Chemical constituents from roots of Lindera aggregate / 中草药

Objective: To study the chemical constituents from the roots of Lindera aggregata. Methods: The compounds were isolated and purified by various column chromatographies. Their structures were elucidated by means of spectral analyses (MS, 1D-NMR, and 2D-NMR). Results: Fourteen compounds were isolated from 95% ethanol extract of L. aggregate. Compound 1 was isolated as a new one and named as bi-linderachalcone (1), and the other compounds were identified as methyllinderone (2), linderone (3), pashanone (4), pinostrobin (5), methyllucidone (6), pinocembrin (7), 5,7-dihydroxy-6,8-dimethoxyflavone (8), lucidone (9), ethyllucidone (10), cinnamic acid (11), pinostrobinchalcone (12), cirsimaritin (13), and β-sitosterol (14), respectively. Conclusion: Compound 1 is isolated as a new compound and compound 13 is firstly obtained from the plants of Lindera Thunb..

Study on chemical constituents of Artemisia integrifolia / 中草药

Objective: To study the chemical constituents from the aerial parts of Artemisia integrifolia. Methods: The chemical constituents were separated and purified by column chromatographies and HPLC. Their structures were determined on the basis of spectroscopic analyses (1H-NMR, 13C-NMR, 2D-NMR, and MS). Results: Fifteen compounds were isolated from the methanol extract in the aerial parts of A. integrifolia with the structures identified as α-curcumene (1), β-sitosterol-3-O-β-D-glucoside (2), zingiberone (3), 3-(2-hydroxyphenyl) propanoic acid methyl ester (4), (E)-o-hydroxycinnamic acid (5), eupatorin (6), cirsimaritin (7), artemetine (8), loliolide (9), luteolin-7-O-β-D-glucopyranoside (10), (+)-pinoresinol (11), α-spinasterol (12), reynosin (13), 3α-hydroxy-1(10),4,11(13)-diager-12,6α-olide (14), and scopoletin (15). Conclusion: Compounds 1 and 3 are isolated from the plants of Artemisia Linn for the first time and compounds 4-9, 11, 13, and 14 are isolated from this plant for the first time.

Antigiardial activity of flavonoids from leaves of Aphelandra scabra

AbstractAphelandra scabra (Vahl) Sm., Acanthaceae, is a shrub widely used by some Mayan communities as carminative, antidote, and remedy for some infections. Bio-guided isolation of the methanol extract of leaves led us to the purification of the anti-giardial metabolites cirsimaritin and sorbifolin, along with the inactive metabolites cirsimarin, sorbifolin-6-O-β-glucopyranoside, and squalene. Cirsimaritin displayed high activity in the anti-giardial bioassay with an IC50 = 3.8 μM, being considered as outstanding when compared to previous reported metabolites, while sorbifolin showed a low activity with an IC50 = 75.6 μM. Additionally, both compounds proved not to be cytotoxic in an in vitro bioassay against HEK-293, a normal cell line. This is the first investigation on anti-giardial properties of A. scabra and its phytochemistry as well, thus the isolated compounds are considered as new for the plant genus and for the species.

Chemical constituents from stems of Pleioblastus amarus / 中草药

Objective: To study the chemical constituents from the stems of Pleioblastus amarus. Methods: The compounds were isolated and purified by means of chromatographic techniques and their structures were identified on the basis of spectral data. Results: Fifteen known phenolic compounds were isolated in the methanol extract from the dry stems of P. amarus and thire structures were identified as tricin (1), apigenin (2), 5, 7, 2′, 4′, 6′-pentamethoxyflavone (3), 5, 7-dihydroxy-3′, 4′, 5′-trimethoxyflavone (4), acacetin (5), luteolin (6), cirsimaritin (7), p-hydroxybenzaldehyde (8), 2-hydroxybenzoic acid (9), trans-p-hydroxycoumaric acid (10), 2, 4-dihydroxybenzaldehyde (11), gallic acid (12), methyl gallate (13), questin (14), and isorhodoptilometrin (15). Conclusion: Compounds 2-7 and 10-13 are first obtained from this plant; Compounds 14 and 15 are first isolated from the plants of Pleioblastus Nakai.