Anti-Oxidative Effect of Myrtenal in Prevention and Treatment of Colon Cancer Induced by 1, 2-Dimethyl Hydrazine (DMH) in Experimental Animals

Colon cancer is considered as the precarious forms of cancer in many developed countries, with few to no symptoms; the tumor is often diagnosed in the later stages of cancer. Monoterpenes are a major part of plant essential oils found largely in fruits, vegetables and herbs. The cellular and molecular activities show therapeutic progression that may reduce the risk of developing cancer by modulating the factors responsible for colon carcinogenesis. Colon cancer was induced with DMH with a dose of (20 mg/Kg/body weight) for 15 weeks by subcutaneous injection once in a week. Myrtenal treatment was started with (230 mg/Kg/body weight) by intragastric administration, one week prior to DMH induction and continued till the experimental period of 30 weeks. The Invivo results exhibit the elevated antioxidant and lipid peroxidation levels in DMH treated animals. The Histopathological analysis of colon tissues well supported the biochemical alterations and inevitably proves the protective role of Myrtenal. Treatment with myrtenal to cancer bearing animals resulted in a remarkable increase in the inherent antioxidants and excellent modulation in the morphological and physiological nature of the colon tissue. It is thus concluded that myrtenal exhibits excellent free radical scavenging activity and anticancer activity through the suppression of colon carcinoma in Wistar albino rats.

Anticancer activities of mushroom polysaccharides on chemically-induced colorectal cancer in rats.

Polysaccharides from mushroom Pleurotus sajor-caju (PS1) and Lactuca Sativa (PS2) were isolated and purified (22.40 and 26.80g/100g respectively). Cytotoxic activities of PS1 and PS2 were examined In vitro using colon (HCT 116), liver (HEPG2), cervical (HELA) and breast (MCF7) carcinoma cell lines. The present results indicated that these polysaccharides (PS1 and PS2) have more inhibitory effects on HCT-116 than the other carcinoma cell line (HEPG2, HELA and MCF7). Polysaccharides (PS1 and PS2)-treated HCT-116 cells showed a high percentage of cell death, indicating promising anti-tumorigenic properties, and demonstrate their direct effect on colon cancer cell proliferation. Evaluation of the two polysaccharides (PS1and PS2) was carried out through determination of the tumor markers (CEA and C19.9) in cancer rat groups treated with either PS1 or PS2 compared versus carcinogenic control rats. Polysaccharides (PS2) administration caused higher inhibitory effect on chemically-induced colorectal cancer in rats through the histological examination and a marked reduction in the levels of ALP and ALT more than that of PS1 in serum of rats. It can be concluded that polysaccharides (PS1 and PS2) are more effective for inhibition of chemically induced colorectal cancer in rats.

Decrease of intestinal tumors induced by 1,2-dimethylhydrazine in rats fed with cow milk and buffalo milk

Epidemiological studies in human beings and experimental studies in laboratory animals suggest that milk and dairy products can inhibit effects on the development of some kinds of tumors. Cow milk contains sphingomyelin, butyric acid, conjugated linoleic acid, calcium, vitamin A, carotene and vitamin D. All of these components are known to inhibit the process of carcinogenesis. Our objective was to determine the effect of cow milk and water buffalo milk on the development of colon neoplasias in an experimental model of carcinogenesis in rats induced with 1,2-dimethylhydrazine (DMH). Three-month-old Wistar male rats with an average body weight of 180 g were given a nutritionally adequate diet and drinking water adlivitum, cow milk or water buffalo milk. The milk diets were provided two weeks before the first DMH treatment and their administration was continued during the 10 weeks of DMH treatment. Milk administration finished two weeks after the last DMH doses treatment. Four months after the last carcinogen injection, all surviving animals were sacrificed and examined for intestinal tumors. The number, size, and location of the tumors were recorded and gross pathology was described. Small tumors (< 2.5 mm) were examined by Scanning Electron Microscopy (SEM). Significantly fewer tumors were observed in both groups treated with DMH and supplemented with milk, than in the group treated with DMH without milk administration.

The Antitumor Effects in vitro and Impact on Colon Cancer in Rats of Endostatin Transfected Bifidobacterium Oral Powder Preparation / 医学研究杂志

Objective To study the antitumor effects in vitro and impact on colon cancer in rats of endostatin transfected bifidobacterium oral powder preparation(ETB-2).Methods Growth inhibitory effect of the cecropins on normal human gastric epithelial cell line(GES-1) and human colon adenocarcinoma cell line(LS-174T) was observed using a microculturetetrazolium(MTT) colorimetric methods.Male Wistar rats were divided into 4 groups.All treatments were completed in a course of 18 weeks and the experiment was finished at week 33.Results The cecropins showed selective cytotoxic activity against the human colon adenocarcinoma cell line.There was a significant lower in incidence of colon tumors in rats(70%vs100%,P0.05).Conclusion ETB-2 has significant preventive effect on colon cancer induced by DMH in rats.