Relationship between cervical vertebral maturation and dental development in a sample of yemeni children and adolescents / Relación entre la maduración vertebral cervical y el desarrollo dental en una muestra de niños y adolescentes yemeníes

Objective: This study was conducted to investigate the relationship between dental development and cervical vertebral maturation stages in a group of Yemeni children and adolescents. Materials an Methods: The study included digital panoramic radiographs and lateral skull cephalograms obtained from 207 Yemeni subjects­122 females and 85 males aged between 8 to 18 years. Dental maturity was evaluated according to the method of Demirijian et al., calcification stages of the left mandibular canines, first and second premolars and second molars were assessed. Skeletal maturity was assessed by the cervical vertebral maturation (CVM) stages according to the method of Baccetti et al. Correlation between CVM and dental maturation was evaluated by Spearman rank-order correlation coefficient (SROCC). Results: CVM and dental calcification stages were highly correlated (p<0.001) in both genders, ranging from 0.686 to 0.873 for females and 0.787 to 0.871 for males. Calcification stages of the second molars showed the strongest correlation with CVM. Conclusion: Calcification stages of the second molar may be used as a reliable maturation indicator. Dental maturation may be applied to determine the skeletal maturity status of Yemeni children and adolescents.

Objetivo: Este estudio se realizó para investigar la relación entre el desarrollo dental y las etapas de maduración vertebral cervical en un grupo de niños y adolescentes yemeníes. Material y Métodos: El estudio incluyó radiografías panorámicas digitales y cefalogramas laterales del cráneo obtenidos de 207 sujetos yemeníes: 122 mujeres y 85 hombres de entre 8 y 18 años. La madurez dental se evaluó de acuerdo con el método de Demirijian et al. Se evaluaron las etapas de calcificación de los caninos mandibulares izquierdos, primer y segundo premolares y segundos molares. La madurez esquelética se evaluó mediante las etapas de maduración vertebral cervical (CVM) de acuerdo con el método de Baccetti et al. La correlación entre la CVM y la maduración dental se evaluó mediante el coeficiente de correlación de orden de rango de Spearman (SROCC). Resultado: Las etapas de CVM y calcificación dental estuvieron altamente correlacionadas (p<0.001) en ambos sexos, con un rango de 0.686 a 0.873 para las mujeres y 0.787 a 0.871 para los hombres. Las etapas de calcificación de los segundos molares mostraron la correlación más fuerte con CVM. Conclusión: las etapas de calcificación del segundo molar pueden usarse como un indicador de maduración confiable. La maduración dental puede aplicarse para determinar el estado de madurez esquelética de los niños y adolescentes yemeníes.

Comparación de los métodos de Nolla, Demirjian y Moorrees en la estimación de la edad dental con fines forenses / Comparison of Nolla, Demirjian and Moorrees methods for dental age calculation for forensic purposes

RESUMEN: Objetivo: El propósito de este estudio fue comparar tres métodos de estimación de la edad dental (ED) con fines forenses. Material y métodos: Se seleccionaron 512 radiografías panorámicas de sujetos de Maracaibo, estado Zulia, Venezuela, de ambos sexos (272 hembras y 240 varones), con edades cronológicas (EC) entre 6-18 años. Se asignaron los estadios de maduración propuestos por Nolla, Moorrees et al y Demirjian et al a siete dientes mandibulares permanentes del lado izquierdo, la ED fue calculada de acuerdo con la metodología de cada autor. Se obtuvo la EC en la cual se observaron los diferentes estadios de maduración, así como las diferencias de media entre la EC y la ED estimada por cada método mediante un test de Student para muestras relacionadas. Resultados: En general, las hembras alcanzaron los estadios de maduración a edades más tempranas que los varones. Se evidenció en el total de la muestra, una sobreestimación de la edad para el método de Demirjian et al (-0.14 ± 1.45), mientras que para el de Nolla y Moorrees et al se observó una subestimación, esta subestimación fue mayor para el método de Moorrees et al (2.63 ± 2.09) que para el de Nolla (0.42 ± 1.38), siendo que las diferencias encontradas entre la EC y la ED fueron estadísticamente significativas. Conclusión: Se determinó que para el total de la muestra, el método de Demirjian et al fue el más preciso.

ABSTRACT: Objective: The purpose of the present study was to compare three methods for calculation of dental age (DA) to be used for forensic purposes. Material and methods: 512 panoramic X-rays of subjects of both genders living in Maracaibo, State of Zulia, Venezuela were selected (272 females, 240 males). Selected subjects were in the 6-18 years chronological age (CA) range. Maturation stages of Nolla, Moorrees et al and Demirjian et al were assigned to seven permanent teeth of the left side, and DA was calculated according to methodology of each author. CA was obtained where different stages of maturation were observed, as well as mean difference between DA and CA as calculated with each method were obtained with a t student test for related samples. Results: In general, females reached maturation stages at earlier ages than males. The total sample revealed age over-estimation for the Demirjian method (-0.14 ± 1.45), whereas, a sub-calculation was observed for the Nolla and Moorrees et al method. This under-estimation was greater for the Moorrees at al method (2.63 ± 2.09) when compared to Nolla method (0.42 ± 1.38) and differences between DA and CA were found to be statistically significant. Conclusion: In the total studied sample, it was determined that Demirjian et al method was the most accurate.

Morphological characteristics of expressions of DKK1 in different periods of tooth development in postnatal mice and their significances / 吉林大学学报(医学版)

Objective:To study the expression characteristics of Dickkopf1 (DKK1) in different time and space during tooth development of the postnatal mice, and to provide the theoretical basis for clarifying the mechanism of Wnt signaling pathway in regulating the tooth development.Methods:The postnatal Kunming mice at days 0.5, 6.5, 12.5, 18.5, 24.5, and 30.5 respectively after birth were selected and divided into various groups by time,three in each group.The mice in each group were sacrificed and the paraffin sections of mandibular bone including the first molar were prepared at the thickness of 5 μm, followed by HE staining and immunohistochemical staining in order to detect the expressions of DKK1 in tooth tissue and periodontal tissue.Results:At 0.5 d after birth, the mandibular first molar tooth germ was in the bell stage.At 6.5 d the enamel development of mandibular first molar was almost completed, and the epithelium root sheath extended to the root direction.At 12.5 d the dentin development of crown was completed, with the root formatted about 1/3. At 18.5 d the root had formatted about 2/3.At 24.5 d the root had reached the full length.At 30.5 d the apical foramen was narrow, and the root development was basically completed.There was no DKK1 expression at 0.5 d, but it expressed in the odontoblasts and predentin at 6.5 d. From days 12.5 to 30.5,the expressions of DKK1 were positive in periodontal ligament, alveolar bone, and cellular cementum as odontoblasts, which were gradually increased with the prolongation of time.However, no expression of DKK1 was detected in the pulp.Conclusion:DKK1 shows regular expressions at different tooth developmental stages after birth, suggesting its potential role in the growth of dentin and periodontal tissues.

Dentinogenesis Imperfecta: A Case Report of Five Patients in the Same Family Group / Dentinogenesis Imperfecta: Un Reporte de Caso de Cinco Pacientes en el Mismo Grupo Familiar

Dentinogenesis imperfecta (DI) is a type of dentin dysplasia that affects the dentin structure of one or both dentitions, which may be classified in three types. The aim of this report was to show the clinical and radiographic features of the four cases of DI in the same family group. Five brothers were checked clinically and radiographically. Two individuals were diagnosed, by their phenotypic features and medical history, with DI type I; two of them with DI type II and one case without signs of DI. It is important to know the features of dentinogenesis imperfecta to perform a comprehensive dental care, including the right diagnosis and an effective treatment plan.

La dentinogénesis imperfecta (DI) es un tipo de displasia de la dentina que afecta su estructura en una o ambas denticiones. La DI puede clasificarse en tres tipos. El objetivo de este informe fue demostrar las características clínicas y radiológicas de los cuatro casos de DI en un mismo grupo familiar. Cinco hermanos fueron controlados clínica y radiográficamente. Dos individuos fueron diagnosticados, por sus características fenotípicas y antecedentes clínicos, con el tipo de DI I; dos de ellos con DI de tipo II y un caso sin signos de DI. Es importante conocer las características de la dentinogénesis imperfecta para poder realizar una atención odontológica integral, lo que permitirá desarrollar un diagnóstico correcto y un plan de tratamiento efectivo.

Anestesia local odontológica y su influencia en anomalías del desarrollo dental: revisión sistemática de la literatura / Dental local anesthesia and its influence on dental developmental abnormalities: systematic review of the literature

La anestesia local odontológica es un procedimiento de rutina en la práctica odontológica pediátrica que, tal como otros sucesos, podría generar anomalías en el desarrollo dental, considerando la susceptibilidad de los gérmenes dentales en desarrollo; por lo anterior consideramos necesario determinar la existencia de asociación entre anomalías del desarrollo dental y el uso de técnicas anestésicas. Para esto se diseñó un estudio descriptivo basado en búsqueda sistemática de literatura en las bases de datos: Scopus, MEDLINE, Web of Science Core Collection, ProQuest Central, Korean Journal Database y SciELO, utilizando combinación de términos MeSH: ("odontoblasts" OR "ameloblasts" OR "odontogenesis" OR "amelogenesis" OR "dentinogenesis" OR "odontodysplasia" OR "dental enamel hypoplasia" OR "dental sac" OR "dental papilla" OR "enamel organ") AND ("anesthesia" OR "local anesthesia" OR "nerve block" OR "dental anesthesia"). De un total de 96 artículos, se incluyeron para revisión 5 artículos relacionados con los objetivos de esta revisión, excluyéndose las coincidencias. La evidencia presente en la literatura es contradictoria. Existen múltiples diferencias entre los estudios analizados, las que podrían deberse a las técnicas anestésicas involucradas, tipo de anestésico, la no consideración de otros posibles agentes causales (síndromes) o la terapia odontológica. No puede ser determinado una relación entre estos dos aspectos.

The dental local anesthesia is a routine procedure in pediatric dental practice that, as other events could generate abnormalities in dental development, considering the susceptibility of developing tooth germs. From the foregoing it necessary to determine the existence of association between disorders of tooth development and use of anesthetic techniques. A descriptive study based on systematic literature review in databases: Scopus, MEDLINE, Web of Science Core Collection, ProQuest Central, Korean Journal Database and SciELO data was designed, using the following combination of MeSH terms ("odontoblasts" OR "ameloblasts" OR "odontogenesis" OR "amelogenesis" OR "dentinogenesis" OR "odontodysplasia" OR "dental enamel hypoplasia" OR "dental sac" OR "dental papilla" OR "enamel organ") AND ("anesthesia" OR "local anesthesia "OR" nerve block "OR" dental anesthesia"). A total of 96 articles, were included for review a total of 5 articles related to the objectives of this review, excluding the matches. The present evidence in the literature is contradictory. There are many differences between the studies analyzed, which could be due to the anesthetic techniques involved, type of anesthetic, other possible causative agents (syndromes) or dental treatment are not included. It can not be determined a relationship between these two aspects.

Long-term dental follow-up in hematological stem cells transplantation children / Acompanhametnto a longo prazo das alterações dentárias em pacientes pediátricos submetidos ao transplante de celulas tronco hematopoieticas

The aim of this paper is to update the current published content about the craniofacial long-term development disturbances of childhood hematopoietic stem-cell transplantation (HSCT) and the preparative regimens for the onco-hematological malignancies treatments. Methods: Two author sindependently reviewed the published articles about long-term effects of childhood HSCT that fitted into predetermined inclusion/exclusion criteria: clear definition of exposure or intervention, standard outcomes measurement and appropriate statistical analysis. Results: Twelve papers matched all the previous established eligibility criteria and were included in this review. The children’s age at HSCT were related to a higher risk of dental development disturbances, such as agenesis, dental hypoplasia, root stunting, crown-root proportion alterations, and microdontia. Craniofacial vertical growth was impaired in the irradiated patients without antero-posterior or latero-lateral impairment. Temporomandibular joint dysfunction was found to be more prevalent in the patients diagnosed withgraft-versus-host disease. Conclusion: The late effects of craniofacial development disturbances lead to several aesthetic and functional impairment, periodontal bone resorption with consequent impairment of chewing ability, and risk of early tooth loss often associated with life’s quality impairment. Further investigations should be performed to provide accurate information for patients, parent sand health care professionals...

Este artigo visa revisar o conteúdo publicado sobre as alterações tardias em crianças submetidas ao transplante de células-tronco hematopoiéticas(TCTH) e o regime pré-transplante para tratamento das doenças onco-hematológicas. Método: Dois autores independentes conduziram uma revisão simples dos artigos recentemente publicados sobre o tema, utilizando os critérios de exclusão/inclusão pré determinados: definição clara da intervenção clínica realizada, mensuração dos resultados padronizada e análise estatística adequada. Resultados: Doze artigos enquadraram-se em todos os critérios de avaliação pré-determinados para esta revisão. Houve correlação entre a idade da criança no momento do TCTH e maior risco de distúrbios de desenvolvimento dentário, como agenesia, hipoplasia dentária, encurtamento radicular,alterações da proporção coroa-raiz e microdontia.Houve alteração do crescimento craniofacial no sentido vertical sem, no entanto, alterar o crescimento anteroposterior e latero-lateral. Disfunção da articulação temporomandibular foi mais prevalente entre os pacientes com diagnostico de Doença do Enxerto contra o Hospedeiro. Conclusão: Os efeitos tardios do desenvolvimento craniofacial podem levara diversas alterações estéticas e funcionais, reabsorção óssea periodontal, diminuição da capacidade de mastigação e perda dentária precoce com possíveis impactos negativos sobre a qualidade de vida. Mais estudos são necessários para fornecer informações precisas aos pacientes, cuidadores e profissionais de saúde...

Estimación de Edad Dentaria Utilizando el Método de Demirjian en Niños de 5 a 15 Años de Curicó, Chile / Dental Age Estimation Using the Demirjian's Method in Children 5 to 15 Years of Age in Curicó, Chile

El método de Demirjian (MD) es el más ampliamente difundido para estimar la edad dental (ED). Con este método se han realizado numerosos estudios en diferentes grupos étnicos, analizándose niños europeos, asiáticos y norteamericanos entre otros. Estos resultados sugieren posibles diferencias en los patrones de maduración dental entre las diferentes poblaciones. El objetivo del presente estudio fue determinar la edad dentaria aplicando el MD en una muestra de 363 ortopantomografías de pacientes entre 5 y 15 años, obtenidas de un centro radiológico maxilofacial privado en Curicó, VII región, Chile. Se obtuvo un coeficiente de correlación inter-clase (CCI), como medida de correlación entre edad dental y cronológica, de 0,974 (CCI 95% 0,968­0,979). El CCI para niños fue de 0,972 (CCI 95% 0,963- 0,979) y en el caso de las niñas fue de 0,977 (CCI 95% 0,969­0,983). En general, el método de Demirjian tendió a sobreestimar la edad en los niños y niñas. La ED observada fue mayor que la edad cronológica (EC) en 130 niños y en 138 niñas, resultó igual a la EC en 13 niños y 8 niñas, y fue menor que la EC en 38 niños y 33 niñas. En conclusión, nuestros resultados coinciden con hallazgos en numerosos lugares del mundo, es decir, la ED en este grupo se fue levemente mayor que la muestra original franco-canadiense. Sin embargo, el rango obtenido de la ED es similar al de la EC y el grado de correlación entre ambas es casi perfecto al aplicar el CCI. El grado de correlación entre ambas edades para cada sexo también es muy bueno. Por lo tanto, consideramos que el MD es aplicable a esta muestra de niños.

The Demirjian's method (DM) is the most widely used method for estimating dental age. A number of studies with this method have been performed in different ethnic groups, with European, Asian y North American children being analyzed among others. Possible differences in patterns of dental maturation among different population have been suggested. The aim of this study was to determine dental age with the DM in a sample of 363 panoramic radiographs from children 5-15 years old, obtained from a private radiological maxillofacial center in Curicó, Chile. An inter-class correlation coefficient (ICC) was obtained as a measure of correlation between dental and chronological age of 0.974 (ICC 95% 0.968-0.979). The ICC for boys was 0.972 (ICC 95% 0.963- 0.979) and 0.977 for girls (CCI 95% 0.969-0.983). In general, DM overestimated the age in girls and boys. Dental age (DA) was observed to be higher than chronological age (CA) in 130 boys and 138 girls; DA was equal to CA in 13 boys y 8 girls and lower than CE in 38 boys and 33 girls. In conclusion, our study concurs with several other studies in other places of the world, indeed the DA was found to be higher when compared to the original French-Canadian sample. However, the range obtained for DA is similar to CA and the degree of correlation between both is almost perfect when applying ICC. The degree of correlation between both ages for each sex was found as very good. Furthermore, we consider that DM can be applied to this sample of children.

Regional odontodysplasia: a case report / Odontodisplasia regional: relato de caso clínico

PURPOSE: Regional odontodysplasia (RO) is a rare condition characterized by distinctive clinical, radiographic and microscopic findings. It is presumed to be the result of a developmental disturbance that locally affects the odontogenic ectodermal and mesodermal tissues. This report describes the clinical and radiographic findings of RO and its treatment. CASE DESCRIPTION: A 15-year-old boy presented with an uncommon case of RO involving two quadrants of the jaws on the right side of his face. CONCLUSION: A complex multidisciplinary team is required to fully rehabilitate a patient affected by RO. The treatment of RO is controversial and varies according to the individual patient.

OBJETIVO: Odontodisplasia regional (OR) é uma condição rara caracterizada por achados clínicos, radiográficos e microscópicos bem distintos. É o resultado de um distúrbio de desenvolvimento bem localizado afetando tanto o ectoderma quanto o mesoderma odontogênico. Este trabalho descreveu os achados clínicos e radiográficos de um caso de OR e os tratamentos. DESCRIÇÃO DO CASO: Um menino de 15 anos de idade apresentou um caso incomum de OR envolvendo dois quadrantes dos maxilares no lado direito da face. CONCLUSÃO: Há necessidade de uma equipe multidisciplinar complexa para reabilitar inteiramente um paciente com OR. O tratamento de OR é controverso e varia de acordo com as características e resposta do paciente.

Dental development of Didelphis albiventris (Marsupialia): I - incisors and canines

The formation of incisors and canines in marsupials of D. albiventris was studied at various stages of development. Seventy-six specimens, with ages varying from 0 to 100 days, were used in this investigation. Serial sections of the maxilla were obtained in the transverse plane and stained with hematoxylin and eosin. Histological analyses were made to verify the pattern of teeth development, as well as their chronology of eruption. The period of time from birth to 100 days comprised the entire process of teeth development, from epithelial bud formation to early eruption of the teeth. Oral epithelium thickening gave rise to the functional incisors and canines. In addition, a secondary dental lamina emerged in different phases of development in the outer epithelium of incisors and canines, which degenerated when it reached the bud stage. No evidence of deciduous dentition was observed. The results of this investigation suggest that secondary dental lamina represents remnants of a primitive condition in which secondary dentition used to be present.

Estudou-se o desenvolvimento dos dentes incisivos e caninos em 76 amostras de Didelphis albiventris com idade entre 0 e 100 dias. Cortes transversais, seriados de 6 µm de espessura foram obtidos da região da maxila, corados com Hematoxilina e Eosina e analisados ao microscópio de luz. Verificou-se que o período estudado abrange todo o desenvolvimento dental, desde a fase de iniciação da interação epitélio/mesenquima até a completa formação e erupção dos incisivos e caninos. O espessamento do epitélio oral dá origem aos incisivos e caninos funcionais, enquanto o epitélio dental externo do órgão dental origina uma lâmina dental secundária, a qual sofre degeneração, quando o dente alcança o estágio de botão. Não há vestígios de dentição decídua. Sugere-se que a lâmina dental secundária é remanescente de uma condição primitiva na qual ocorria dentição secundária.

The correlation between basic fibroblast growth factor and extracellular matrix in dental pulp during root development of permanent teeth / 实用口腔医学杂志

Objective: To detect the expression of basic fibroblast growth factor(bFGF), type Ⅲ collagen(Ⅲ-col)and fibronectin(FN) in human dental pulp at different root development stages of permanent teeth. Methods: 10 teeth at stage Ri～R 1/4 ,15 at R 1/3 ～R 3/ 4 and 12 at Rc～Ac were sectioned for following examination.bFGF, Ⅲ-col and F N were qualitatively and quantitatively analyzed by the use of immunohistologica l SP method and image analysis technique. Results: The ex pression of bFGF became gradually weaker and weaker with the development of too th root (P