RESUMEN
Objective To investigate the expression of Epstein-Barr virus(EBV)-latent membrane protein 1 (LMP1) in chronic atrophic gastritis (CAG) with intestinal metaplasia and discuss its effect on gastric cancer.Methods Immunohistochemistry was used to examine the expression of EBV-LMP1 in 45 cases of chronic superficial gastritis(CSG),63 cases of CAG with intestinal metaplasia and 36 cases of gastric cancer.Results There was no expression of EBV-LMP1 in CSG and gastric cancer,while the positive rate of EBV-LMP1 in CAG with intestinal metaplasia was 36.5％ (23/63) and EBV-LMP1 was mainly stained in the cell nucleus.The expression of EBV-LMP1 in CAG with intestinal metaplasia was significantly higher than that in CSG and gastric cancer,and there was significant difference (P =0.000).Conclusions EBV-LMP1 is expressed in CAG with intestinal metaplasia.The expression of EBV-LMP1 is significantly higher than that in CSG and gastric cancer indicating that EBV infection in gastric carcinogenesis may play an important role in the early stages.
RESUMEN
Objective: To investigate the effects of Epstein-Barr virus-latent membrane protein 1 (LMP1) and p38 mediating epithelial-mesenchymal transition of nasopharyngeal carcinoma (NPC), and to elucidate its relationship with tumor metastasis. Methods: The expression levels of E-cadherin, vimentin, LMP1 and p38 in NPC tissues and cell lines were detected by immunohistochemistry, immunocytochemistry, RT-PCR and Western blotting. The invasion and migration abilities of NPC cell lines were examined by Transwell assay. Results: The positive expression rates of E-cadherin in different NPC tissues and lymph node metastases were decreased (P<0.01), whereas the positive expression rates of vimentin and LMP1 were increased (P<0.001). The difference in the positive expression rate of p38 was not statistically significant (P<0.05).The expression of LMP1 was negatively associated with the expression of E-cadherin in NPC tissues (P<0.05). In cervical lymph node metastasis, the expression of LMP1, which had no association with the expression of E-cadherin (P<0.05), was associated with the expression of vimentin (P<0.05). The expressions of E-cadherin mRNA and protein were suppressed in CNE1-GL and CNE2Z cells, whereas the expressions of vimentin mRNA and protein were increased. The expression level of LMP1 protein was high in CNE1-GL and CNE2Z cells, and the expression level of p38 protein was high in CNE1-GL cells. Transwell assay showed that LMP1 could promote the migration and invasion abilities of NPC cells (P<0.001). Conclusion: LMP1 can induce EMT and enhance the metastatic potential of nasopharyngeal carcinoma. Copyright© 2011 by Tumor.