RESUMEN
OBJECTIVE@#To investigate whether Ershen Pill (ESP, ) could alleviate the symptom of Pi (Spleen)-Shen (Kidney) yang deficiency (PSYD)-induced diarrhea in rat model and explore its anti-diarrhea mechanism.@*METHODS@#Seventy-five Sprague-Dawley rats were divided into 5 groups by a random number table, including control, positive, model, low-dose (LD) and high-dose (HD) ESP groups, 15 rats in each group. All the rats, except those in the control group, were developed PSYD induced-diarrhea based on its pathology and etiology. The rats in positive, LD and HD ESP groups were treated with Shenling Baizhu Pill (), LD (1.05 g/kg) or HD (3.50 g/kg) ESP petroleum ether extract once a day for 2 weeks, respectively. Body weight change and diarrhea index were measured. The histology scores of the kidney were evaluated via hematoxylin and eosin (HE) staining. Aquaporin-3 (AQP3) expression in the colon was analyzed by immunofluorescence, quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and Western blot, respectively.@*RESULTS@#Compared with the model group, oral administration of LD and HD ESP prevented body weight loss and inhibited diarrhea after 2-week treatment (P<0.05). Kidney deterioration was impeded, and the histology score in LD and HD ESP groups were 8.2 and 10.5, respectively, which were both higher than those in the model group (P<0.05). In addition, ESP treatment alleviated rat colitis, and HD ESP significantly improved the AQP3 positive staining intensity in the colon tissue compared with the model group. The result from Western blot revealed that AQP3 protein synthesis in colon tissue of LD and HD ESP groups increased by 2.1- and 5.9-fold compared with the model group (P<0.05). qRT-PCR result showed that AQP3 gene expression in the HD ESP group was also up-regulated by 2.5-fold normalized to the model group (P<0.05).@*CONCLUSION@#ESP extract effectively alleviates the symptoms of PSYD and relieves PSYD-induced diarrhea by improving AQP3 synthesis in the colon.
RESUMEN
Objective: To clarify the contribution of the fingerprint peaks from different parts of prepared Ershen Pill to the effect of warming spleen to relieve diarrhea, and to reflect the material basis. Methods: The HPLC fingerprints of different parts of Ershen Pill (composed of Psoraleae Fructus stir-baked with saltwater and Myristica Fragrans simmered with bran) were established. The diarrhea index of acute diarrhea in mice caused by senna leaf was researched to compare the effect of warming spleen to relieve diarrhea. Results: The fingerprints of different parts of Ershen Pill were established, and all of the similarity degrees were over 0.90. The effect of warming spleen to relieve diarrhea of Ershen Pill was obtained from many constituents collaboratively. The contribution of different peaks to relieve diarrhea with astringents was ranked as 6 > 18 > 7 > 20 > 14 > 21 > 1 > 25 > 24 > 23 > 4 > 3 > 8 > 11. Conclusion: The method for establishing HPLC fingerprints of different parts of Ershen Pill is simple and repeatable. The relationship between the fingerprints and the effect of warming spleen to relieve diarrhea is paralleled to some extent. And this will lay a foundation for the research of processing mechanism of Ershen Pill.