Evaluation Of In Vitro Antioxidant And Cytotoxic Effect Of Methanol Extract From Aerial Parts Of Myristica Beddomei King Ssp. Ustulata W.J. De Wilde

Plants are traditionally used for pharmacological activities because of its ability to produce bioactive compounds. Myristica beddomei King ssp. ustulata W.J. de Wilde is an ethnomedicinal plant and it is seen in South Western Ghats of Kerala, India. The present study assessed the phenolic content, flavonoid concentration, in vitro antioxidant and cytotoxic effect of different parts of Myristica beddomei King. The total phenolic contents in the extracts ranged from 96.29 (pericarp) to 314.47 (bark) mg g-1 gallic acid equivalent. The concentration of flavonoids in different plant part extracts ranged from 1.81 to 2.76 mg g-1 equivalent to quercetin. All the parts exhibited potential antioxidant activity with an IC50 value of 2.87 to 9.67 μg ml-1 when compared to the standard ascorbic acid with an IC50 value of 2 μg ml-1 in 1,1-diphenyl-2- picryl-hidrasil (DPPH) method. Bark showed highest activity in terms of DPPH radical scavenging (IC50 value of 2.87 µg ml-1), phosphomolybdenum test (2261.33 ± 1.65 mg g-1 trolox equivalent) and ferric ion reducing antioxidant power (FRAP) (113.1 ± 0.28 µmol Fe2+ µg-1) while pericarp showed low antioxidant activity. The in vitro screening results revealed that the seeds exhibited promising anticancer activity compared to PA1 (Ovarian Cancer) cells (50 % inhibition) were observed at a concentration 100.68 µg ml-1. In cytotoxicity test L929 (Fibroblast) cell line compared to the other parts pericarp, mace and seed needed higher concentration (>240 µg ml-1) for LC50 value. It is a promising plant for further development of antioxidant agent as it got high content of phenolic compounds and potential antioxidant and anticancer activity.

Antioxidant Properties, Secondary metabolites and Growth as affected by application of Putrescine and Moringa leaves extract on Jojoba plants.

This work aimed to study the effect of putrescine at (50, 100 and 200 ppm ) as a chemical growth regulator , moringa leaves extract ( 3.5%, 7% and 10 %) as a natural extract and control (distilled water ) on growth, photosynthetic pigments, phytochemicals and antioxidant capacity of jojoba plants grown in earthenware pots. Application of MLE (10 %) caused an increase in plant height by 103.24%, meanwhile (7 %) of MLE led to 4.08% increment in the branches number as compared to control plants. Chlorophyll a, b and carotenoids recorded the highest concentrations by treating with MLE (10 %) followed by putrescine (50 ppm).Total phenolic, flavonoid , tannin content and antioxidant activity of jojoba plants significantly increased with putrescine treatment at 50 ppm and these increments were 28.98%, 31.33%, 74.82% and 65.44%, respectively as compared to control plants. Also, foliar spray of MLE (7 %) led to highest increment of total phenolic, flavonoid and tannins (41.67%, 85.13% and 80.50%, respectively).Moringa leaves extract had the superiority in increasing reducing power ability it increased gradually by increasing moringa leaves extract concentration. Some changes in amino acids concentration were observed with putrescine (50and200 ppm) as well as total essential amino acids.

In vitro antioxidant and antibacterial activities of two fresh water Cyanobacterial species, Oscillatoria agardhii and Anabaena sphaerica.

Cyanobacteria are potential sources of biologically active compounds with antiviral, antibacterial, antifungal, and anticancer activities. In the present investigation, the effect of different solvents, including methanol, acetone, and water on the total phenolic, flavonoid, antioxidant and antibacterial activities of Oscillatoria agardhii and Anabaena sphaerica extracts were evaluated. The results showed that solvents with different polarities have various effects on phenolic content and antioxidant activity. Among the tested solvents, methanolic extract of Oscillatoria agardhii showed the highest antioxidant activity as well as the highest phenolic content. Organic extracts (with methanol or acetone) of the tested species actively inhibited the growth of bacteria compared to aqueous extracts. The highest antibacterial activity was detected by acetone extract of Oscillatoria agardhii against Salmonella senftenberg. By GC-MS analysis some important heterocyclic compound were identified in both Oscillatoria agardhii and Anabaena sphaerica crude extracts. This appeared to be responsible for such excellent antioxidant and antibacterial activity together with other unidentified compounds. Further exploration of antibacterial potential of cyanobacteria can open new horizons.

Antioxidant capacity and total phenolic contents in hydrophilic extracts of selected Bangladeshi medicinal plants

OBJECTIVE@#To evaluate hydrophilic extracts from edible portions of fifteen plants for total phenolic content (TPC) and anti-oxidant capacity (AC) as an effort to find possible sources for future novel antioxidants.@*METHODS@#Folin-Ciocalteau and DPPH radical scavenging assays were employed to determine TPC and AC, respectively.@*RESULTS@#Among the assayed plants, TPC (mean±SD), expressed as gallic acid equivalent, varied from 0.04±0.01 (Amaranthus spinosus) to 6.01±0.04 (Zanthoxylum rhetsa) mg gallic acid equivalent/g fresh weight. AC (mean±SD), expressed as trolox equivalent, ranged from 0.14±0.00 (Alternanthera philoxeroides) to 7.54±0.00 (Zanthoxylum rhetsa) μmol trolox equivalent/g fresh weight. A significant and positive linear relationship (R(2)=0.99) was observed between TPC and AC of Zanthoxylum rhetsa, Oxalis corymbosa, and Alternanthera sessilis.@*CONCLUSIONS@#The results of the present study implies that the analyzed plants possess varying degree of antioxidant capacity and, therefore, the antioxidant potency of these underused plants may be utilized to prevent oxidative damage and oxidative stress related disorders.

Antioxidant activity and free radical scavenging activities of Streptomyces sp. strain MJM 10778

OBJECTIVE@#To investigate the antioxidant activity of soil-borne actinobacteria.@*METHODS@#The total phenolic contents, the level of antioxidant potential by DPPH radical scavenging activity, NO scavenging activity, and ABTS radical scavenging activity in ethyl acetate extract were determined.@*RESULTS@#The 16S rDNA sequencing analysis revealed that Streptomyces sp. strain MJM 10778, which was isolated from Hambak Mountain, Korea, has 99.9% similarity to Streptomyces misionensis (S. misionensis) NBRC 13063. The physiological and the morphological test revealed that the strain MJM 10778 has different characteristics from the strain NBRC 13063. The entire antioxidant assay with the ethyl acetate extract displayed good radical scavenging activity. The IC50 values of the strain MJM 10778 extract on DPPH, NO, and ABTS radicals were identified to be 92.8 μg/mL, 0.02 μg/mL, and 134.9 μg/mL, respectively. The ethyl acetate extract of the strain MJM 10778 showed an 81.50% of cell viability at 100 μg/mL in Raw264.7 cell viability assay.@*CONCLUSIONS@#The results obtained suggest that the ethyl acetate extract of Streptomyces sp. strain MJM 10778 could be considered as a potential source of drug for the diseases that is caused by free radicals with its anti-oxidant activities and low cytotoxicity.

Antioxidant activity and free radical scavenging activities of Streptomyces sp. strain MJM 10778

Objective: To investigate the antioxidant activity of soil-borne actinobacteria. Methods: The total phenolic contents, the level of antioxidant potential by DPPH radical scavenging activity, NO scavenging activity, and ABTS radical scavenging activity in ethyl acetate extract were determined. Results: The 16S rDNA sequencing analysis revealed that Streptomyces sp. strain MJM 10778, which was isolated from Hambak Mountain, Korea, has 99.9% similarity to Streptomyces misionensis (S. misionensis) NBRC 13063. The physiological and the morphological test revealed that the strain MJM 10778 has different characteristics from the strain NBRC 13063. The entire antioxidant assay with the ethyl acetate extract displayed good radical scavenging activity. The IC

Antioxidant capacity and total phenolic contents in hydrophilic extracts of selected Bangladeshi medicinal plants

Objective: To evaluate hydrophilic extracts from edible portions of fifteen plants for total phenolic content (TPC) and anti-oxidant capacity (AC) as an effort to find possible sources for future novel antioxidants. Methods: Folin-Ciocalteau and DPPH radical scavenging assays were employed to determine TPC and AC, respectively. Results: Among the assayed plants, TPC (mean±SD), expressed as gallic acid equivalent, varied from 0.04±0.01 (Amaranthus spinosus) to 6.01±0.04 (Zanthoxylum rhetsa) mg gallic acid equivalent/g fresh weight. AC (mean±SD), expressed as trolox equivalent, ranged from 0.14±0.00 (Alternanthera philoxeroides) to 7.54±0.00 (Zanthoxylum rhetsa) μmol trolox equivalent/g fresh weight. A significant and positive linear relationship (R

In-Vitro Antioxidant potentials in leaves of Coleus aromaticus Benth and rhizomes of Zingiber zerumbet (L.) SM.

Antioxidant activity of aqueous extracts of two common plants, Parnayavani- leaves of Coleus aromaticus Benth and Sthulagranthi- rhizomes of Zingiber zerumbet (L.) Sm. was investigated by adopting various in vitro models such as DPPH assay and Nitric oxide radical scavenging assay. The results of the study show that both plants possesses significant free radical scavenging properties and a clear correlation exists between the antioxidant activity.