RESUMEN
The aim of this paper was to investigate the effect of Huoxiang Zhengqi Oral Liquid on intestinal barrier functions in rats with dampness obstructing spleen-stomach syndrome and primarily explore the mechanism. The rat model of dampness obstructing spleen-stomach syndrome was established, and then the modeled rats were randomly divided into the model control group, Huoxiang Zhengqi Oral Liquid high and low dose groups, and natural recovery group according to gender and body weight, with 10 rats in each group. Another 10 rats were taken as blank control group. After each group received the corresponding treatment for 7 days, rat serum was isolated. D-lactic acid content was detected by the MTT method, and diamine oxidase(DAO) activity was detected by the rate method. Colon tissues of the rats were isolated to detect Na~+-K~+-ATPase activity and Ca~(2+)-Mg~(2+)-ATPase activity by phosphate determination method, glutathione peroxidase(GSH-Px) activity was detected by spectrophotometry, catalase(CAT) activity was detected by ammonium molybdate, superoxide dismutase(SOD) activity was detected by hydroxylamine, the expression of occludin protein and ZO-1 protein was detected by immunofluorescence, and the expression levels of occludin protein and ZO-1 protein were detected by Western blot. RESULTS:: showed that low dose Huoxiang Zhengqi Oral Liquid could improve the body weight, diet, stool and urine state of rats with dampness obstructing spleen-stomach syndrome obviously. The D-lactic acid content and the DAO activity in the serum of rats with dampness obstructing spleen-stomach syndrome were reduced obviously. The activities of Na~+-K~+-ATPase, Ca~(2+)-Mg~(2+)-ATPase, GSH-Px, CAT and SOD in rat colon tissues were increased obviously. The occludin proteins and ZO-1 protein expression levels in rat colon tissues were raised obviously. The differences in the above indexes between Huoxiang Zhengqi Oral Liquid group and the model control group were statistically significant(P<0.05). Huoxiang Zhengqi Oral Liquid could effectively restore the intestinal barrier function in rats with dampness obstructing spleen-stomach syndrome and its mechanism may be related to the repair of intestinal mechanical barrier function.
Asunto(s)
Animales , Ratas , Colon , Mucosa Intestinal , Bazo , EstómagoRESUMEN
Objective To classify the essence of dampness obstructing spleen-stomach syndrome (DOSS) in rats and the effect of Huoxiang Zhengqi Oral Liquid (HZOL) on DOSS. Methods Comprehensive physical methods were applied to establish the rat DOSS model. Body weight, body length, tail length, abdominal girth index, and locomotor activity were observed. Moreover, a proton nuclear magnetic resonance (1H-NMR) based metabonomic approach was developed to profile DOSS-related metabolic perturbations in rat serum and feces and to investigate the intervention effect of HZOL in rats. Results Compared with the healthy control group, rats with DOSS showed weight loss, slow growth of body length, increased abdominal girth index, and decreased locomotor activity along with metabolic perturbations in serum and feces. The disturbed metabolic state associated with DOSS in rat serum and feces could be restored by HZOL. Meanwhile, six and eight potential biomarkers related to DOSS in rat serum and feces, respectively, were reversed by administration of HZOL. Conclusion The current study demonstrated that HZOL exerted protective effect against DOSS in rats, which was probably mediated by amelioration of disturbance in energy and amino acid metabolism and gut microbiota.