RESUMEN
Background: The radiation sterilization is one of the best methods for sterilizing vulnerable degradation drugs like cefozopran hydrochloride. Results: Chemical stability of radiosterylized cefozopran hydrochloride, was confirmed by spectrophotometric and chromatographic methods. EPR studies showed that radiation has created some radical defects whose concentration was no more than several dozen ppm. The antibacterial activity of cefozopran hydrochloride irradiated with a dose of 25 kGy was unaltered for Gram-positive bacteria but changed for two Gram-negative strains. The radiation sterilized cefozopran hydrochloride was not in vitro cytotoxic against human CCD39Lu normal lung fibroblast cell line. Conclusions: Cefozopran hydrochloride in solid state is not resistant to radiation sterilization and this method cannot be used for sterilization of this compound.
Asunto(s)
Cefalosporinas/efectos de la radiación , Antibacterianos/efectos de la radiación , Bacterias/efectos de los fármacos , Pruebas de Sensibilidad Microbiana , Supervivencia Celular/efectos de los fármacos , Cefalosporinas/análisis , Cefalosporinas/farmacología , Esterilización , Cromatografía Líquida de Alta Presión/métodos , Espectroscopía de Resonancia por Spin del Electrón , Antibacterianos/análisis , Antibacterianos/farmacologíaRESUMEN
Cu(II) and Ni(II) complexes of 2-butyl-4-chloro-5-formylimidazole thiosemicarbazone (L) are synthesized and characterized by using spectroscopic techniques like elemental analysis, FT-IR, mass spectrometry, electronic and EPR spectra. The complexes are found to have characteristic electronic spectra and the geometry of the complexes are identified as octahedron. Both the complexes are found to exhibit similar anti-microbial activity against the gram –ve and gram +ve bacteria. Anti-cancer activity against the cancer cell lines (MDA-MB 231 cell lines) among the compounds studied for % of viability, the inhibition concentration 50 values were shown by Cu(II)-L complex at 80 mg/ml and by Ni(II)-L complex at 100 mg/ml.
RESUMEN
A novel chelating tridentate organic ligand, 2-Butyl-4-chloro-5-formylimidazole thiosemicarbazone(L) is synthesized and characterized by using spectroscopic techniques like elemental analysis, FT-IR, 1H and 13C NMR, UV-vis and Mass spectrometry. The free ligand is then used for the synthesis of Mn(II) complex thoroughly characterized by FT-IR, EPR and electronic spectral analysis. The complex is found to have characteristic d5 electronic spectrum and the geometry of the complex is identified as octahedron based on the g value obtained from the EPR spectrum. Both the ligand and Mn(II)L2 compounds are found to exhibit similar antimicrobial activity against the gram –ve and gram +ve bacteria.