RESUMEN
Objective To investigate the chemical constituents in the leaves of Camellia chrysantha. Methods The chemical constituents were separated and purified by silica gel, Sephadex LH-20 column chromatography, recrystallization, preparative HPLC and so on. Their structures were determined by physical constants and spectral analyses. Results Ten compounds were isolated and identified as 3,4-methylenedioxy-3′-O-methyl-4′-O-(6′-O-acetyl-glucoside) ellagic acid (1), oleanolic acid (2), α-spinasteryl-3- O-β-D-glycoside (3), n-dotriacontanol (4), okicamelliaside (5), 6-hydroxy-kaempferol-3-O-β-D-glucopyranoside (6), 3,4-O,O- methylidyne-ellagic acid (7), ellagic acid-4-O-β-D-glucopyranoside (8), 3,4-methylenedioxy-3'-O-methyl-4'-O-glucoside ellagic acid (9), and p-(hydroxymethyl) phenol 5-O-β-D-glucopyranosyl (1→2)-β-D-(4-O-p-coumaryl)-glucopyranoside (10). Conclusion Compound 1 is a new ellagic acid named camellia A. Compounds 4—10 are obtained from this plant for the first time.
RESUMEN
Objective Using a short-term in vivo laboratory model of murine liver cancer induced with diethylnitrosamine (DEN),we inspected the impact of 5% C.Chrysantha leaves and 5% concentrations on induction of liver cancer with DEN.Methods Laboratory Winstar rats,all being male and SPF grade,were administered with the liver cancer initiator DEN(200?g/kg,ip,once only),then with murine food containing 0.015% 2-AAF (2-Acetaminoflueren) for 2 weeks,following an excision of the augmented parts as a selective promoting program that speeded up the proliferation of precancerous foci from the initiated hepatocytes.On the beginning of this experiment,5% C.Chrysantha leaves and 5% concentration-contained food were given to the trail rats; in contrast,normal food to the control.Rats were killed 3 days after feeding of 2-AAF-contained food.Targeting part of liver were collected for ?-glutamyltranspeptidase (?-GT) staining,and numbers and areas of?-GT positive foci per cm2 of the liver area were calculated using a software designed by us.Results Numbers and areas of?-GT positive foci of the trail are significantly less than those of the control.Conclusion 5% C.Chrysantha leaves and 5% concentrations inhibit the DEN induction to liver cancer.