RESUMEN
Objective To study the effect of 2?-(3-hydroxypropoxy)-Calcitriol (ED-71) on the cell growth and differentiation of human osteoblast-like cell line OS-732. Methods Osteoblast-like cells were cultured with various concentration of ED-71.The effects of ED-71 on proliferation and differentiation were detected by (MTT), alkaline phosphatase activity and level of osteocalcin (bone gla protein,BGP). Results The proliferation rate of osteoblast-like cells cultured for 4 days was significantly inhibited by 10~ -7mol/L concentration of ED-71. ED-71 in 10~ -8, 10~ -9mol/L concentration can significantly increase BGP level, but ED-71 in 10~ -7mol/L concentration may decrease BGP level.Alkaline phosphatase activity compared with control was not changed significantly. Conclusions ED-71 can inhibit cell growth in high concentration and improve cell differentiation in lower concentration.
RESUMEN
Objective: To compare the effects of synthetic 2 ?(3 hydroxypropoxy) calcitriol(ED 71) with 17 ? Estrodiol(E 2) on bone mass, strength and metabolism in ovariectomized(OVX) mice. Methods: 40 female Kunming mice, average 35 g, were randomly divided into 4 groups: OVX group, Sham group, ED 71 group and E 2 group. The latter two groups were also ovariectomized and given ED 71 at 0.4 ?g/(kg?d) and E 2 at 30 ?g/(kg?d) respectively for 6 w. Bone mineral density(BMD), bone mineral content(BMC), bone strength and bone histomorphometric parameters were measured. Results: Compared with OVX mice, in ED 71 and E 2 group, femoral BMD and BMC increased respectively by 3.8%, 5.9% and 3.2%,5.7%; maximum load of femur increased respectively 18.7% and 16%; trabecular bone volume of lumbar vertebra increased respectively 10.6% and 16.1 and serum alkaline phosphatase decreased respectively by 58% and 37%. Conclusion: ED 71 significantly increased BMD, BMC and bone strengh and significantly inhibited bone turnover in OVX mice. Also ED 71 does not induce uterus proliferation.