RESUMEN
Objective:To establish an HPLC method for the determination of two components in compound artemisinin analgin tab-lets. Methods:A Waters Symmetry C18 column (250 mm × 4. 6 mm,5 μm) was used with methanol-0. 02 mol·L-1 KH2 PO4 solution (25 ∶75, adjusting pH to 3. 5 with phosphoric acid ) as the mobile phase. The flow rate was 1. 0 ml·min-1 and the detection wave-length was 250 nm. The column temperature was 30℃ and the injection volume was 20 μl. Results:There was a good linear relation-ship when the content of analgin and puerarin was within the range of 50.11-601.36 μg·ml-1(r=0.999 9) and 2.64-31.74 μg· ml-1(r=0. 999 8), respectively. The average recovery was 99. 1%(RSD=0. 7%,n=9)and 99. 9%(RSD=0. 6%,n=9),respec-tively. Conclusion:The method is simple and accurate with good reproducibility,which can be used to control the quality of the prepa-ration.