Analysis of the Electrophoretic Profiles of Prion Protein in Carcinous and Pericarcinous Lysates of Six Different Types of Cancers / 生物医学与环境科学（英文）

Objective@#To find the different electrophoretic profiles of prion protein in carcinous and individual pericarcinous tissues in lysates of gastric, colon, liver, lung, thyroid, and laryngeal cancers.@*Methods@#Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot were used to test the amounts and electrophoretic patterns of total PrP and the tolerance of PK (protease K) digestion among six various cancer tissue types.@*Results@#A mass of PrP signals with a large molecular weight were identified in the homogenates of peripheral tissues. The amounts and electrophoretic patterns of total PrP did not differ significantly between carcinous and pericarcinous tissues. PrPs in all types of the tested cancer samples were PK sensitive but showed diversity in the tolerance of PK digestion among various tissue types.@*Conclusions@#The study revealed that the included electrophoretic patterns of carcinous and pericarcinous tissues were almost similar. Unlike PrP-specific immunohistochemical assay, evaluation of PrP electrophoretic patterns in the peripheral organs and tissues by Western blot does not reflect tumor malignancy.

Detecção de rotavírus em um cão doméstico na cidade de Manaus-AM / Rotavirus detection in a domestic dog in the City of Manaus-AM

Os rotavírus do grupo A, são frequentemente associados com gastroenterites em mamíferos e aves. O objetivo deste trabalho foi detectar a presença de rotavírus em fezes de cães sintomáticos e assintomáticos para diarréia aguda. Foram coletadas 32 amostras de fezes de cães. Todas as amostras foram submetidas à extração do RNA viral seguida da Eletroforese em Gel de Poliacrilamida (EGPA), onde se identificou apenas 1 (um) caso de infecção por rotavírus, em amostra assintomática. A análise do eletroferótipo mostrou um perfil 4:2:3:2 longo, e a homologia dos eletroferótipos de rotavírus humano e canino foi muito alta, sugerindo uma possível infecção interespécie.

The group A rotaviruses are frequently associated with gastroenteritis in mammals and birds. The objective of this study was to detect the presence of rotavirus in feces of symptomatic and asymptomatic dogs for acute diarrhea. 32 samples of dog feces were collected. All the samples were submitted to viral RNA extraction followed by electrophoresis in poliacrylamide gels (PAGE), where only one case of rotavirus infection in one asymptomatic sample was found. The electrophoretic analysis showed 4:2:3:2 long profile, and the homology between human and dog rotaviruses was very high. This suggests a possible interspecies infection.