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Objective: To investigate the relationships of RWD-containing sumoylation enhancer (RSUME) and hypoxia-inducible factor-1α (HIF-1α)/vascular endothelial growth factor (VEGF) pathway with invasion of pituitary adenoma. Methods: The expression levels of RSUME, HIF-1α and VEGF-A mRNAs in human pituitary adenoma tissues were detected by real-time fluorescence quantitative-PCR. The expression levels of small ubiquitin-related modifier-1 (SUMO-1), HIF-1α and VEGF-A proteins in pituitary adenoma tissues were detected by Western blotting. After transfecting with small interfering RNA (siRNA)-targeting RSUME into murine pituitary adenoma AtT-20 cells, the expression levels of RSUME, HIF-1α and VEGF-A mRNAs as well as SUMO-1, HIF-1α and VEGF-A proteins were detected by real-time fluorescence quantitative-PCR and Western blotting, respectively. The invasion ability was measured by Transwell assay. Results: The expression levels of RSUME, HIF-1α and VEGF-A mRNAs as well as SUMO-1, HIF- 1α and VEGF-A proteins in invasive pituitary adenoma tissues were significantly higher than those in non-invasive pituitary adenoma tissues and normal pituitary tissues (all P < 0.05). The SUMO-1 expression was positively correlated with HIF-1α expression in invasive pituitary adenoma tissues (r = 0.687, P < 0.05), and HIF-1α expression was positively correlated with VEGF-A expression (r = 0.773, P < 0.05). The expression levels of RSUME and VEGF-A mRNAs as well as SUMO-1, HIF-1α and VEGF-A proteins in AtT-20 cells after transfection with RSUME siRNA were significantly lower than those in the negative control group (AtT-20 cells transfected with negative control siRNA) and the blank control group (AtT-20 cells without any transfection) (all P < 0.05). The invasion ability of AtT-20 cells after transfection with RSUME siRNA was significantly inhibited (P < 0.05). Conclusion: RSUME can promote the invasion of pituitary adenoma by regulating HIF-1α/VEGF pathway. Silencing of RSUME gene may inhibit the invasion of pituitary adenoma cells.
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Objective To observe endothelial and fibfinolytic functions of clinical AF patients and effects of cilazapril on it and to investigate the mechanism and treatments of thrombogenesis in AF.Methods 63 AF patients were randomly di- vided into control group (n=30) and cilazapril group (n=33).All patients received general treatment according symptoms.In addition,the patients in cilazapril group received cilazapril (2.5mg/d,2 weeks).Plasma Ang Ⅱ, vWF,t-PA and PAI-1 level were measured before and after 2 weeks.There were 36 patients with sinus rhythm (SH) in non-AF control group.Results AF patients had higher plasma AnglI level than SH patients that indicated RAS acti- vated[(107.7?22.0) ng/L vs (70.2?15.8) ng/L,P
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Objective To observe endothelial and fibrinolytic functions of clinical AF patients and effects of cilazapril on it and to investigate the mechanism and treatments of thrombogenesis in AF.Methods 63 AF patients were randomly divided into control group(n=30)and cilazapril group(n=33).All patients received general treatment according symptoms.In addition,the patients in cilazapril group received cilazapril(2.5mg/d,2 weeks).Plasma Ang Ⅱ, vWF,t-PA and PAI-1 level were measured before and after 2 weeks.There were 36 patients with sinus rhythm (SH)in non-AF control group.Results AF patients had higher plasma Angll level than SH patients that indicated RAS activated[(107.7?22.0)pg/mL vs(70.2?15.8)pg/mL,P
RESUMEN
It is recommened to remove the arterial adventitia from the vessel ends to prevent adventitial interpose between the edges of anastomoses vessels. We have analysed the effect of adventitial removal on the patency in microvascular anastomosis. using rat femoral artery Three groups of adventitial removal methods were studied: non-removal group, sharp dissection group and blunt dissecction group. Patency was evaluated one hour, 24hours and 7days after anastomosis. The patency rate of non-dissection group(n=40) was 90% one hour after anastomosis but it reduced to 62.5% both at 24 hours and at 7days because interpositioning of adventitia induced thrombosis. The blunt skipping group showed 62.5% of patency rate at one hour most likely due to vascular spasm, but after 24hours it was recovered to 80% and 78.5% at 7th day. The sharp dissection group showed persistently high patency rate of 97.5% after one hour,95% both after 24 hours and after 7 days. Scanning electron microscopy showed endothelial loss and medial edema in bluntly stripped vessels but it showed well preserved endothelial cells and medial wall in sharp dissection group. The reason of relatively low patency rates of blunt dissection group compared with sharp dissection group 24 hours and 7 days after anastomosis(p=0.043 and p=0.023, respectively) was endothelial damage induced by stretching force made during blunt stripping.