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AIM: To explore the inhibitory effect and mechanism of compound salvia miltiorrhiza on ovarian cancer by using network pharmacology and molecular docking knowledge. METHODS: The core components of compound salvia miltiorrhiza and the inhibitory effect of compound salvia miltiorrhiza on ovarian cancer cell cycle were studied by combining the methods of MTT cell cycle inhibition and MTT signal network of compound salvia miltiorrhiza. RESULTS: Based on network pharmacology, the core components of compound salvia miltiorrhiza were luteolin and quercetin, and the core target of the disease was VEGFA, SRC, EGFR, hsp90aa1. The docking mode between the core component and the core target EGFR was verified and analyzed by molecular docking. Finally, MTT colorimetry was used to verify that luteolin, one of the core components, significantly inhibited the proliferation of ovarian cancer cells. The results of flow cytometry showed that luteolin induced ovarian cancer A2780 cell cycle arrest in G1/S phase.CONCLUSION: Compound salvia miltiorrhiza preparation can inhibit the proliferation of ovarian cancer cells, which may be related to PI3K Akt signal pathway mediated by EGFR; Network pharmacology and molecular docking technology have important predictability and possibility for the treatment of tumor by compound salvia miltiorrhiza, and have guiding significance for the role and mechanism of compound salvia miltiorrhiza against ovarian cancer cells.
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Objective To investigate the value of butylphthalide and sodium chloride injectio combined with compound salvia miltiorrhiza injection in the treatment of acute ischemic stroke.Methods From January 2016 to December 2017,104 patients with acute ischemic stroke in Tongji Huangzhou Hospital Affiliated to Huazhong University of Science and Technology were randomly divided into observation group (52 cases) and control group (52 cases) according to the digital table.The control group was given butylphthalide and sodium chloride injectio,and the observation group was combined with the compound salvia miltiorrhiza injection on the basis of the control group.The courses of the treatment in the two groups were 14 d.The effects of the two groups were compared.Results The total effective rate of the observation group(90.38％,47/52) was higher than that of the control group(73.08％,38/52) (x2 =5.216,P ＜ 0.05).After treatment,the daily living activity (ADL) score of the observation group was (60.49 ± 5.46) points,which was higher than (44.54 ± 4.10) points of the control group (t =16.845,P ＜ 0.05).After treatment,endothelin-1 (ET-1),C reactive protein (CRP),homocysteine (Hcy) in the observation group were (37.42 ± 5.47) ng/L,(6.51 ± 1.89)mg/L,(7.42 ± 1.27) μmol/L,respectively,which in the control group were (58.98 ± 7.89) ng/L,(12.34 ± 2.65) mg/L,(15.39 ± 2.06) μmol/L,respectively,the levels of ET-1,CRP and Hcy in the observation group were lower than those in the control group (t =16.194,12.916,23.749,all P ＜ 0.05).After treatment,the plasma viscosity,fibrinogen,whole blood viscosity at high shear and low shear whole blood viscosity of the observation group were (1.20 ± 0.23) mPa/s,(2.54 ± 0.37) g/L,(2.54 ± 0.37) mPa/s and (8.19 ± 0.68) mPa/s,respectively,which of the control group were (2.09 ± 0.32) mPa/s,(3.47 ± 0.58) g/L,(3.46 ± 0.59) mPa/s and (9.81 ± 0.82) mPa/s,respectively,the plasma viscosity,fibrinogen,whole blood viscosity at high shear and low shear whole blood viscosity of water of the observation group after treatment were lower than those of the control group(t =16.286,9.748,10.994,10.966,all P ＜ 0.05).Conclusion The effect of butylphthalide and sodium chloride injectio combined with compound salvia miltiorrhiza injection in the treatment of acute ischemic stroke is significant,which is worth studying.
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Objective:To observe the therapeutic efficacy of somatostatin combined with compound salvia miltiorrhiza injection(复方丹参注射液)on patients with acute pancreatitis(AP).Methods:By using randomized controlled trail,62 patients with AP were divided into control group(31 cases) and observation group(31 cases) in a period from June,2002 to December,2006.In the control group,routine measures,such as forbidding food,decompressing stomach and intestines,were taken,and in addition,intravenous injection of somatostatin 250 ?g with subsequent intravenous drip 3 000 ?g in normal saline 500 ml at a rate of 250 ?g/h once everyday was used for 7-14 days.On the above treatment of the control group,compound salvia miltiorrhiza injection 20 ml in normal saline 250 ml,intravenous drip every day for 7-14 days,was added in the observation group.The disappearance of symptoms and abdominal signs,amylase of urine and blood,the time of recovery of hepatic function,change of calcium concentration,incidences of multiple organ dysfunction syndrome(MODS) and case fatality were observed and compared.Results:Amylase of blood after treatment in observation group was lower than that in the control group,while serum calcium concentration was higher,especially on 3 and 5 days after treatment(all P
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AIM: To investigate the effects of compound salvia miltiorrhiza(SM) injection on lung injury after cardiopulmonary bypass(CPB).METHODS: Twenty-four rats were randomly divided into control group,CPB group and SM group.Cardiopulmonary bypass was made in rats of CPB group and SM group.Lung tissues of all rats were taken 60 min after the weaning of CPB for the assay of malondialdehyde(MDA),myeloperoxidase(MPO),water content of tissue, the observation of pathological changes were made under optical microscope.RESULTS: Remarkable pathohistological and biochemical changes were detected 1 h after CPB.Compared with control group,MDA in lung tissue were increased in CPB group and SM group(P
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AIM: To investigate the effect of compound salvia miltiorrhiza pills on nailfold microcirculation of the elderly patients with diabetes mellitus. METHODS: The 38 patients were given, based on the regular treatment of decreasing blood sugar, the compoud salvia miltiorrhiza pills (10 pills, po, tid) for 3 mon. The alteration of nailfold microcirculation were observed before and after the treatment. RESULTS: All kind of parameters and comprehensive integral values of their nailfold microcirculation were improved after the regular treatmeat combined with the compound salvia miltiorrhiza. CONCLUSION: The compound salvia miltiorrhiza pills can improve the nailfold microcirculation of the elderly patients with diabetes mellitus.
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Object To study the effects of aerosol inhalation of Compound Salvia Miltiorrhiza Injection (CSMI) on the sputum rheology properties in respiratory tract of postoperative patients after pneumotectomy.Methods Patients in control group inhaled distilled water by ultrasonic nebulizer, patients in the experiment group inhaled CSMI by the same ultrasonic nebulizer.Results The sputum viscosity on the first, third, fourth, and fifth day postoperation (P