Clinical analysis of 11 patients with phytophotodermatitis caused by ingesting Chenopodium album / 中华皮肤科杂志

Objective@#To investigate clinical characteristics and treatment of phytophotodermatitis due to ingesting Chenopodium album.@*Methods@#This study included 11 patients with phytophotodermatitis caused by ingesting Chenopodium album collected from Department of Dermatology, Affiliated Hospital of Jining Medical University from 2013 to 2017. The patients′ general information, clinical manifestations, laboratory test results, treatment and prognosis were retrospectively analyzed.@*Results@#All the 11 patients were female, and their age ranged from 45 to 62 years. They all had a history of ingesting Chenopodium album and exposing to sunlight within 1 - 2 days prior to the disease onset. Clinical manifestations included symmetrically distributed, painful and pruritic, nonpitting, swelling erythema on the face and back of both hands and at sunexposed sites of forearms, with a tense and bright surface. Increased white blood cell counts were observed in 6 patients, and increased eosinophil counts in 1. All of the 11 patients were treated with systemic methylprednisolone, loratadine, ebastine, spironolactone, furosemide and omeprazole as well as topical agents, 2 also received human immunoglobulin treatment, and 3 were also treated with oral ibuprofen and codeine for painful lesions. Ten patients received obvious improvement and were discharged after 7 - 10 days of treatment, and no pigmentation or scars were observed after 1-year follow-up. Skin necrosis occurred on the back of both hands in 1 patient after 7-day treatment, and scars remained in the patient after follow-up of half a year.@*Conclusions@#Chenopodium album-induced phytophotodermatitis commonly manifests as swelling erythema on the exposed body sites. After confirmed diagnosis, Chenopodium album ingestation and sunlight exposure should be avoided, and timely antianaphylactic treatment should be considered to effectively control the disease.

Clinical analysis of 11 patients with phytophotodermatitis caused by ingesting Chenopodium album / 中华皮肤科杂志

Objective To investigate clinical characteristics and treatment of phytophotodermatitis due to ingesting Chenopodium album.Methods This study included 11 patients with phytophotodermatitis caused by ingesting Chenopodium album collected from Department of Dermatology,Affiliated Hospital of Jining Medical University from 2013 to 2017.The patients' general information,clinical manifestations,laboratory test results,treatment and prognosis were retrospectively analyzed.Results All the 11 patients were female,and their age ranged from 45 to 62 years.They all had a history of ingesting Chenopodium album and exposing to sunlight within 1-2 days prior to the disease onset.Clinical manifestations included symmetrically distributed,painful and pruritic,nonpitting,swelling erythema on the face and back of both hands and at sunexposed sites of forearms,with a tense and bright surface.Increased white blood cell counts were observed in 6 patients,and increased eosinophil counts in 1.All of the 11 patients were treated with systemic methylprednisolone,loratadine,ebastine,spironolactone,furosemide and omeprazole as well as topical agents,2 also received human immunoglobulin treatment,and 3 were also treated with oral ibuprofen and codeine for painful lesions.Ten patients received obvious improvement and were discharged after 7-10 days of treatment,and no pigmentation or scars were observed after 1-year follow-up.Skin necrosis occurred on the back of both hands in 1 patient after 7-day treatment,and scars remained in the patient after follow-up of half a year.Conclusions Chenopodium album-induced phytophotodermatitis commonly manifests as swelling erythema on the exposed body sites.After confirmed diagnosis,Chenopodium album ingestation and sunlight exposure should be avoided,and timely antianaphylactic treatment should be considered to effectively control the disease.

Ameliorating potency of Chenopodium album Linn. and vitamin C against mercuric chloride-induced oxidative stress in testes of Sprague Dawley rats

BACKGROUND@#Mercury has been documented as an industrial risk that posed a serious danger to human health. Mercury exposure results in oxidative stress that may lead to the pathogenesis of male reproductive dysfunction. The present study investigated the ameliorating potential of Chenopodium album L. and vitamin C against mercuric chloride-induced oxidative deterioration of reproductive functions in adult male rats.@*METHODS@#Group 1 (control) received saline. Group 2 received Mercury (0.15 mg/kg b.w, i.p) dissolved in distilled water. Groups 3 and 4 were given oral gavage of vitamin C (200 mg/kg b.w) and the ethanolic extract of C. album (200 mg/kg b.w) respectively, along with Mercury (0.15 mg/kg b.w, i.p). Group 5 was treated only with C. album (200 mg/kg b.w). After 30 days of the treatment, the rats were dissected and their testicular tissue and the cauda epididymis were used for biochemical analysis while blood plasma was used for protein determination.@*RESULTS@#The applied dose-treatment of Mercury-induced oxidative stress in the testis and cauda epididymis tissues of the rats was apparent by a noteworthy decrease in total protein, CAT, SOD, POD, and GST values while there was increase in ROS and TBARS levels. Furthermore, Mercury decreases daily sperm production and enhanced sperm DNA damage as noticeable by an increase in the head and tail length of comets and decrease in intact DNA. There was no significant effect on the body weight and the weight of the reproductive tissues. Treatment with C. album significantly ameliorated the total protein, ROS, and TBARS content. Similarly, the level of CAT, SOD, POD, and GST was significantly improved and the daily sperm production was significantly increased. Furthermore, C. album administration significantly protected Mercury-induced sperm DNA damage. The results of the extract treatment group were compared with those of vitamin C in detoxifying the oxidative stress and restoring the sperm parameters.@*CONCLUSION@#C. album showed protection against Mercury-induced oxidative stress by ameliorating antioxidant enzyme activity, daily sperm production, and DNA damage in rat testes. This suggests that C. album could be beneficial against toxicity induced by an environmental toxicant.

Efecto de la temperatura de conservación sobre la expresión de proteinas en granos de polen de chenopodium album l / Effect of conservation temperature on the expression of proteins in pollen grains of chenopodium album l

Los componentes activos del polen a partir del cual se obtienen los extractos alergénicos pueden variar considerablemente deacuerdo al momento, el lugar dónde se recolecta y el períodocomprendido entre la recolección y su utilización.El presente trabajo pretende evaluar si la temperatura de conservacióndel grano de polen influye en la expresión de proteínasy en la antigenicidad de las mismas, al momento de prepararun extracto alergénico. La especie elegida para estudio fue Chenopodiumalbum L. ya que es de gran interés alergológico en laciudad de Bahía Blanca. Los granos de polen se conservaron a temperatura ambiente, 4 °Cy -18 °C por el término de dos meses. El contenido proteico de losextractos se determinó por el Método de Bradford. La expresiónproteica y la antigenicidad se estudiaron mediante electroforesisvertical Tricina-PAGE-SDS 12, 5 % e Inmunoblot respectivamente.Los resultados obtenidos demuestran que la concentración proteicatotal fue menor para los extractos obtenidos del polen conservadoa temperatura ambiente que para las otras dos condiciones.La expresión de proteínas varía cuantitativamente en todoslos extractos y si bien la expresión cualitativa prácticamente seconserva, aparece para el polen conservado a temperatura ambiente,una banda de PM menor a 12 kDa. Esta banda podríaser consecuencia de la degradación proteica que experimenta elpolen a esa temperatura de almacenamiento. En cuanto a la antigenicidadde los extractos no hay diferencias cualitativas aunquepueden apreciarse diferencias cuantitativas significativas.Concluimos que la conservación del polen a 4°C o a -18°C seríanlas más adecuadas, ya que permiten obtener una mayor concentraciónproteica partiendo de la misma masa de polen.

The active components from which pollen allergen extracts are obtainedcan change considerably according to the time or the placewhere collect and the time period between harvesting and use.This work aims to assess if the storage temperature of the pollengrain influences protein expression and its antigenicity when preparingan allergen extract. The species chosen for our study wasChenopodium album L, since it is of great allergologic interest inthe city of Bahía Blanca.Pollen grains were stored at room temperature 4ºC and -18ºC ,for a period of two months. The protein content of the extractswas determined by the Bradford method. Protein expressionand antigenicity were studied by vertical electrophoresis TricineSDS-PAGE 12, 5% and Immunoblot. The obtained results show that the total protein concentrationwas lower in the extracts of pollen stored at room temperaturethan in those under two conditions. Protein expression differsquantitatively in all extracts and even if the qualitative expressionis kept practically the same, in the Tricine SDS-PAGE geland the pollen stored at room temperature there appear a bandof MW inferior to 12 kDa. This band could result from the proteindegradation experienced by pollen stored at that temperature.As regards extract antigenicity, there are no qualitative differenceseven though there are significant quantitative differences.We conclude that pollen preservation at 4ºC or -18ºC would bethe most appropriate since it allows greater protein concentrationfor the same mass of pollen.

Evaluation of antibacterial activities of chenopodium album L.

The present study was aimed to evaluate the antibacterial activities of Chenopodium album L. against five human pathogenic bacteria. Cheopodium album L. is belonging to family Chenopodiaceae and commonly known as Bathua. The collected leaves were washed, dried and powdered. Aqueous and methanol extracts were prepared and observed their antibacterial activity against human pathogenic bacteria Viz. Escherichia coli, Salmonella typhimurium, Staphylococcus aureus, Proteus vulgaris and Pseudomonas aueruginosa The significant results were obtained by aqueous as well as methanol leaf extract on tested pathogens using paper disc diffusion method. The aqueous extract revealed strongest antibacterial activity on Staphylococcus aureus and methanol leaf extract showed strongest antibacterial activity on Pseudomonas aeruginosa.

Chenoalbuside: an antioxidant phenolic glycoside from the seeds of Chenopodium album L. (Chenopodiaceae)

In addition to three known phytoecdysteroids, a new phenolic glycoside (named, chenoalbuside) was isolated from the methanol extract of the seeds of Chenopodium album. While the structures of all phytoecdysteroids were elucidated by direct comparison of their spectroscopic data with published data, the structure of chenoalbuside was determined unequivocally by a combination of UV, MS and 1D and 2D NMR spectroscopic analyses. The antioxidant potential of the new compound was assessed by the DPPH assay, and the RC50 value was found to be 1.4 x 10-4 mg/mL.