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In the current era, medicinal plants are the major resources of indigenous medicines in the traditional medicine system and are playing an important role. Over 80% of the world population mainly depend only on traditional/folk medicinal plants for plant-based medicines to control many of the diseases and their extracts for health care. In the present study, the medicinal plants commonly used by the local people and traditional practitioners of Kappathgudda area, Gadag District, North Karnataka region for treatment of Asthma viz. Acalypha indica L., Datura metel L. and Tylophora indica belongs to Euphorbiaceous, Solanaceae and Asclepiadaceae families were selected for elemental analysis due to its wider application in the traditional medicinal system. Analytical Atomic Absorption Spectrophotometer technique was adopted for the determination of various elements and indicated that Fe, Ca, K, Mg, Ti, Mn, V, Zn, Cu and Cd were present in all samples. The obtained results also revealed that the mineral and trace elemental contents were well within the permissible range and hence the medicinal plants are safe to be utilized in the drug formulations.
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Objective To study the phenylpropanoids from the roots of Datura metel. Methods The separations and purifications were taken by silica gel and ODS chromatogram columns as well as preparative HPLC, and the structural identification based on physicochemical property, 1H-NMR and 13C-NMR as well as HR-MS data. Results Nineteen compounds obtained from the butanol fraction of the 70% ethanol extract of D. metel roots, which were identified as icariside E5 (1), alangisesquin A (2), Glycopentoside F (3), conicaoside (4), (7S,8R) dehydrodiconiferyl alcohol 9’-O-β-glucopyranoside (5), iariciresinol-4’-O-β-D-glucopyranoside (6), 7R,8R-threo-4,7,9-trihy-droxy-3,3’-dimethoxy-8-O-4’-neolignan-9’-O-β-D-glucopyranoside (7), vitrifol A (8), leptolepisol D (9), thero-2,3-bis-(4-hydroxy-3-methoxypheyl)-3-methoxy-propanol (10), hyuganoside IIIb (11), officinalioside (12), iariciresinol-9-O- β-D-glucopyranoside (13), stroside A (14), erythro-buddlenol B (15), sargentodoside D (16), (+)-(7S,8S)-4-hydroxy-3,3’,5’- trimethoxy-8’,9’-dinor-8,4’-oxyneolignan-7,9-diol-7’-oic acid (17), 5’-methoxy lariciresinol (18), and dehydrodiconiferyl alcohol 4-O-β-D-glucopyranoside (19). Conclusion Compounds 2-19 are isolated from Solanaceae family for the first time and compound 1 is firstly isolated from genus Datura L.
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Objective: To study the chemical constituents in the seeds of Datura metel. Methods: The constituents were isolated and purified by silica gel, ODS, and Sephadex LH-20 column chromatographies as well as HPLC. Their chemical structures were elucidated on the basis of spectral data. Results: The compounds were isolated from the EtOAc fraction of D. metel extract and identified as cannabisin D (1), cannabisin E (2), cis-cannabisin E (3), cannabisin F (4), cannabisin L (5), cannabisin G (6), grossamide K (7), hyoscyamilactol (8), daturaolone (9), N-trans-feruloyl tryptamine (10), and fraxetin (11), respectively. Conclusion: Compounds 2 and 4 are firstly isolated from the plants in Solanaceae, compounds 1, 3, and 5-7 are firstly isolated from the plants in genus Datura L. and compounds 8-11 are isolated from this plant for the first time.
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Object To investigate the synthesis of alkaloids by cell suspension cultures of Datura metel L. Methods Cultured cells derived from leaves of D. metel were m aintained in Murashige and Skoog (MS) liquid medium. The effects of different c arbohydrated, different inoculum sizes, and different pH values of medium as aff ecting facters on cell growth and alkaloid formation were studied. Re sults The optimum carbohydrate was sucrose, inoculum size (1.5 - 2. 0 g/L) for fresh weight and initial pH values (5.5-6.0) of the medium were benefit for cell growth and alkaloid synthesis in the suspension cultures. The synthesis of alk aloids was improved when exogenous phenylalanine added to the culture. Conclusion Suspension-cultured cells of D. metel c an be used to synthesize alkaloids.