Role of IL-17 in nucleus pulposus cell proliferation and metabolism culturedin vitro

Objective:To explore the role of cytokine, interleukin-17(IL-17) in human degenerative disc disease.Methods:Through magnetic resonance imaging, human degenerative disc tissues were confirmed from the isolated nucleus pulposus cells, which were then cultured in vitro.The cells were cultured with and without different concentrations ofIL-17.2 ng/mL,5 ng/mL,10 ng/mL,15 ng/mL and20 ng/mLIL-17 concentrations were used for stimulation.After72 hours, the inhibition rate of proliferation was measured byMTS method.For48 and96 hours, the nucleus pulposus cells were cultured with and without the appropriateIL-17 concentrations.The mRNA and protein expression levels of the matrix macromolecules and degrading tissue genes were measured byReal-timePCR andWestern blot analysis.Results:It was noted that nucleus pulposus cell proliferation was inhibited after culturing in vitro withIL-17 stimulation, and it was further observed that the inhibition effect was significantly stronger with15 ng/mLIL-17 concentration.With the dosage of15 ng/mL,IL-17 stimulation induced multiple cellular responses, such as the significant increase in mRNA expression for both aggrecan(ACAN) and type Ⅰ collagen(COL1A1) genes(P<0.05), and the significant decrease in mRNA expression of both degrading tissue genes,MMP3 andTIMP3(P<0.05).Western blot results also showed that the protein level ofCOL1A1 was significantly decreased(t=3.199,P=0.006), while the protein level of one peptidases(ADAMTS5) significantly increased(t=2.667, P=0.021).Conclusions:These findings suggest thatIL-17 can inhibit proliferation and affect the metabolism of the cultured nucleus pulposus cells in vitro, and these findings could possibly contribute to the degenerative changes that occur inDDD through extracellular matrix synthesis inhibition, promoting nucleus pulposus extracellular matrix degradation and disrupting the metabolic balance.

Case Study of Hepatic Radiofrequency Ablation Causing a Systemic Inflammatory Response Under Total Intravenous Anesthesia

OBJECTIVE: To investigate the effects of hepatic radiofrequency ablation (RFA) in patients with malignant liver disease with respect to inflammation activation and stress response. MATERIALS AND METHODS: In an observational trial, we investigated the physiologic parameters of 17 patients (20 interventions) who underwent percutaneous RFA under general anesthesia after applying total intravenous anesthesia. TNFalpha, IL-6, IL-8, IL-10, adrenaline and noradrenaline, liver enzymes, lactate and creatine kinase were determined pre-interventionally after induction of anesthesia (T1), 90 minutes after initiation of RFA (T2), immediately after the conclusion of the procedure (T3), and 24 hours after the procedure (T4). RESULTS: A significant increase in body temperature (p < 0.001), and mean arterial pressure (p = 0.001) were measured intraoperatively (T2) and the day after the procedure (T4). Increased levels of IL-6 were measured at T3 and T4 (p = 0.001). IL-10 increased immediately after the procedure (T3; p = 0.007). IL-6 levels correlated well with the total energy applied (r = 0.837). Significant increases in the levels of adrenaline and noradrenaline were present at T3 and T4 (p < 0.001). The RFA-induced destruction of hepatic tissue was associated with increased levels of AST, ALT, GLDH and LDH. CONCLUSION: Percutaneous RFA of hepatic malignancies causes an inflammatory and endocrine activation, similar to the systemic inflammatory response syndrome. These effects have to be taken in account when dealing with patients susceptible to sepsis or multi-organ failure.

Level and Significance of Inflammatory Cytokines in Patients with Acute Deep Venous Thrombosis of Lower Extremity / 中国普外基础与临床杂志

To study the significance of the levels of plasma inflammatory cytokines (IL-6,IL-8,IL-10 and TNF-?) in patients with acute deep venous thrombosis (DVT) of lower extremity. Methods Forty untreated DVT cases were selected as the subjects in the DVT group, while thirty healthy subjects, whose ages and genders showed no significant difference with the DVT patients, were collected as the control group. The plasma levels of IL-6, IL-8 and TNF-? were detected by radioimmunoassay (RIA), and the plasma level of IL-10 was measured by enzyme-linked immunosorbent assay (ELISA). Correlation analysis was used to investigate the relationships between the levels of different inflammatory cytokines within DVT group. Results The levels of plasma cytokines in the DVT group were all significantly higher than those in control group (P