RESUMEN
Background Root conditioning agents reveal the orifices of dentinal tubules and the dentin collagen matrix and provide a substrate which plays a role chemotaxis migration and proliferation of cells involved in periodontal healing and formation of new connective tissue attachment. Materials and methods A total of 15 extracted teeth which were periodontally involved were utilised for the study. All the teeth received 25% citric acid application. The samples were subjected to scanning electron microscope evaluation. The photomicrographs were analysed by image software analyser to assess its surface area. Results Group II showed better results for mean surface area. Conclusion: These results show that citric acid could be a better healing biomaterial in periodontal regeneration by increasing surface area.