RESUMEN
Objective To investigate the chemical constituents of Delphinium delavayi. Methods The chemical constituents were separated and purified by column chromatography silica gel, ODS, aluminium oxide, and HPLC. Their structures were elucidated on the basis of extensive spectroscopic analysis, including HR-ESI-MS, NMR experiments and comparison of their spectral data with those described in the literature. Results Thirteen lycoctonine C19-type diterpenoid alkaloids and one isoquinoline alkaloid were isolated from the whole plant of D. delavayi, and the structures were identified as delphatine (1), deltatsine (2), delbonine (3), delbotine (4), lycoctonine (5), delsoline (6), delavaine A (7), delavaine B (8), delsemine A (9), anthranoyllycoctonine (10), gyalanine A and B (11), shawurensine (12), methyllycaconitine (13), and S-glaucine (14) respectively. Conclusion All compounds are isolated from the plants of D. delavayi for the first time. Among them, compound 14 is isolated from the plants of genus Delphinium for the first time.