RESUMEN
Objective:To investigate the long-term outcomes of laparoscopic ventral rectopexy (LVR) for obstructive defecation with overt pelvic structural abnormalities.Methods:The retrospective cohort study was conducted. The clinical data of 31 obstructive defecation patients with overt pelvic structural abnormalities who were admitted to the Renji Hospital of Shanghai Jiaotong University School of Medicine from June 2014 to August 2020 were collected. There were 6 males and 25 females, aged 59(range, 32?81)years. All 31 patients underwent LVR through transabdominal approach. Observation indicators: (1) the Cleveland clinic constipation score (CCCS); (2) severity of obstructive defecation; (3) patients assessment of constipation quality of life (PAC-QoL). Follow-up was conducted using telephone interview and outpatient examination up to October 2021. One professional researcher assessed the constipation symptoms and quality of life of patients through outpatient interview or mobile software platform of Questionnaire Star. Measurement data with skewed distribution were represented as M(range), and comparison before and after operation was conducted using the Wilcoxon sign rank test. Results:(1) The CCCS. All 31 patients underwent LVR for the first time and were followed up for 61.8(range, 11.0?87.0)months. The constipation symptoms of the 22 patients were improved. The CCCS of the 31 patients before surgery and at the last follow-up time were 15.8(range, 8.0?26.0) and 10.7(range, 2.0?20.0), respectively, showing a significant difference ( Z=?3.98, P<0.05). (2) Severity of obstructive defecation. The severity scores of frequency of bowel movements, difficult of bowel movements, sensation of incomplete defecation, abdominal distension or pain, time of each bowel movements, daily unsuccessful times of defecation, artificial assisted defecation for the 31 patients were 2.9(range, 1.0?4.0), 3.0(range, 1.0?4.0), 1.9(range, 0?3.0), 0.5(range, 0?3.0), 2.6(range, 2.0?4.0), 2.0(range, 0?4.0), 0.9 (range, 0?2.0) before surgery, versus 1.7(range, 0?4.0), 1.6(range, 0?4.0), 1.2(range, 0?4.0), 0.3(range, 0?3.0), 1.7(range, 0?3.0), 1.4(range, 0?3.0), 0.7(range, 0?2.0) after surgery, respectively. There were significant differences in the frequency of bowel movements, difficult of bowel movements, sensation of in-complete defecation, abdominal distension or pain, time of each bowel movements, daily unsuccessful times of defecation for the 31 patients before and after surgery ( Z=?3.38, ?3.80, ?2.54, ?2.31, ?3.64, ?2.75, P<0.05) and there was no significant difference in the artificial assisted defecation for the 31 patients before and after surgery ( Z=?1.31, P>0.05). (3) PAC-QoL. The score of physical discomfort, satisfaction, worries and concerns, psychological discomfort for the 31 patients were 2.3(range, 1.0?4.0), 3.2(range, 1.0?4.8), 2.2(range, 0.6?4.0), 1.8(range, 0.4?3.9) before surgery, versus 1.6(range, 0?4.0), 2.3(range, 0?4.0), 1.7(range, 0?4.0), 1.3(range, 0?4.0)after surgery, respectively, showing significant differences before and after surgery ( Z=?3.49, ?2.17, ?2.50, ?3.05, P<0.05). Conclusions:The long-term outcomes of LVR for obstructive defecation with overt pelvic structural abnorma-lities are satisfactory. Symptoms as frequency of bowel movements, difficult of bowel movements, sensation of incomplete defecation, abdominal distension or pain, time of each bowel movements and daily unsuccessful times of defecation will be significantly improved after LVR and the constipation quality of life of patients will be improved.
RESUMEN
Objective To investigate the effects of phonosurgery combined with botulinumtoxinof type A in-jection in adults with vocal nodules .Methods A total of 76 adults with diagnosis of vocal nodules were studied in this experiment .They were divided into two group according to the different treatments :the surgery group (n=40) ,and the group with the combined botulinum toxin of type A injection (n=36) .Preoperative and postoperative examinations consisted of electronic laryngoscope ,stroboscopic laryngoscope ,voice assessments of subjective and objective evaluations using GRBAS classification and voice disorder index (VHI) .The evaluation parameters were the longest phonation time (MPT) ,jitter and shimmer and dysphonia severity index (DSI) .The results from the two groups were compared .Results There were 5 cases cured ,28 cases improved and 7 cases of now effects in the surgery group .The effective rate was 82 .5％ .There were 30 cases cured and 6 cases improved in the combined in-jection group with the effective rate of 100％ .There were 22 cases with good glottis closure and 18 cases with incom-plete closure in the surgery group postoperatively ;in the combined injection group ,30 cases glottis closed well and 6 cases still closed incompletely .The differences of postoperative VHI ,MPT ,jitter ,shimmer and DSI in addition to G were significant different between the two groups .Conclusion Phonosurgery combined with botulinum toxin of type A injection is an effective way for the treatment of vocal fold nodules in adults .
RESUMEN
Aim To observe the influences of dexmen-detomidine on the spontaneous contraction of duodenal smooth muscle of rabbits in vitro and explore the mech-anisms.Methods The rabbits ( male or female ) were stunned and the duodenums were isolated .The sam-ples of duodenal segments were connected with tension transducer , which were then put into oxygen saturation Krebs-Henseleit ( K-H) solution .The influences of dex-mendetomidine on amplitude ( AM ) and frequency ( FR ) of duodenal smooth muscle were recorded by BL-420 F biological signal processing system .The cu-mulative dosing method was used to observe the differ-ent concentrations of dexmedetomidine on duodenal smooth muscle spontaneous contraction .Glibenclamide ( Gli) was added to K-H solution before dexmendeto-midine.In the calcium-free K-H solution, calcium chloride and rynodine were added before dexmendeto-midine.The mechanisms of dexmendetomidine were studied .Results ① Dexmendetomidine reduced the amplitude of spontaneous contraction of duodenal smooth muscle in rabbits in a dose-dependent manner ( P0.05 ) .② Gli ( P <0.05 ) partly abolished the inhibitory effects of dexmendetomi-dine on duodenal smooth muscle .③ Dexmendetomi-dine inhibited the contraction of duodenum smooth muscle induced by calcium chloride ( P <0.05 ) and rynodine ( P<0.05 ) application into calcium-free K-H solution.Conclusion Dexmendetomidine inhibits the spontaneous contraction of duodenal smooth muscle of rabbits in vitro.The mechanisms may be related to ac-tivating ATP sensitive potassium channels , inhibition of the extracellular calcium influx via cell membrane and intracellular calcium release via sarcoplasmic reticulum in duodenal smooth muscle .
RESUMEN
Objective To make a preliminary study on the results from qualitative and quantitative detection of low concentra-tion hepatitis B surface antigen(HBsAg).Methods 85 HBsAg low concentration serum samples (ELISA method test re-sults were 0.60.05)and 0.60.05).The positive detection rate showed statistically difference between ELISA (70.6%)and CMIA,TRFIA in 0.6TRFIA>ELISA.②The contents of HBsAg showed statistically difference among 0.6TRFIA>ELISA.③There was a positive correlation between the three methods of HBsAg content (t=2.939,2.928,60.915,P<0.05).The correlation between CMIA method and TRFIA method was the best (r=0.989).Con-clusion CMIA was the first choice for testing low concentration HBsAg,TRFIA was the second.For the specimens of the low concentration HBsAg detected by ELISA should be suggested to clinical and retested by CMIA or TRFIA in order to a-void missing detection.And it was not recommended to clinical that different methods of quantitative or half-quantitative re-sults were transverse compared in order to avoid misdiagnosis.
RESUMEN
PURPOSE: To assess clinical outcome of clear lens extraction in high myopia after epikeratophakic (EKP) lenticule removal and to evaluate the change of cornea in EKP. METHODS: The retrospective study reports 5 eyes of 3 patients who underwent clear lens extraction (CLE) after EKP lenticule removal to treat myopia. The changes in best corrected and uncorrected visual acuity, spherical equivalent and corneal refractivity were evaluated before and after EKP, EKP lenticule removal and CLE. RESULTS: The cornea was clear, but the refractive power and astigmatism of cornea increased after EKP lenticule removal. The degree of change of the cornea seemed to be associated with the period between the EKP lenticule removal and the CLE. The BCVA in 3 of 5 eyes returned to the pre-EKP level after CLE. The eye that underwent CLE without EKP lenticule removal revealed severe overcorrected hyperopia and was corrected after CLE. The eye that had CLE without the EKP lenticule removal showed the overcorrection postoperatively but it was corrected after the EKP lenticule removal. CONCLUSIONS: The CLE following EKP lenticule removal can be an effective refractive surgery in complicated EKP patients. To obtain the accurate refractive estimate postoperatively, it is better to perform EKP lenticule removal preceding CLE.
Asunto(s)
Humanos , Astigmatismo , Córnea , Hiperopía , Miopía , Procedimientos Quirúrgicos Refractivos , Estudios Retrospectivos , Agudeza VisualRESUMEN
To study the inhibitory effects of laminarin (acidic polysaccharide) J201A on the proliferation of human lung fibroblasts (HLF) and its related mechanism of action. Methods: Effect of J201A on the proliferation of HLF was evaluated by MTT assay, and the effects on cell cycle and synthesis of proteins of HLF were assessed by flow cytometry. The existence of J201A receptors on HLF was confirmed by fluorescent staining assay. Results:J201 A inhibited the synthesis of proteins and the proliferation of HLF at G0/G1 stage of cell cycle and J201A receptors existed on HLF. Conclusion:J201A exerted its antifibrotic activity by inhibiting HLF at G0/G1 stage of cell cycle and the synthesis of proteins.
RESUMEN
AIMTo investigate whether DADS induce MGC803 cell apop tosis and cell cycle arrest. METHODSMGC803 cell growth inhibitio n was measured by MTT assay. Flow cytometry and acridine orange fluorescent stai ning method were used to determine the induction of apoptosis and the change of cell cycle. RESULTSMTT assay showed that adding 20,30,40 mg?L -1 DADS for 72 h suppressed MGC803 growth by 25 7%,58 6%,69 0% respective ly. Partial cells presented the characteristic morphological changes of apoptosi s under the fluorescent microscope. The apoptosis rate ncreased in time-depende nt manner. Flow cytometry analysis revealed that treating MGC803 cell with DADS significantly increased in the percentage of cells in the G 2/M phase. The proportion of cells in the G 2/M phase after treatment with 30 mg?L -1 DADS for 24 hours was comparable (46 0%), and more than four times that occu rring in untreated cells (9 9%). Furthermore, flow cytometry analysis also demonstrated that DADS induced apoptosis of MGC803 cell in time-dependent manner. T he pencentage of apoptotic cell was 3 53% after 0 h of 30 mg?L -1 DADS tr eatment. This pencentage of apoptotic cell rose steadily over time reaching 9 8 % after 24 h and 39 5% after 48 h. CONCLUSIONDADS could induce apoptosis of MGC803 cells and block the cell cycle at G 2/M phase.
RESUMEN
Objective To explore the influence of hypoxia on cystathionine gamma lyase (CSE) gene expr ession of rat cultured pulmonary artery smooth muscle cells (PASMCs) and the mod ulatory effect of hydrogen sulfide on PASMCs proliferation.Methods Rat′s cultured PASMCs were randomly divided into contr ol group,NaHS group,hypoxia group and hypoxia +NaHS group. Each group contains 6 wells of cells. Hypoxic challenge was performed with oxygen concentr ation≤1%. The cell proliferation rate was measured by 3H-thymidine i ncorporation into DNA of the PASMCs. The expression of CSE mRNA was measured by quantitative competitive reverse-transcription polymerase chain reaction (RT-PCR ).Results For the PASMCs in hypoxia group, the amount of CSE mRN A was down-regulated about 67% (P
RESUMEN
Chilblain lupus erythematosus(CLE) is a chronic unremitting from of LE seen predomin antly in women, The chilblain lesion occurs commonly on the digits, calves and heels. Chronic facial discoid LE usually appears before the chilblain form. A 23-year-old female had relatively well defined, erythematous discoid plaques on her both cheeks and scattered erythema multiforme-like ring lesions on her right hand and right wrist. There were also chilblain lesions showing multiple, purple colored macules on her knees, lower legs and periungual areas of fingers and toes. The skin lesions developed at November, 1982 and then the skin lesions remitted during the next summer. The skin lesions recurred at December, 1983.