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Objective To establish the qualitative and quantitative analytic method for quality control of Wujin capsules.Methods Curcuma wenyujin Y.H.Chen et C.Ling and Crataegus pinnatifida Bge.in Wujin capsules were identified by TLC method.The content of tenacissoside H was determined by HPLC.Chromatographic separation was achieved at Waters Xbridge C18 column (100 mm× 3.0 mm, 3.5 μm) with an evaporative light scattering detector (ELSD).Acetonitrile and H2O were used as the mobile phase for isocratic elution.Results The qualitative identification by TLC was specific.The well separated clear spots were exhibited on each thin layer plate.Tenacissoside H had a linear equation in the range of 11.60-580.00 μg/ml(r=0.999 0).The average recovery was 101.54％.Conclusion The TLC identification of Curcuma wenyujin Y.H.Chen et C.Ling and Crataegus pinnatifida Bge.was simple and well established.The HPLC-ELSD method was specific,sensitive and reliable.Those methods can be used for the quality control of Wujin capsules.
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Objective: To research on the most appropriate SRAP-PCR amplication system of Curcuma wenyujin. Methods: The concentration of Mg2+, dNTP, template DNA, primer, and Taq polymerase was optimized by amplifying technology and method of PCR based on isolating genomic DNA from C. wenyujin. Results: The best amplification system for C. wenyujin was as follows: total reaction volume of 25 μL, including Mg2+ (25 mmol/L) 2.0 μL, Taq polymerase(5 U/μL)0.4 μL, random primer (20 μmol/L) 2 μL, template DNA (5 ng/μL) 2.0 μL, dNTP (25 mmol/L) 2.0 μL, 10 × PCR buffer 2.5 μL. Conclusion: The stripes of amplification products are clearness, high brightness, and good reproducibility. It indicates that the reaction system and reaction procedures which are confirmed in this experiment are applied to the SRAP molecular markers in C. wenyujin, based for the genetic diversity of C. wenyujin.
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[Objective]To discuss the analgesic effects of Radix Curcuma extracts on mice.[Method]The writhing tests were adopted to observe the mice writhing times.[Results]The Radix Curcuma extracts middle-dose group can obviously inhibit the mice average writhing times,with statistically significant differences in comparison to contral group,P
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Objective To establish the chromatographic fingerprint of Curcuma wenyujin by HPLC.Methods The chromatographic fingerprint of C.wenyujin was established by HPLC with Shimadzu VP-ODS C18 column(250 mm?4.6 mm,5 ?m),acetonitrile-water(0.1 phosphoric acid) gradient elution,the flow rate of 1.0 mL/min,column temperatures of 30 ℃,and detective wavelength at 210 nm.ResultsTaking germacrone as the reference peak,14 common-peaks were selected as the fingerprint peaks.The similarities among samples of C.wenyujin among 19 approved samples and their standard chromatographic fingerprints were calculated with a software "Similarity Evaluation System for Chromatographic Fingerprint of Chinese Materia Medica" published by GPC(Version 2004A) and cluster analysis.ConclusionThis method with better accuracy and reproducibility could be used in the quality control of C.wenyujin.