RESUMEN
OBJECTIVE:To establish a method for simultaneous determination of chlorogenic acid,baicalin and quercetin in Compound yuxingcao tablets.METHODS:HPLC method was adopted.The separation was performed on GL InterSustain-C18 column with mobile phase consisted of water-methanol (55∶45,V/V) at the flow rate of 1.0 mL/min.The detection wavelength was set at 270 nm,and the column temperature was 30 ℃.The sample size was 20 μL.RESULTS:Linear ranges of chlorogenic acid,baicalin and quercetin were 1.264-12.64 μg/mL (r=0.999 9),8.704-87.04 μg/mL(r=0.999 9) and 1.212-12.12 μg/mL(r=0.999 9),respectively.The limit of quantification were 1.88,2.98,1.94 ng,respectively.The limits of detection were 0.56,0.89,0.58 ng,respectively.RSDs of precision,stability and reproducibility tests were all lower than 2.0％.The recoveries were 98.02％-101.10％ (RSD=1.01％,n=9),97.48 ％-101.70％ (RSD=1.38％,n=9) and 98.31％-101.10％ (RSD=1.02 ％,n=9),respectively.CONCLUSIONS:The established method is simple,accurate,stable and reproducible,and can be used for simultaneous determination of chlorogenic acid,baicalin and quercetin in Compound yuxingcao tablets.
RESUMEN
OBJECTIVE:To establish a method for simultaneous determination of chlorogenic acid,baicalin and quercetin in Compound yuxingcao tablets.METHODS:HPLC method was adopted.The separation was performed on GL InterSustain-C18 column with mobile phase consisted of water-methanol (55∶45,V/V) at the flow rate of 1.0 mL/min.The detection wavelength was set at 270 nm,and the column temperature was 30 ℃.The sample size was 20 μL.RESULTS:Linear ranges of chlorogenic acid,baicalin and quercetin were 1.264-12.64 μg/mL (r=0.999 9),8.704-87.04 μg/mL(r=0.999 9) and 1.212-12.12 μg/mL(r=0.999 9),respectively.The limit of quantification were 1.88,2.98,1.94 ng,respectively.The limits of detection were 0.56,0.89,0.58 ng,respectively.RSDs of precision,stability and reproducibility tests were all lower than 2.0％.The recoveries were 98.02％-101.10％ (RSD=1.01％,n=9),97.48 ％-101.70％ (RSD=1.38％,n=9) and 98.31％-101.10％ (RSD=1.02 ％,n=9),respectively.CONCLUSIONS:The established method is simple,accurate,stable and reproducible,and can be used for simultaneous determination of chlorogenic acid,baicalin and quercetin in Compound yuxingcao tablets.
RESUMEN
To study the quality of Lonicerae iaponicae flos in compound Yuxingcao tablets. Methods: HPLC-ELSD and LC-MS/MS were used to determine Lonicerae flos using macranthoidin A, macranthoside B and dipsacoside B as the control. Re-sults:HPLC-ELSD was suitable for the detection of Lonicerae flos in compound Yuxingcao tablets. LC-MS/MS was used to verify the results, which could identify the certified or counterfeit Lonicerae iaponicae flos in the samples. Conclusion:The two methods can be used to control the quality of Lonicerae iaponicae flos in compound Yuxingcao tablets.