RESUMEN
OBJECTIVE:To observe the effect of2,3,5,4'-tetrahydroxy stilbene—2—?—D—glycoside(TSG)on learning and memory ability and neurotrophic factors of dementle model mice induced by D-galactose.METHODS:The mice were randomly divided into normal control,D-galatose model,VitE positive control,TSG low dose(0.033g/kg),TSG medium dose(0.1g/kg),TSG high dose(0.3g/kg)groups.The mice of various therapy groups excluding the normal Control group were in?jected with D-galactose(D-gal)solution s.c.(50mg/kg/day)over a60-day period,while normal control was injected with saline.At the same time,therapy groups were given TSG and positive control VitE(0.08g/kg)a day,and all therapy groups were administered by intragastrically for60days.Then all mice of different groups were tested with Morris water maze test,and five mice of each group were killed and the expression of nerve growth factor(NGF)and neurotrophin-3(NT-3)was determined with immunohistochemistry method.RESULTS:Injection of D-galactose for2months induced the learning and memory dysfunction of mice,and abated NGF and NT-3expression in hippocampal neurons.TSG improved the learning and memory ability of D-gal model mice,promoted NGF and NT-3expression in hippocampal neurons.CONCLUSION:SG can improve memory ability obviously and may prevent and cure dementia disease such as AD.