RESUMEN
OBJECTIVE:To investigate the antiviral action of emodin in combination with other Chinese active medicine components on Coxsackie virus B3(CVB3) and explore the possible antiviral mechanism.METHODS:Hep-2 cells infected by CVB3 were cultured with different concentration and proportion of the emodin and other Chinese medicine components for 72 hours and their inhibitory effects on CVB3 replication were evaluated with cell survival rates by MTT assay.RESULTS:In the Hep-2 cell system,all the combinations between emodin and other medicine components could inhibit cytopathic effect(CPE) of CVB3-infected cells,increase cells' survival rates and decrease the cytotoxicity.The drug combination which showed the best effect of killing viruses directly was emodin:curcumine:banlangen(2:1:2),and the one showing the highest efficacy in inhibiting the replication of CVB3 in cells was emodin:curcumine:banlangen(2:0:1).CONCLUSION:The toxicity of emodin was reduced and its anti-CVB3 activity was enhanced by its combined use with other Chinese active medicine components.