RESUMEN
Condyloma acuminatum is an uncommon disease but there is an increased incidence reported in recent years in prepubertal children. Anal and perianal condyloma accuminatum in children may suggest sexual abuse and treatment should include the thorough social evaluation as well as medical treatment. A 25 month old girl presented with multiple sessile nodules around her anal and perianal area, Biopsy confirmed the diagnosis of condyloma accuminatum. There was no definite evidence of sexual abuse, but her father had condyloma accuminatum involving his penis. The patient's lesions were excised totally. At three week follow up there was no anal stricture but there were newly developed small lesions around perianal area. Imiquimod onit was applied for 2 weeks and the recurred lesion disappeared completely. At postoperative 5 month, the operative area was fully epithelialized without recurrence.
Asunto(s)
Niño , Humanos , Masculino , Aminoquinolinas , Biopsia , Constricción Patológica , Padre , Estudios de Seguimiento , Incidencia , Pene , Recurrencia , Delitos SexualesRESUMEN
Objective To construct an expression plasmid of human papillomavirus type 11 E7 (HPV11-E7)/hurnan IFN?-2b fusion gene, to express the fusion gene in E.coli BL21, and pave way for further immunological study. Methods The recombinant plasmid was introduced into E.coli BL21, then the expression product was analyzed by SDS-PAGE and Western blotting after induction with isopropy-?-D-thiogalactoside (IPTG). Results The fusion gene of HPV11-E7 and human IFN?-2b was successfully cloned into pET-32a by a linker with the same sequence as we expected. The expressed fusion protein was confirmed by SDS-PAGE and Western blotting. Conclusions The successful construction of prokaryotic expression plasmid and expression of HPV11-E7/human IFN?-2b fusion gene enable further immunological study.
RESUMEN
Lymphangioma circumscriptum is an uncommon benign hamartomatous malformation that consists of dilated lymphatic channels lined by normal, single-cell lymphatic endothelia. The diagnosis of lymphangioma circumscriptum is usually made on the basis of clinical characteristics. However, a biopsy is required to exclude other clinically resembling diseases, especially when the lesion shows unusual clinical features. We report a case of recurred perianal lymphangioma circumscriptum in a 13-year-old boy, which showed a verrucous mass having features of condyloma accuminatum.
Asunto(s)
Adolescente , Humanos , Masculino , Biopsia , Diagnóstico , LinfangiomaRESUMEN
Objective:To investigate the changes in number,morphology and function of Langerhans cells and the molecular mechanisms in the tissues of condyloma accuminatum.Methods:CD1a,E-cadherin were measured by streptavidin peroxidase method and reverse transcriptase PCR in 26 CA lesions and 10 normal controls.Ultramicrostructure of LC was observed by transmission electronic microscope(TEM).Results:In CA tissues,the number of CD1a+ LC decreased(10.66?11.71,P