RESUMEN
BACKGROUND:Studies have shown that chronic apical periodontitis is one of the common inflammatory bone destruction diseases.Icariin can promote osteogenic differentiation,inhibit bone resorption,and may play a protective role in bone destruction caused by chronic apical periodontitis. OBJECTIVE:To investigate the effect of icariin on the proliferation and differentiation of MC3T3-E1 cells in the inflammatory environment stimulated by lipopolysaccharides. METHODS:Lipopolysaccharides were used to stimulate MC3T3-E1 cells to establish an inflammatory environment in vitro,and cell counting kit-8 was used to detect the best concentration and optimal action time of lipopolysaccharides.Cell counting kit-8 was used to detect the optimal concentration of icariin under the stimulation of lipopolysaccharides at a concentration of 1 μg/mL.Alkaline phosphatase detection,Real-time PCR and western blot assay were used to detect the effect of icariin on osteogenic differentiation of MC3T3-E1 cells in the inflammatory environment.Real-time PCR and western blot were used to detect the effects of icariin on the expression of interleukin-1β and interleukin-6 in MC3T3-E1 cells in the lipopolysaccharide-stimulated inflammatory environment. RESULTS AND CONCLUSION:Cell counting kit-8 results showed that the optimal concentration of icariin was 0.1 μg/mL.In the inflammatory environment,icariin enhanced the expression of alkaline phosphatase and promoted osteoblast differentiation.Compared with the lipopolysaccharide group,the expression of osteogenesis-related factors alkaline phosphatase and Runx2 was increased in the lipopolysaccharide+icariin group.Compared with the lipopolysaccharide group,the expression levels of inflammation-related factors interleukin-1β and interleukin-6 decreased in the lipopolysaccharide+icariin group.To conclude,lipopolysaccharides weaken the osteogenic ability of MC3T3-E1 cells and aggravate the inflammatory response,but icariin has a protective effect on them.
RESUMEN
Objecive:To observe the clinical effects of one-off root canal therapy using iRoot SP in the treatment of chronic apical periodontitis with sinus in anterior teeth.Methods:240 anterior teeth of chronic apical periodontitis witn sinus were randomly divided into 2 groups(n=120).One-off root canal filling were performed using iRoot SP(group A) and AH-plus(group B) respectively after Nd:YAG laser disinfection.Clinical effects were evaluated 48 h,10 days and 1 year after treatment.Results:In group A and B,the 48 h postoperative pain reaction rate was 7.14% and 15.0%(P0.05) respectively.Conclusion:iRoot SP and AH-plus show reliable effect in the treatment of chronic apical periodontitis with sinus in anterior teeth with one-off root canal therapy.iRoot SP may result in sligher postoperative reaction and shorter healing time.
RESUMEN
Objective:To investigate the effect of Vitapex paste in the root canal for deciduous teeth with chronic apical periodontitis.Methods:200 children with chronic apical periodontitis were selected and divided into two groups.The observation group (108 cases)was given Vitapex paste.The control group (92 cases) was given Zinc oxide iodoform paste.The effect of Vitapex paste in root canal for deciduous teeth with chronic apical periodontitis was evaluated by filling quality after 1 month'treatment,the VAS scores before and after treatment,long-term curative effect were compared between two groups.Results:After 1 month'treatment,the suitable filling rate of observation group was higher than that of the control group(P<0.05).There was no statistical significance in the VAS scores between two groups (P>0.05).After 1 week'and 1 month'treatment,the pain feeling were alleviated in two groups and significantly lighter in the observation group(P<0.05).After 6 months' follow-up,the success rate was 96.3% in the observation group and 87.0% in the control group,the success rate of observation group was higher than that of the control group (P<0.05).Conclusion:The filling process of Vitapex paste was simple and easy,which had a good therapeutic effect on chronic apical periodontitis of deciduous teeth and could relieve the toothache of patients.
RESUMEN
O presente relato de caso tem como objetivo descrever e discutir o tratamento de um dente incisivo central superior direito tratado endodonticamente, com presença de reabsorção radicular apical e radiolucencia periapical. Ao exame clínico a paciente se encontrava com fístula na região apical da mucosa vestibular do referido dente. Ao exame por imagens a radiografia periapical e tomografia computadorizada conebeam, revelaram a presença de área radiolúcida no terço apical, dentro do canal radicular, inacessível aos procedimentos do preparo químico-mecânico tornado o retratamento endodôntico incerto. Dessa forma, a cirurgia perirradicular foi a terapêutica instituída, com a realização da apicectomia, retropreparo e retrobturação do canal radicular, com uso de insertos ultrassônicos apropriados e do Agregado Trióxido Mineral (MTA), respectivamente. Após dois anos de proservação pôde-se observar o controle da infecção endodôntica. O tratamento foi considerado um sucesso, visto que a paciente se encontrava assintomática, com ausência de fístula e com regressão completa da radiolucência periapical e neoformação ãssea. Pode-se concluir que a cirurgia perirradicular apresentou-se como um tratamento eficaz para a resolução do referido caso clínico, possibilitando um grau de desinfecção compatível com a cura perirradicular para o paciente... (AU)
This case report aims to describe and discuss the treatment of a right maxillary central incisor tooth, endodontically treated, with presence of an internal root resorption and periradicular radiolucency. On clinical examination the patient presented a sinus tract at the apical buccal mucosa of this tooth. The periapical radiograph and cone beam computed tomography detected the presence of radiolucent area inside root canal (apical third). This area showed to be inaccessible to adequate chemical-mechanical preparation, so, the endodontic retreatment would give an uncertain prognosis. Thus, the periradicular surgery was the indicated therapy for this case, including apicectomy, retropreparation and root canal retrofilling, with the use of appropriated ultrasonic inserts and Mineral trioxide aggregate (MTA), respectively. After two years of follow-up, the endodontic infection control was observed. The treatment was considered a success, as the patient was asymptomatic, with no sinus tract and with complete regression of periapical radiolucency and bone formation. It can be concluded that the periradicular surgery was an effective treatment for the patient, allowing a degree of disinfection compatible with periradicular cure... (AU)
Asunto(s)
Humanos , Femenino , Adolescente , Apicectomía , Pérdida de Hueso Alveolar , Enfermedades PeriapicalesRESUMEN
Introducción: determinar la prevalencia de periodontitis apical crónica en dientes tratados endodónticamente en lacomunidad académica de la Universidad Cooperativa de Colombia, Pasto. Métodos: se diseñó un estudio transversal, en donde se incluyeron 37 personas y 49 dientes tratados endodónticamente. Estos fueron evaluados por dos examinadores para determinar la prevalencia de periodontitis apical crónica y variables relacionadas como edad, sexo, dientes, calidad del tratamiento de conducto,caries dental, tipo y calidad de la restauración. El índice de Kappa de Cohen interexaminador fue tomado. Resultados: periodontitis apical crónica (PAC) fue evidente en 79,6% de todos los dientes con tratamiento de conducto. De estos 56,4% pertenecieron ahombres y 43,6% a mujeres. Los dientes premolares obturados tuvieron prevalencia de PAC mayor a molares. De acuerdo con el sellado de las obturaciones, solamente el 24,3% con sellado adecuado no tenía periodontitis apical. El 12,2% de los dientesobturados fueron considerados adecuados técnicamente. Dientes restaurados con resina tuvieron PAC más severa. Conclusiones: este estudio determinó que en esta población hay alta prevalencia de periodontitis apical crónica. Variables como género, tipo dediente, calidad del tratamiento de conducto, caries dental y calidad de la restauración influyen sobre la presencia de PAC.
Introduction: to determine the prevalence of chronic apical periodontitis in endodontically treated teeth in the academic community at Universidad Cooperativa de Colombia, Pasto. Methods: a cross-sectional study was designed where a sample of 37 individuals and 49 endodontically treated teeth were included. They were screened by two examiners to determinethe prevalence of chronic apical periodontitis and related variables such as age, gender, teeth, quality of endodontic filling, dental caries, and type and quality of restoration. Inter-examiner Cohens Kappa index was done. Results: chronic apical periodontitis (CAP) was evident in 79.6% of all endodontic treated teeth. Of teeth with CAP, 56.4% belonged to men and 43.6% to women. Premolars had a greater prevalence of chronic apical periodontitis than molars. According to endodontic fillings seal, only 24.3% with adequate seal did not have chronic apical periodontitis. The 12.2% of the endodontically treated teeth were considered technically adequate. Teeth restored with composite had severe CAP. Conclusions: this study determined that in this population there is a high prevalence of chronic apical periodontitis. Variables such as gender, type of tooth, root filling quality, dental caries and restoration quality influence the CAP presence.
Asunto(s)
Humanos , Estudios Transversales , Periodontitis , Endodoncia , PrevalenciaRESUMEN
OBJECTIVE: The aims of this study were to investigate the presence of Enterococcus faecalis in primary endodontic infections and failed endodontic treatments using real-time PCR and to determine the statistical importance of the presence of E. faecalis in a Turkish population with endodontic infections. MATERIAL AND METHODS: E. faecalis was investigated from 79 microbial samples collected from patients who were treated at the Endodontic Clinic of the Dental School of Atatürk University (Erzurum, Turkey). Microbial samples were taken from 43 patients (Group 1) with failed endodontic treatments and 36 patients (Group 2) with chronic apical periodontitis (primary endodontic infections). DNA was extracted from the samples by using a QIAamp® DNA mini-kit and analyzed with real-time PCR SYBR Green. RESULTS: E. faecalis was detected in 41 out of 79 patients, suggesting that it exists in not less than 61 percent of all endodontic infections when the proportion test (z= -1.645, Asunto(s)
Humanos
, Enfermedades de la Pulpa Dental/microbiología
, Enterococcus faecalis/aislamiento & purificación
, Infecciones por Bacterias Grampositivas/microbiología
, Reacción en Cadena de la Polimerasa/métodos
, Tratamiento del Conducto Radicular
, Técnicas Bacteriológicas
, Enfermedad Crónica
, ADN Bacteriano/análisis
, Cavidad Pulpar/microbiología
, Enfermedades de la Pulpa Dental/terapia
, Infecciones por Bacterias Grampositivas/terapia
, Periodontitis Periapical/microbiología
, Periodontitis Periapical/terapia
, Pulpitis/microbiología
, Pulpitis/terapia
, Preparación del Conducto Radicular/métodos
, Insuficiencia del Tratamiento
, Turquía
RESUMEN
Esta pesquisa foi realizada em 32 pacientes acima de 60 anos de idade atendidos nas clínicas de Semiologia, Prótese Dentárias e Clínica Integrada da graduação das Faculdades de Odontologia da UPE, UFPE e FOR, com o objetivo de avaliar a prevalência de Periodontite Apical Crônica (P.A.C.)nestes idosos através de axames radiográficos correlacionando esses achados com fatores como: idade, gênero, escolaridade, dente mais acometido, número de dentes presentes na cavidade oral e estado geral do paciente. A presença da P.A.C. foi confirmada em apenas cinco (15,6%) idosos, portanto a prevalência da lesão foi considerada baixa, mesmo levando em consideração a pequena amostra; a presença da mesma foi registrada em 8 dentes, destes pacientes destaca-se que: quatro era do sexo feminino, com idades variando entre 61 a 69 anos, sendo que três tinham 67 anos; todos tinham hipertensão arterial; em relação à escolaridade registrou-se que um paciente era analfabeto, um tinha primeiro grau incompleto e três tinham 1º grau completo.
This study done on 32 pacients 60 years odl and over, who received care at the clinics of semiology, prosthodontics, and integrated clinic of graduation of the dentistry scholls of UPE, ufpe, and FOR, with the purpose of evaluating the prevalence of Chronic Apical Periodontitis on these people of age through radiographic examinations, correlating these findings with factors such as: age, gender, level of education, more tooth, number of teeth present in the oral cavity, and overall state of the pacient. The presence of Chronic Apical Periodontitis was confirmed on only 5 (15.6%) pacients, wich was a low prevalence of the lesion; the presence of the same was registered in 8 teeth, of these pacients it is notable that: 4 were females, with age varying from 61 to 69 years, with 3 being 67 years old; each one had arterial hypertension; in relation to level of education, it was noted that 1 pacient was illiterate.
Asunto(s)
Anciano , Cuidado Dental para Ancianos , Odontología Geriátrica , Periodontitis PeriapicalRESUMEN
The treatment of a large dentine caries, which seemed to be a small pit-and-fissure carious lesion on the occlusal surface of the permanent mandibular right first molar in a 6-year-old boy, is presented. The treatment included the filling of the root canals and the sealing of the cavity with composite resin. Underlying periodontal tissue healing could be radiographically observed at the 14-month follow-up. It is possible that a better visual examination, with careful cleaning and drying of teeth, may improve occlusal caries detection. Dentists should examine bitewing radiographies carefully for occlusal demineralization. As radiographies are probably an effective method of lesions caries diagnosis, they should be recommended at appropriate ages to aid early detection of these lesions.
O tratamento de uma cárie grande do dentine, que pareça ser um lesion carious do poço-e-pit-and-fissure pequeno na superfície occlusal do primeiro molar da direita mandibular permanente em um menino de 6-year-old, é apresentado. O tratamento inclui o enchimento dos canais da raiz e do sealing da cavidade com resina composta. O tecido peridental subjacente que healing podia radiographically ser observado na continuação de 14 mês. É possível que uma examinação visual melhor, com limpeza cuidadosa e secagem dos dentes, pode melhorar a deteção occlusal da cárie. Os dentistas devem examinar radiographies do bitewing com cuidado para o demineralization occlusal. Porque as radiografias são provavelmente um método eficaz do diagnóstico da cárie dos lesions, devem ser recomendados em idades apropriadas ajudar à deteção adiantada desta lesão.
Asunto(s)
Niño , Diagnóstico , Periodontitis Periapical , Radiografía DentalRESUMEN
This syndrome, also known as encephalotrigeminal angiomatosis, is a condition which includes vascular anomalies and intra-oral involvement. A patient came to the Dentistry School's endodontic clinic with complaints of localized pain on the lateral superior left teeth. She had a previous diagnostic of Sturge-Weber syndrome confirmed by the presence of bilateral facial nevus flammeus, examinations of angiography, radiological evidence of calcific densities, and ipsilaterally intra-oral vascular hyperplasia in the lip, cheek and tongue. Dental management included plaque control instructions, root planing and dental restoration. Particular attention is given to oral clinical changes, there were no complications and the tissue healed normally.
Esta síndrome, também conhecida como angiomatose encéfalo trigeminal, é uma condição que inclui anormalidades vasculares e envolvimento intra-oral. Um paciente procurou à Clínica de Endodontia da Faculdade de Odontologia de Pernambuco com queixa de dor localizada no dente incisivo lateral superior. A paciente já possuia diagnóstico prévio da síndrome de Sturge-Weber confirmado pela presença de nevos flâmeos localizados bilateralmente na fse, angiografias, evidencias radiológicas de calcificações, e hiperplasia vascular ipsilateral do lábio, língua e região jugal. O tratamento odontológico incluiu instruções para controle de placa, tratamento endodôntico e restaurações dentária. Acompanhamento pós-operatório foi realizado cuidadosamente e a cura ocorreu sem complicações.