The positive reactivity of MC3 was significantly more common in IM specimens with GC and in those with dysplasia than in those with benign gastric pathology.The positive rate of staining with MC3 in type III IM (48.6%) was significantly higher than those in type I (19.9%) and type II IM (28.0%) (P