RESUMEN
Objective: To observe the effects of ethyl acetate extract from Huanglian Jiedu Decoction (EAHJD) on the virulence factors of Candida albicans. Methods: Egg-yolk medium, milk-plate medium, and olive oil emulsification were used respectively to test the activities of phospholipase (PL), aspartic protease (Sap), and lipase (Lip) of C. albicans. The water-hydrocarbon two-phase assay was applied to measure the cell surface hydrophobicity (CSH) of C. albicans. qRT-PCR was adopted to observe the expression of virulence factors related genes. Results: EAHJD had no effect on the activity of PL. EAHJD (1 250 μg/mL) could significantly inhibit the activity of PL and Lip better than that by 312 μg/mL EAHJD. EAHJD could reduce CSH of C. albicans in a dose-independent manner and CSH1 was down-regulated by 7.69, 3.57, and 2.95 folds by 1 250, 312, and 78 μg/mL EAHJD, respectively. The expression of secretory enzyme related genes displayed different changing folds: PLC1, Sap2, Sap3, Sap9, Lip3, Lip4, and Lip6 were down-regulated; PLB1, PLC2, Sap1, Sap10, and Lip5 had no distinct change treated by EAHJD. Conclusion: EAHJD could inhibit the activities of virulence factors of C. albicans.