RESUMEN
Objective: To study the chemical constituents in green walnut husks of Juglans regia. Methods: The chemical constituents were separated and purified by silica gel column chromatography and HPLC. Their structures were determined on the basis of spectroscopic analyses. Results: Fifteen compounds were isolated and the structures were identified as epi-dihydrophaseic acid (1), 4-butoxy-5, 8-dihydroxy-3, 4-dihydronaphthalen-1-one (2), 4-ethoxy-5, 8-dihydroxy-3, 4-dihydronaphthalen-1-one (3), myricatomento-genin (4), nodulisporone (5), 5, 8-dihydroxy-4S-methoxy-β-tethalone (6), 5-hydroxy-4-methoxy-α-naphthalen-1-one (7), isosclerone (8), 4, 5, 8-trihydroxy-1, 2, 3, 4-tetrahydronaphthalene-1-one (9), 1-ethyl malate (10), 1-buthyl malate (11), succinic acid (12), ethyl-O-β-D-glucopyranoside (13), 1α, 2α, 4β-trihydroxy-1, 2, 3, 4-tetrahydronaphthalene (14), and L-2-O-methyl-chiroinosicol (15). Conclusion: Compounds 5 and 10-14 are isolated from the green walnut husks of J. regia for the first time, and compounds 1, 4, and 15 are isolated from this plant for the first time.
RESUMEN
Objective: To investigate the chemical constituents in the shells of Juglans sigillata. Methods: The chemical constituents were isolated by silica gel, RP18, Sephadex LH-20, and MCI column chromatography and semi-preparative HPLC and so on. The structures were identified on the basis of spectroscopic analysis and chemical evidence. Results: Fifteen compounds were isolated and identified in the 70% ethanol extract from the shells of J. sigillata including seven phenolic glycosides: tachioside (1), mudanoside A (2), 4-O-β-D-glucopyranosylvanillc acid (3), breynioside A (4), 1-O-vanilloyl-β-D-glucose (5), 6'-O-vanilloyltachioside (6), and 6'-O-vanilloylisotachioside (7); three phenylpropanoide acid glycosides: 6-O-feruloyl-D-glucopyranose (8), methyl-4-O-coumaroylquinate (9), and 5-p-cis-coumaroylquinic acid (10); two tetralone glycosides: juglanin A (11) and juglanin E (12); one norsesquiterpenes glycoside: roseoside (13); one flavone: toxifolin (14); and one glucosylated abscisic acid derivate: (1'R, 3'R, 5'R, 8'S)-epi-dihydrophaseic acid β-D-glucoside (15). Conclusion: Except compound 14, the other compounds are isolated from the shells of J. sigillata for the first time. And compounds 1-4, 13, and 15 are reported for the first time from the plants in genus of Juglans L.