RESUMEN
Objective To investigate the effects of ER-α36 on invasion of human gastric cancer cell lines SGC7901 by miR-143. Methods Lentiviral vectors were constructed to generate up-and down-regulations of miR-143 lentiviruses (LV-miR-143 and LV-anti-miR-143,respectively).The viruses were used to infect human gastric cancer cell lines SGC7901.The ER-α36 protein expression level and the invasion of constructed cells were detected by Western blot and transwell. The target gene of miR-143 was predicted by bioinformatics tools.Luciferase reporter assay was carried out to confirm the predicted target gene. Results The infection efficiency of the lentivirus titers of LV-miR-143 and LV-anti-miR-143 were over 80% shown by the green fluorescence. The ER-α36 expression level,the cell invasion in LV-miR-21 group were significantly lower than those in LV-anti-miR-21 group(P<0.05). Conclusions miR-143 plays an important role in the negative control of gastric cancer invasion by the regulation of ER-α36.
RESUMEN
Estrogen receptor-alpha 36 (ER-α36), which is a new type of ER with a relative molecular weight of 3.57×104, has been demonstrated to have many important physiological functions in different tissues and organs in recent years. Many studies have shown that the occurrence and development of cancer are closely related to the expression level of ER-α36, which suggests that ER-α36 may serve as a potential therapeutic target in the treatment of some cancers. This paper reviews the advances in research on the correlation between ER-α36 and some cancers such as breast cancer, gastric cancer, liver cancer, and so on.