RESUMEN
To study the effect of in vitro fertilization-embryo transfer (IVF-ET) on the contractile function of mesenteric vessels in offspring mouse model and its regulatory mechanism. Methods Offspring mouse model of IVF-ET (20 weeks after birth) was built to compare with natural born descendant in vascular regulation. The bodyweight and BMI of mice were measured. Serum levels of cardiovascular related cytokines were tested by ELISA and colorimetric method. Samples of mesenteric vascular were from IVF-ET mice and normal controls. Given the vital function in regulating vascular contraction which Ca2+signaling pathway exhibited , four representative genes (Calm2, Itprl, RyR2, RyR3) were selectedfor study. And the mRNA expression levels of Calm2, Itpr2, RyR2, RyR3 were tested by qPCR. The protein expression levels of Calm2 and Itprl were tested by Western blot. Results Mice born under IVF-ET showed increased bodyweight and abnormal BMI after 20th week, and the serum levels of NO and Ang II were significantly higher than those of control (P < 0. 0 5) . The expression levels of Calm2, Itprl were up-regulated both in molecular and protein levels (P <0. 0 1) , RyR3 was up-regulated in molecular level (P < 0. 01) , while RyR2 was down-regulated in molecular level (P < 0. 0 1) . Conclusions The changes of serological markers and regulatory gene expression level related to vasoconstriction function may be closely related to the increased risk of cardiovascular diseases in IVF-ET offspring.
RESUMEN
OBJECTIVE: To observe the blood-brain barrier permeability of scutellarin ethyl ester, the structural modification product of scutellarin, and to explore the mechanism of nerve protection on cerebral ischemia. METHODS: By co-culturing brain microvascular endothelial cells (BMVEC) and astrocytes cells (AC) in vitro, blood-brain barrier model was established. The active components were analyzed by HPLC. Rat cerebral cortical neurons cells were cultivated in vitro, and the survival rate was determined by MTT assay. The model of rat cortical neurons cell injury was established by glutamate and KCl, and the change of fluorescence intensity was detected by Laser Scanning Confocal Microscopy (LSCM). RESULTS: Compared with control group, scutellarin ethyl ester could penetrate BBB, promote the survival of cortex nerve cells in vitro significantly(P < 0.01), and inhibit intracellular calcium overload induced by 500 μmol · L-1 glutamate (P < 0.01). CONCLUSION: Scutellarin ethyl ester can pass through BBB and has neuroprotective effect by regulation of calcium signal pathway to resist cerebral ischemia. Copyright 2012 by the Chinese Pharmaceutical Association.