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Objective:To establish an FPLC-MS/MS method for the determination of fluvoxamine in human plasma. Methods:The separation was performed on an Inertsil? ODS-SP column(2. 1 × 100 mm, 3 μm). The mobile phase was acetonitrile-2 mmol· L-1 ammonium acetate (45: 55, v/v) containing 0. 1% formic acid at a flow rate of 0. 3 ml·min-1 . Lansoprazole was used as the internal standard( IS) . Electrospray ionization ( ESI) source was applied and operated in a positive ion model. Multiple reaction moni-toring (MRM) model with the transitions of fluvoxamine m/z 319. 1→m/z 69. 8 and lansoprazole m/z 370. 2→m/z 252. 1 was used to quantify fluvoxamine and IS, respectively. Results:In human plasma, the standard curve was linear within the range of 1-100 μg· L-1 . The lower limit of quantification of fluvoxamine( LLOQ) was 1μg·L-1 . The intra-day RSD was less than 5%, the inter-day RSD was less than 10%, and the method recovery was 85%-95%. Conclusion:The method is simple, sensitive, accurate and reproduci-ble. It is applicable in the pharmacokinetic study of fluvoxamine for clinical pharmacokinetics and bioequivalence studies.
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Objective:To establish an FPLC-MS/MS method for the determination of fluvoxamine in human plasma. Methods:The separation was performed on an Inertsil? ODS-SP column(2. 1 × 100 mm, 3 μm). The mobile phase was acetonitrile-2 mmol· L-1 ammonium acetate (45: 55, v/v) containing 0. 1% formic acid at a flow rate of 0. 3 ml·min-1 . Lansoprazole was used as the internal standard( IS) . Electrospray ionization ( ESI) source was applied and operated in a positive ion model. Multiple reaction moni-toring (MRM) model with the transitions of fluvoxamine m/z 319. 1→m/z 69. 8 and lansoprazole m/z 370. 2→m/z 252. 1 was used to quantify fluvoxamine and IS, respectively. Results:In human plasma, the standard curve was linear within the range of 1-100 μg· L-1 . The lower limit of quantification of fluvoxamine( LLOQ) was 1μg·L-1 . The intra-day RSD was less than 5%, the inter-day RSD was less than 10%, and the method recovery was 85%-95%. Conclusion:The method is simple, sensitive, accurate and reproduci-ble. It is applicable in the pharmacokinetic study of fluvoxamine for clinical pharmacokinetics and bioequivalence studies.
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AIM:To study the effect of berberin hydrochloride(Ber)on the elevation of blood concentra- tion of Cyclosporin A(CsA)and liver and renal functions in renal transplanted recipients.METHODS:138 cases with renal transplantation in experimental group were treated with CsA and Ber and 150 cases with renal trans- plantation in control group received CsA only.Blood lev- els of CsA,biochemistry indexes for liver and renal func- tion were determined.RESULTS:After 1,3,6 months combined with Ber,blood concentrations of CsA in pa- tients went up by 49.8%,81.4%,36.9% and the radios of blood concentration of CsA to CsA dosage increased 88.8%,102.0%,72.5% compared with those before taking Ber(P
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OBJECTIVE:To evaluate the rationality of vancomysin use in the hospital where the authors work.METH-ODS:50patients taking vancomysin between May.2005and Nov.2005were studied.The pathogenic examination,monitoring of drug concentration in blood,indices of renal function,combined use of drugs as well as adverse effects in these patients were reviewed and analyzed statistically.RESULTS:On the whole vancomysin use in our hospital was safe.CONCLUSION:Vancomysin use will be made safer by monitoring drug concentration in blood,individualizing drug administration,and timely correcting the aggravating factors of kidney injury.
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OBJECTIVE:To study the anti-infective effect of ceftriaxone in perioperative period METHODS:28 cases of cholelithiasis complicating infections who had to undergo laparoscopic cholecystectomy were enrolled in this study Ceftriaxone,1g,im,was injected 1h～1 5h before anesthesia,and blood and bile were sampled before,during and after operation The concentration of ceftriaxone was determined by UV-spectrophotometry RESULTS:The concentrations of ceftriaxone in blood before,during and after operation were(37 59?16 13)?g/ml,(49 68?10 51)?g/ml and (27 09?12 38)?g/ml respectively The drug concentration in blood peaked 2h after drug administration,at this time,the drug concentration of bile was 4 times that of blood (201 77?20 16)?g/ml CONCLUSION:The drug concentration in blood was 49 times as high as MIC,which indicates that ceftriaxone has preventive and therapeutic effects on possible infections during perioperative period
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OBJECTIVE: To establish a HPLC assay for determining cassiaside B in rabbit plasma .METHODS: The proteinin sample was first denatured with methanol .The ?-- Bondapak C_(18) colurnn(3.9mm?300mm, 10?m) was used with a mobilephase of acetonitrile -- water -- THF -- glacial acetic acid(20:76.5:3.0:0.5) .The flow rate was 1.0ml/ min .Detecting wave-length was 278nm .RESULTS: The caliblation curve of cassiaside B was C=9.102?10~(-2)+4.871?10~(-5)R(r=0.9999) .Theaverage recovery of cassiaside B in plasma was (100.8?1.139)% .The relative standard deviations of intra -- day and inter -- dayassay were less than 2.08% .The detection limit in plasma was 0.05?g/ml .CONCLUSION: The method is sensitive and accu-rate. It is suitable for the pharmacokinetic study of cassiaside B.
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OBJECTIVE:To analyse the correlation between cyclosporine A(CsA)and the biochemical indicator of renal transplantation patients.METHODS:The patients were divided into6groups according to the different time length after renal transplatataion:0y～1y,1y～2y,2y～3y,3y～4y,4y～5y and above5y;the blood samples were taken12h after oral administra?tion of CsA,the of concentration CsA in blood of each group was determined and the biochemical tests of which were taken;post-operation medication and the correlation between the blood biochemical index were statistically analyzed by Stata soft?ware.RESULTS:The dosage of CsA decreased gradually from group1to group6,and the concentration of CsA also de?creased.And the data of BUN in group1,5and6were higher than the upper limit of their standard data,the data of triglyc?eride(TG)in group1,2,3,5and6were higher than the upper limit of their stand data;there were significant differences in the data of TP、ALB、TB、DB、Ua、TC、TG、?-GT among the6groups,while the data of ALP、ALT、AST、Glu、BUN、Cr among the6groups have no notable difference.CONCLUSION:The administration of CsA after renal transplantation can change bio?chemical indicator in patients.
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The toxicity of HCN was determined first.The experimental animals were intoxicated with a HCN concentration of 1103.8?48.32 Y/L and were exposed for 4.55?2.04 minutes.The mortality rate in 24 hrs was recorded.The LDEO,sLD50,and LD99 were calculated as 844.31?/kg,172.39?/kg and 959.18?/kg respectively.Two preparations of DMAP injection were found to have preserved rather good anti-HCN potency even though they had been kept under room temperature for about one year.All the 11 dogs survigd a dose of 4 LD50 of HCN in halation poisoning when DMAP was injected intramuscularly 1 minute after exposure.Another 5 dogs inflicted with similar HCN intoxication were also all saved when intravenous DMAP injection was given at the time of respiratory arrest. The cyanide level in the blood of the dogs after inhalation of HCN was determined by spectrophotofluorometry with pyridoxal.The blood concentration after inhalation of a lethal dose of HCN was 110?26.6?/100 ml at the moment of respiratory arrest.DMAP after intramuscular injection can shift the cyanide combined with cytochrome oxidase to red blood cells to form ferrihemoglobin cyanide,and cell respiration is then restoredEventually the dogs could survive the 4 LD50 HCN intoxication.