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Achyranthes bidentata Blume is widely used as a traditional Chinese medicine with the effects of nourishing the liver and kidneys and strengthening muscles and bones. In this work, a rapid and simple strategy was developed for characterizing phytoecdysteroids by ultra-high-performance liquid chromatography coupled with liner ion trap-Orbitrap mass spectrometry using electrospray ionization in the negative mode. As a result, 47 phytoecdysteroids were unambiguously or tentatively characterized. Among them, seven known compounds were identified according to the reference standards along with molecular formula, retention time and fragmentation patterns, while others were mostly potential new compounds. Through targeted isolation, the structures of three new compounds were determined by NMR spectra, which were consistent with LC-MS characterization. The present study provides an efficient method to deeply characterize phytoecdysteroids.
Asunto(s)
Achyranthes/química , Cromatografía Líquida de Alta Presión/métodos , Cromatografía de Gases y Espectrometría de Masas , Espectrometría de Masas , Medicina Tradicional China , Espectrometría de Masa por Ionización de Electrospray/métodosRESUMEN
Objective: A novel and generally applicable approach was established for the interaction analysis on compatibility of Danshen-Honghua (Salviae Miltiorrhizae Radix and Carthami Flos) pair with different preparations and proportions when decocting together by using HPLC-PDA and fuzzy chemical identification strategy with poly-proportion design. Methods: A simple project was initially developed for the rapid identification and classification of four types of components in Danshen-Honghua based on the establishment of relevant component data base, recognition of the reference compound peaks, selection of the characteristics of ultraviolet spectrum, and formation of group networks. In this study, the accurate structures of the chemical components did not need to be determined, and only the constituents attributed to different groups were further considered for quantitative analysis. Results: A total of 47, 57, and 43 constituents from different preparations of Danshen-Honghua were classified into four kinds of chemical groups, and they were quantitatively analyzed furtherly according to semi-symmetric proportion design. The results showed that the preparation of methanol-water could significantly promote the dissolution of most salvianolic acids, chalcones, and flavonoids, while the contents of tanshinones in methanol were higher than others. The relative dissolution rate range of total optimization for the four types of components were from 5:1 to 5:2, and the highest proportion of the total relative dissolution were all 5:2. Conclusion: The most reasonable range may appear 5:1-5:2 for water preparation and be consistent with clinical ratio, which provides the reference for clinical application of Danshen-Honghua pair, and for modern research approach of herb pairs.
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Objective: To evaluate antioxidant activities of seven medicinal plant species and their fractions, and to identify their phenolic compounds. Methods: Two extractions were processed and further fractionated by column chromatography to evaluate the concentration that inhibit 50% of 2,2'-azinobis (3-ethylbenzothiazoline-6-suslfonic acid, 1,1-diphenyl-2-picryl-hydrazyl radicals, and their ferric reducing antioxidant power. The identification of the fractions of phenolic compounds was done by ultra performance liquid chromatography. Results: The aqueous-acetone extracts of Feretia apodanthera and Ozoroa insignis exhibited the highest antioxidant potentials comparable to those of the standard quercetin. Their subsequently silica gel column fractionation showed three most active fractions from which the major constituents quercetin, myricetin, kampferol, rutin and isoquercetin were identified. Conclusions: These plant species have potent antioxidant profiles and polyphenol compounds that may help to manage with radical related disease and aging.
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OBJECTIVE@#To evaluate antioxidant activities of seven medicinal plant species and their fractions, and to identify their phenolic compounds.@*METHODS@#Two extractions were processed and further fractionated by column chromatography to evaluate the concentration that inhibit 50% of 2,2'-azinobis (3-ethylbenzothiazoline-6-suslfonic acid, 1,1-diphenyl-2-picryl-hydrazyl radicals, and their ferric reducing antioxidant power. The identification of the fractions of phenolic compounds was done by ultra performance liquid chromatography.@*RESULTS@#The aqueous-acetone extracts of Feretia apodanthera and Ozoroa insignis exhibited the highest antioxidant potentials comparable to those of the standard quercetin. Their subsequently silica gel column fractionation showed three most active fractions from which the major constituents quercetin, myricetin, kampferol, rutin and isoquercetin were identified.@*CONCLUSIONS@#These plant species have potent antioxidant profiles and polyphenol compounds that may help to manage with radical related disease and aging.
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Objective To provide scientific evidence for the identification and development of fructus psoraleae through its pharmacognostical study.Methods To identify fructus psoraleae by paraffin section and powder method,meanwhile,morphological identification and physical and chemical identification were also adopted.Results The morphological and microscopic characteristics of transverse section and powder of fructus psoraleae were described; fluorescent speckles of the same color were found in the same place of test samples and psoralen and isopsoralen reference by thin layer chromatography; results of water and ash determination all complied with Chinese pharmacopoeia specification,water and 70％ ethanol extractives were 21.93～39.68％,22.03～31.77％,respectively.Conclusion The established method was simple and practical with reliable results,and suitable for the identification of fructus psoraleae.