Limb immobilization alters functional electrophysiological parameters of sciatic nerve

Immobilization, used in clinical practice to treat traumatologic problems, causes changes in muscle, but it is not known whether changes also occur in nerves. We investigated the effects of immobilization on excitability and compound action potential (CAP) and the ultrastructure of the rat sciatic nerve. Fourteen days after immobilization of the right leg of adult male Wistar rats (n=34), animals were killed and the right sciatic nerve was dissected and mounted in a moist chamber. Nerves were stimulated at a baseline frequency of 0.2 Hz and tested for 2 min at 20, 50, and 100 Hz. Immobilization altered nerve excitability. Rheobase and chronaxy changed from 3.13±0.05 V and 52.31±1.95 µs (control group, n=13) to 2.84±0.06 V and 59.71±2.79 µs (immobilized group, n=15), respectively. Immobilization altered the amplitude of CAP waves and decreased the conduction velocity of the first CAP wave (from 93.63±7.49 to 79.14±5.59 m/s) but not of the second wave. Transmission electron microscopy showed fragmentation of the myelin sheath of the sciatic nerve of immobilized limbs and degeneration of the axon. In conclusion, we demonstrated that long-lasting leg immobilization can induce alterations in nerve function.

Potencial de ação: do estímulo à adaptação neural / Action potential: from excitation to neural adaptation

INTRODUÇÃO: O potencial de ação (PA) origina-se graças a uma perturbação do estado de repouso da membrana celular, com consequente fluxo de íons, por meio da membrana e alteração da concentração iônica nos meios intra e extracelular. OBJETIVOS: Sintetizar o conhecimento científico acumulado até o presente sobre o potencial de ação neural e o seu processo de adaptação sob aplicação de um estímulo constante. MATERIAIS E MÉTODOS: Busca realizada nas bases Springer, ScienceDirect, PubMed, IEEE Xplore, Google Acadêmico, Portal de Periódicos da Capes, além de livros referentes ao assunto. O idioma de preferência selecionado foi o inglês, com as keywords: action potential; adaptation; accommodation; rheobase; chronaxy; nerve impulse. Efetuou-se a procura de artigos com uma janela de tempo de 1931 a 2010 e livros de 1791 a 2007. RESULTADOS: Dos trabalhos selecionados, foram extraídas informações a respeito dos seguintes tópicos: potencial de ação e suas fases; condução nervosa; reobase; cronaxia; acomodação; e adaptação neuronal. CONCLUSÃO: Um estímulo que crie PA, se aplicado de maneira constante, pode reduzir a frequência de despolarizações em função do tempo e, consequentemente, adaptar a célula. O tempo que a célula demora, na ausência de estímulos, para recuperar sua frequência original é definido como desadaptação.

INTRODUCTION: The action potential (AP) arises due to a disturbance of the resting state of the cell membrane with consequent flow of ions across the membrane and ion concentration changes in intra and extra cellular space. OBJECTIVES: This article aims to summarize the scientific knowledge accumulated to date on the action potential and neural adaptation in the process of applying a constant stimulus. MATERIALS AND METHODS: This is a literature review on the bases Springer, ScienceDirect, PubMed, IEEE Xplore, Google Scholar, Capes Periodicals Portal as well as books on the topic. The selected preferred language was English with the keywords: action potential; adaptation, accommodation; rheobase; chronaxy; nerve impulse. We conducted a search of articles with a wide time window from 1931 to 2010 and books from 1791 to 2007. RESULTS: In the selected studies was extracted information about the following topics: action potential and its stages; nerve conduction; rheobase; chronaxie, accommodation, and adaptation. CONCLUSION: A stimulus that creates AP, if applied consistently, can reduce the frequency of depolarization as a function of time and, consequently, to adapt the cell. The time it takes the cell in the absence of stimuli, to recover its original frequency, is defined as a disadaptation.