RESUMEN
Objective To explore the cell viability in the ablation area of thyroid nodules at 6 months after microwave ablation by enzyme histochemical staining. Methods Twenty-four ablation areas of thyroid nodules were selected from 20 patients who underwent histopathological assessment of the ablation area by core needle biopsy at 6 months after microwave ablation between Dec. 2017 and Sep. 2018. Core needle biopsy was performed at the central and marginal regions of the ablation area with a cutting biopsy needle. The specimens were obtained and placed in liquid nitrogen to make frozen sections. Enzyme histochemical staining was used to detect the activities of succinate dehydrogenase (SDH) and nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d), and the difference of cell morphology and histological structure was compared with H-E staining results. Results The specimens of the central and marginal regions of 24 ablation areas were successfully obtained. The histochemical staining of SDH and NADPH-d in the central region of ablation area had good consistency, and the negative rates were both 95.83% (23/24). The histochemical staining of SDH and NADPH-d in the marginal region of ablation area also had good consistency, and the negative rates were both 91.67% (22/24). H-E staining of 23 central regions and 22 marginal regions showed pink amorphous mass of necrosis. H-E staining of 1 central region and 2 marginal regions showed partly necrotic and fibrous tissue hyperplasia. The location of fibrous tissue hyperplasia was consistent with the location of the positive region of enzyme histochemical staining. Conclusion At 6 months after microwave ablation, the tissue in the ablation area of thyroid nodules is consistent with coagulative necrosis, and is still inactivated. SDH or NADPH-d enzyme histochemical staining combined with H-E staining can objectively evaluate the old ablation area.
RESUMEN
Objective;To investigate whether the traditional Chinese medicine Yisheng Injection can reduce the injury on isolated rat heart induced by cold ischemia. Methods ;18 isolated female closed colony SD rats were divided into two groups at random. In the control group, the isolated rat hearts were preserved in 0.9% sodium chlorine solution at 4℃ , while in the experimental group ,the hearts were preserved in 0.9% sodium chlorine solution containing Yisheng injection at 4℃. The specimens were harvested 2,6,12, 24,48 and 72 hours after preservation. Histochemical changes were observed in two groups. Results ;In the control group, the activities of alkaline phosphatase ( ALP) and NADPH diaphorase (NADPHD) decreased after cold ischemia for 24 hours. Lactic dehydro-genase (LDH) decreased slightly, while ALP and NADPHD decreased evidently, after cold ischemia for 48 hours. Succinic dehydro-genase ( SDH) and cytochrome oxidase ( CCO) decreased slightly, and NADPHD disappeared after cold ischemia for 72 hours respectively. In the experimental group, NADPHD, LDH and CCO decreased slightly after cold ischemia for 24, 48 and 72 hours. ALP decreased slightly after cold ischemia for 48 hours, however, SDH showed no changes, after cold ischemia for 72 hours. Conclusion : Cold ischemia can induce cold ischemia injury in isolated rat hearts, especially the vascular endothelial cells being more sensitive. Yisheng injection can reduce this kind of injury to some degree.
RESUMEN
Objective:To investigate the injury on isolated cadaver testes induced by ischemia-reperfusion (I/R).Methods:13 isolated cadaver testes contributed by 13 persons were preserved under 0℃～4℃ hypothermia and then reperfused under 37℃.Histology and histochemical changes were observed.Results:4℃ cold ischemia in 18 hours only could induce trivial swelling and vascular degeneration of endothelial cells(ECs).Obvious pathologic changes occurred after 24 hours,including detachment of ECs,separation between basement membrane and seminiferous epitelium,malalignment of spermatogenous cell and edema of mesenchyme.Injury was worse along with the prolongation of cold preservation time.Changes of LDH and SDH activities were found by histochemical staining.Reperfusion following 6 hours ischemia induced tissue injury and unusual enzyme activity.All changes were more obvious after reperfusion following 12,18,24 or 36 hours of cold ischemia.Conclusion:HCA can preserve testes for 18 hours.Testes can't keep normal histological and histochemical structure beyond 24 hours of preservation under 4℃ hypothermia,and 37℃ reperfusion can make injury worse.
RESUMEN
Objective Peritoneal lymphatic features are studied to provide necessary data for understanding transport capabilities of the endothelial and mesothelial cells. Methods Enzyme\|histochemical staining methods were employed to investigate organization of the lymphatic networks and their endothelial ultrastructures in the monkey peritoneum using light,scanning and transmission electron microscopy. Results 5' Nase positive initial lymphatics showed extensive network,obvious valve like structures and numerous blind ends.The calibre of lymphatics with extremely irregular lumen varied greatly from 40 to 120?m.Lymphatic endothelium was usually separated from mesothelium by a small quantity of loose connective tissue,or they directly contacted each other in some areas.No basal lamina occurred between the peritoneum and the lymphatics originating from milky spots in the omentum and mesovarium.Milky spots are oval or round visible bodies aggregated by macrophages and lymphocytes.Both the endothelium of lymphatic lacunae and the mesothelium that forms the peritoneal stomata,represent a strong 5' Nase activity.Abundant microfibrils attached the stomatal edge. Conclusion The distribution and structure of peritoneal initial lymphatics reveal significant regional variations,and lymphatic endothelium has a close morphological and functional relationship with the mesothelium. [