RESUMEN
Capsosomes, a new class of polymer capsules containing liposomes subcompartments, which preserve the advantages of both polyelectrolyte microcapsules and liposomes while eliminating some of the shortcomings, are formed through the layer-by-layer (LBL) assembly of polymers and liposomes and expected to find diverse applications in biomedicine, especially for the creation of artificial cells or organelles. In this review, the design and performance of capsosomes are mainly introduced, and capsosomes are pointed out to be a promising platform toward the creation of therapeutic artificial cells and organelles or as a novel approach for multiple drug delivery.
RESUMEN
OBJECTIVE: To study the tissue distribution of capsosomes in vivo and the efficacy of DOX-loaded capsosomes in vitro. METHODS: Cy5-Capsosomes (capsosomes encapsulating Cy5-BSA molecules) were prepared by co-precipitation method and layer-by-layer assembly technique. After intravenously injected into nude mice via the tail vein, the tissue distribution of Cy5-capsosomes at defined time points were studied under Maestro in-vivo imaging system. The cytotoxicity of DOX-loaded capsosomes for B16F10 cells was identified by MTT, the apoptosis induced by DOX-loaded capsosomes was observed by means of fluorescent microscopy. RESULTS: Cy5-Capsosomes achieved in the lung less than 1h and accumulated there for more than 12h. There was little Cy5-Capsosomes distributed in other organs. Capsosomes had low toxicity, while the DOX-loaded capsosomes had strong toxicity for B16F10 cells and induced apoptosis obviously. CONCLUSION: The capsosome had favourable passive pulmonary targetability, and it can be used as a novel drug carrier in potential.
RESUMEN
OBJECTIVE: To fabricate a novel sandwich-type drug carrier called "capsosome" using natural polyelectrolytes such as chitosan and alginate, then characterize the structure, drug loading and release profile. METHODS: Chitosan and alginate and DMPG (Dimyristoyl Phosphatidylglycerole) liposomes were coated on the CaCO3 microparticles using layer-by-layer assembly technique. Upon removal of the CaCO3 template core, stable capsosomes, containing one layer of intact liposomes as cargo, were obtained; As a model drug, DOX with different concentrations was used to evaluate the drug loading ability of capsosome, the cumulative release a-mount was determined at different time points. RESULTS: Confocal laser scanning microscope images and transmission electron microscope images indicated that liposomes were embeded in the microcapsule shell successfully, drug loading amount of the obtained capsosomes increased with the DOX incubation concentration increasing (100-1000 μg · mL-1), the highest loading amount could reach to 30.47 × 10-3 ng/each capsosome, the cumulative release amount was about 46% in 48 h. CONCLUSION: The capsosome had favourable drug loading and release ability, it deserved to be studied as a novel drug carrier. Copyright 2012 by the Chinese Pharmaceutical Association.