Experimental research on culturation, cryopreservation and thawing with sandwiched-cultured rat hepatocytes between two collagen gel layers / 介入放射学杂志

Objective To evaluate the effects of double collagen and epidermal growth factor (EGF) on morphology, viability and function of Sprague-Dawley (SD) rat hepatocytes after cryopreservation and thawing. To investigate a kind of more ideal liver cell cultivation in situ, for offering the most basic material in liver cell transplantation.Methods Rat hepatocytes were isolated by a two-step collagenase technique and cultured in different conditions. After freeze-thaw-cycle, the viability and function of the cells were measured. Results The viability and function of hepatocytes in collagen and in collagen with EGF groups were prior to those in other groups. ( P