RESUMEN
Objective To study the mechanism of attenuated Strychni Semen (SS) and Glycyrrhizae Radix et Rhizoma (GRR) compatibility with the phase angle as the starting point. Methods The phase separation of SS single decoction and compatibility decoction was carried out by natural sedimentation, dialysis and high-speed centrifugation. UPLC-MS assay was used to study the differences in the distribution of brucine and strychnine in four different phases, and the change of the amount of toxic substances was analyzed. Results The compatibility of the decoction can be divided into four groups: true solution group, colloidal solution group, suspension group, and sediment group. Brucine and strychnine were well separated under the selected chromatographic conditions. The linear relationship was good in the range of 0.020-2.008 and 0.022-2.236 μg (r = 0.999 8), and the recovery was 96.84% and 97.62%, which indicated that the method was good. The distribution of brucine and strychnine in the different phase of the single decoction showed the same variation regularity, colloid group > solution group > sediment group > suspended group; Compatibility decoction of different phase of brucine and strychnine also showed the same distribution, sediment group > colloidal group > suspension group > solution group. Conclusion The contents of toxic substances in SS were decreased after compatibility with GRR, especially for the strongest toxicity of strychnine, which mainly concentrated in the sediment components. Studies on the changes of toxic substances in phase states indicated that the sustained release effect of the sediments components after compatibility with GRR is the key factor of reducing toxicity and improving efficiency.
RESUMEN
Objective: To investigate the effect of colloidal solution infusion on cerebral blood flow in patients with cerebral vasospasm after aneurysmal subarachnoid hemorrhage. Methods: Thirty patients diagnosed as aneurysmal subarachnoid hemorrhage with digital subtraction angiography (DSA) were divided into a vasospasm group (n = 12) and a control group (n = 18) according to whether they had cerebral vasospasm or not. After evaluating the regional cerebral blood flow (rCBF) of the bilateral anterior cerebral artery, middle cerebral artery and posterior cerebral artery with Xe-CT, the colloidal solution (succinylated gelatin, 4 mL/kg) was injected intravenously in the patients of the vasospasm group within 30 minutes. Xe-CT was performed again, and the changes of rCBF before and after the colloidal solution expansion were compared. The changes of blood pressure, heart rate, and central venous pressure before and after infusion were documented. Results: Circled digit oneThe rCBF of bilateral anterior cerebral artery, middle cerebral artery and posterior cerebral artery was more than 30 mL·100 g-1·min -1 in the control group. The rCBF was lower than 30 mL·100 g-1·min-1at least in 1 blood supplied region in the vasospasm group (72 blood supply regions altogether) , in which CBF were lower than 25 mL·100 g-1·min-1 in 7 blood supplied regions. Circled digit twoBefore and after the expansion, there were no significant differences among the systolic and diastolic blood pressure, mean arterial pressure, and central venous pressure in the vasospasm group (P >0.05). Circled digit threeOf the 65 vessels with rCBF >25 mL·100 g-1·min-1 there were no significant differences in rCBF before and after the expansion (P = 0.272) ; of the 7 vessels with rCBF ≤ 25 mL·100 g-1·min-1, the rCBF increased from 20 ± 3 mL·100 g-1·min-1to 34 ± 6 mL·100 g-1·min-1. There were significant differences (P <0.01). Circled digit oneOne year follow-up after the procedure, there was no significant difference in GOS score between the 2 groups. Conclusion: Colloidal solution expansion may increase the rCBF of the ischemic regions in patients with cerebral vasospasm and help to improve the prognosis of the patients.