Location of DNase Ⅰ hypersensive sites exactly in the promoter region of CD133 in colonel cancer cell line SW480 cells by inverse-PCR / 肿瘤研究与临床

Objective To precious localize DNase Ⅰ hypersensive sites exactly in the promoter region of CD133 of cell line SW480 by inverse-PCR.Methods The colonel cancer cell SW480 nuclei were suspended in digested buffer,treated with DNase Ⅰ at the concentration of 10 U/ml for 10 min.The inversePCR was performed as follows.DNA treated by DNase Ⅰ was purified,fragmented with restricted enzyme EcoRI and Xmal Ⅰ.Then the ends were blunted,ligated by T4 ligase.PCR was performed,and production was sequenced.The restricted enzymes cut sites were near DNase Ⅰ cleavage sites.Results 9 DNase Ⅰ cut sites were identified in CD133 promoter region.The DNaseI hypersensitive sites all distributed in a region -300 bp--700 bp up to transcription start site.Conclusion The DNase Ⅰ cleavage sites could identified preciously by application of inverse-PCR.These sites locate in a region of-300 bp--700 bp up to transcription start site.

Study on the Correlation Between DNaseⅠand G-actin of the Blood and Urine in SLE Patients / 中华皮肤科杂志

0.05);however,the urine G-actin con-centrations were significantly higher in renal involved patients than those of non-renal involved patients(P0.05)in SLE patients.Conclusions The elevated G-actin concentration in the blood of SLE may inhibits the DNaseⅠactivity,which may be one of possible causes to render the decreased DNaseⅠactivity in the blood.

Clinical significance of the determination of deoxyribonuclease Ⅰ and high sensitivity C-reactive protein in CHD patients / 中国实用内科杂志

0.05).However,other groups based on different extent and degree of pathology,clinical risk had significant difference in hs-CRP(P