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OBJECTIVE: To explore the anti-tumor and immunologic enhancement effects of Xiangling capsule.METHODS: The tumor-inhibition experiment was conducted in vivo in accordance with the screening criteria of anti-tumor drugs.Mice were treated with cyclophosphamide to induce hypoimmunity model.The effect of Xiangling capsule on mice was tested by determining leukocyte count,thymus index,spleen index,and the activity of NK cells.RESULTS: Xiangling capsules at different concentration showed inhibition on the growth of mice-transplanted tumor S180,H22 hepatomas and Lewis lung carcinoma with inhibition rates of 44.8%,52.2 %,and 43.3%,respectively(P
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OBJECTIVE:To explore the effects of ERC61,an extract from Rhizoma Curcumae on hematopoietic functions of mice pretreated by cyclophosphamide(CTX) and the proper regime of the administration of ERC61.METHODS:The experimental mice were treated with CTX(100mg? kg-1? d-1) by ip for 3 consecutive days to establish leucopenia model.ERC61 was ip administered to the prevention group,the treatment group,and the combined group(prevention+ treatment).The numbers of blood leucocytes and bone marrow cell were counted by conventional methods;spleen index was measured as well.RESULTS:ERC61 enhanced the count of leucocytes and nucleated cells in bone marrow and improved the level of spleen index.CONCLUSION:ERC61 is able to improve the disorders of hematopoietic functions induced by CTX,and the combination therapy of prevention and treatment is the optimum one in efficacy.
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Objective To study the effect of low dose cyclophosphamide on function of CD4+CD25+Treg of mice with Lewis lung cancer and investigate the relationship between CD4+CD25+Treg and the genesis and development of tumor.Methods The models were divided into three groups:CTX group,tumor group and control group.In CTX group,models were established by injection CTX(25 mg?kg-1) and subcultivated Lewis lung cancer cells were injected subcutaneously to the right axilla of C57BL/6 mice 7 d later.The dynamic changes of tumor volume were observed.The changes of the number of CD4+CD25+Treg were detected by flow cytometer and the expression of Foxp3 mRNA in spleen was analyzed by semi-quantitative RT-PCR.The changes of T lymphocyte proliferation in spleen were detected by MTT test.The changes of T lymphocyte killing function in spleen were detected by LDH releasing test.Results Compared with tumor group,CTX treatment might delay the development of tumor in 19 d-mice with Lewis lung cancer.The number of CD4+CD25+ Treg in spleen of mice in CTX group was lower than that in tumor group(P0.05).Conclusion After CTX injection,the number of CD4+CD25+Treg and the expression of Foxp3 mRNA in spleen of mice with Lewis lung cancer decrease,the immunological function of mice with Lewis lung cancer increase.It can provid experiment evidence for the tumor immunotherapy aimed directly at CD4+CD25+Treg.