RESUMEN
Guanylate binding protein-1(GBP-1) is an interferon-induced protein. To observe its antiviral effect against Hepatitis B virus (HBV) and Coxsackie virus B3 (CVB3), we constructed an eukaryotic expression vector of human GBP-1(hGBP-1). Full-length encoding sequence of hGBP-1 was amplified by long chain RT-PCR and inserted into a pCR2.1 vector, then subcloned into a pCDNA3.1(-) vector. Recombinant hGBP-1 plasmids and pHBV1.3 carrying 1.3-fold genome of HBV were contransfected into HepG2 cells, and inhibition effect of hGBP-1 against HBV replication was observed. Hela cells transfected with recombinant hGBP-1 plasmids were challenged with CVB3, and viral yield in cultures were detected. The results indicated that recombinant eukaryotic expression plasmid of hGBP-1 was constructed successfully and the hGBP-1 gene carried in this plasmid could be efficiently expressed in HepG2 cells and Hela cells. hGBP-1 inhibit CVB3 but not HBV replication in vitro. These results demonstrate that hGBP-1 mediates an antiviral effect against CVB3 but not HBV and perhaps plays an important role in the interferon-mediated antiviral response against CVB3.
RESUMEN
OBJECTIVE:To investigate the antiviral action of emodin in combination with other Chinese active medicine components on Coxsackie virus B3(CVB3) and explore the possible antiviral mechanism.METHODS:Hep-2 cells infected by CVB3 were cultured with different concentration and proportion of the emodin and other Chinese medicine components for 72 hours and their inhibitory effects on CVB3 replication were evaluated with cell survival rates by MTT assay.RESULTS:In the Hep-2 cell system,all the combinations between emodin and other medicine components could inhibit cytopathic effect(CPE) of CVB3-infected cells,increase cells' survival rates and decrease the cytotoxicity.The drug combination which showed the best effect of killing viruses directly was emodin:curcumine:banlangen(2:1:2),and the one showing the highest efficacy in inhibiting the replication of CVB3 in cells was emodin:curcumine:banlangen(2:0:1).CONCLUSION:The toxicity of emodin was reduced and its anti-CVB3 activity was enhanced by its combined use with other Chinese active medicine components.